Publications de Lina Carlini | EPFL Graph Search

Mitochondrial membrane tension governs fission

Suliana Manley, Lina Carlini, Dora Mahecic, Tatjana Isabelle Kleele

During mitochondrial fission, key molecular and cellular factors assemble on the outer mitochondrial membrane, where they coordinate to generate constriction. Constriction sites can eventually divide or reverse upon disassembly of the machinery. However, a ...

CELL PRESS2021

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size

Suliana Manley, Thaïs Reichler, Lina Carlini, Anna Archetti, Charlotte Sophia Floderer

Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms s ...

2019

Exploring dynamic organellar shape using live-cell fluorescence super-resolution microscopy

Lina Carlini

Eukaryotic cells contain membrane-bound organelles, that perform specialized tasks, which control cellular fate. Organelles are dynamic and contain nanometre-scale, ultrastructural features; in fact, their shape and function are interconnected. Thus, there ...

EPFL2016

Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Suliana Manley, Seamus John Holden, Lina Carlini, Kyle Michael Douglass

Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, whi ...

Public Library of Science2015

Fast live cell imaging at nanometer scale using annihilating filter based low rank Hankel matrix approach

Michaël Unser, Suliana Manley, Lina Carlini

Localization microscopy such as STORM/PALM can achieve a nanometer scale spatial resolution by iteratively localizing fluorescence molecules. It was shown that imaging of densely activated molecules can accelerate temporal resolution which was considered a ...

Spie-Int Soc Optical Engineering2015

3D high-density localization microscopy using hybrid astigmatic/biplane imaging and sparse image reconstruction

Michaël Unser, Suliana Manley, Seamus John Holden, Lina Carlini

Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs ...

Optical Soc Amer2014

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Luc Reymond, Grazvydas Lukinavicius, Suliana Manley, Aleksandr Benke, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

FALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data

Michaël Unser, Cédric René Jean Vonesch, Suliana Manley, Hagay Kirshner, Seamus John Holden, Lina Carlini

Super resolution microscopy such as STORM and (F) PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow te ...

Nature Publishing Group2014

Live Intracellular Super-Resolution Imaging Using Site-Specific Stains

Suliana Manley, Lina Carlini

Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological feature ...

American Chemical Society2013