Publications de Lorenz Jan Steinbock

Manufacturing of orifices in glass like materials, e.g. Nanocapillaries, and objects obtained according to this process

Aleksandra Radenovic, Lorenz Jan Steinbock

The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique14,17. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules12,31. However ...

2016

The emergence of nanopores in next-generation sequencing

Aleksandra Radenovic, Lorenz Jan Steinbock

Next-generation sequencing methods based on nanopore technology have recently gained considerable attention, mainly because they promise affordable and fast genome sequencing by providing long read lengths (5 kbp) and do not require additional DNA amplific ...

2015

Probing the size of proteins with glass nanopores

Aleksandra Radenovic, Lely Feletti, Melanie Blokesch, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

Single molecule studies using nanopores have gained attention due to the ability to sense single molecules in aqueous solution without the need to label them. In this study, short DNA molecules and proteins were detected with glass nanopores, whose sensiti ...

Royal Soc Chemistry2014

ComEA Is Essential for the Transfer of External DNA into the Periplasm in Naturally Transformable Vibrio cholerae Cells

Aleksandra Radenovic, Matteo Dal Peraro, Melanie Blokesch, Patrick Martin Seitz, Hasan Pezeshgi Modarres, Lorenz Jan Steinbock, Roman Bulushev

The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculativ ...

Public Library Science2014

Measurement of the Position-Dependent Electrophoretic Force on DNA in a Glass Nanocapillary

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev, Sergey Khlybov

The electrophoretic force on a single DNA molecule inside a glass nanocapillary depends on the opening size and varies with the distance along the symmetrical axis of the nanocapillary. Using optical tweezers and DNA-coated beads, we measured the stalling ...

American Chemical Society2014

Combination of Optical Tweezers with Nanocapillaries as System for Estimation of DNA/Ligand Interactions

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev

Cell Press2014

Shrinking Nanocapillaries to Low Noise Nanopores for Single Molecule Detection

Aleksandra Radenovic, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

Cell Press2014

DNA Trans location through Low-Noise Glass Nanopores

Aleksandra Radenovic, Camille Alice Raillon, Lorenz Jan Steinbock, Roman Bulushev, Swati Krishnan

The effect of electron irradiation-induced shrinking on glass nanocapillaries with diameters ranging from 75 to 14 nm was analyzed by measuring the conductance characteristics with and without DNA translocation. We have investigated nanocapillary shrinking ...

American Chemical Society2013

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Aleksandra Radenovic, Lorenz Jan Steinbock

The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules. However, this has ...

American Chemical Society2013

Lipid-coated nanocapillaries for DNA sensing

Lorenz Jan Steinbock, Vivek Vijay Thacker

We report a simple and efficient way to accomplish the chemical modification of glass nanopores by means of lipid self-assembly. Lipid coating improves the success rate of these glass nanopores as biosensors to detect lambda-DNA. ...

Royal Soc Chemistry2013

Fast and automatic processing of multi-level events in nanopore translocation experiments

Aleksandra Radenovic, Michael Graf, Camille Alice Raillon, Lorenz Jan Steinbock

We have developed a method to analyze in detail, translocation events providing a novel and flexible tool for data analysis of nanopore experiments. Our program, called OpenNanopore, is based on the cumulative sums algorithm (CUSUM algorithm). This algorit ...

Royal Society of Chemistry2012