Publications de Riccardo Sinisi

A bioluminescent probe for longitudinal monitoring of mitochondrial membrane potential

Elena Goun, Arkadiy Bazhin, Riccardo Sinisi, Ghyslain Martial Alain Budin, Tamara Maric

Mitochondrial membrane potential (Delta Psi(m)) is a universal selective indicator of mitochondrial function and is known to play a central role in many human pathologies, such as diabetes mellitus, cancer and Alzheimer's and Parkinson's diseases. Here, we ...

NATURE RESEARCH2020

Azacyanine dyes and use thereof

Elena Goun, Riccardo Sinisi, Alma Rosa Morales Morales

The application provides fluorescent dyes, which are cyanine dyes that incorporate additional aza moieties in the indolenium heterocycles and/or in the methine chains connecting them. Symmetrical and unsymmetrical chemically reactive azacyanine dyes are de ...

2019

Azacyanine dyes and use thereof

Elena Goun, Riccardo Sinisi, Alma Rosa Morales Morales

The application provides fluorescent dyes, which are cyanine dyes that incorporate additional aza moieties in the indolenium heterocycles and/or in the methine chains connecting them. Symmetrical and unsymmetrical chemically reactive azacyanine dyes are de ...

2019

Bioluminescent-based imaging and quantification of glucose uptake in vivo

Elena Goun, Arkadiy Bazhin, Riccardo Sinisi, Pavlo Khodakivskyi, Georgy Mikhaylov, Aleksey Yevtodiyenko, Tamara Maric

Glucose is a major source of energy for most living organisms, and its aberrant uptake is linked to many pathological conditions. However, our understanding of disease-associated glucose flux is limited owing to the lack of robust tools. To date, positron- ...

NATURE PUBLISHING GROUP2019

Azacyanine dyes and use thereof

Riccardo Sinisi, Alma Rosa Morales Morales

The application provides fluorescent dyes, which are cyanine dyes that incorporate additional aza moieties in the indolenium heterocycles and/or in the methine chains connecting them. Symmetrical and unsymmetrical chemically reactive azacyanine dyes are de ...

2018

Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection

Riccardo Sinisi, Ghyslain Martial Alain Budin, Pavlo Khodakivskyi

Introduction: Diagnosing-prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for ...

Lippincott Williams & Wilkins2017

Compact solid-state CMOS single-photon detector array for in vivo NIR fluorescence lifetime oncology measurements

Claudio Bruschini, Edoardo Charbon, Krisztian Homicsko, François Eric Powolny, Riccardo Sinisi, Harald Arjan Robert Homulle, David Li

In near infrared fluorescence-guided surgical oncology, it is challenging to distinguish healthy from cancerous tissue. One promising research avenue consists in the analysis of the exogenous fluorophores' lifetime, which are however in the (sub-)nanosecon ...

2016

Pre-clinical Evaluation of a Cyanine-Based SPECT Probe for Multimodal Tumor Necrosis Imaging

Riccardo Sinisi

Recently we showed that a number of carboxylated near-infrared fluorescent (NIRF) cyanine dyes possess strong necrosis avid properties in vitro as well as in different mouse models of spontaneous and therapy-induced tumor necrosis, indicating their potenti ...

Springer2016

The Necrosis-Avid Small Molecule HQ4-DTPA as a Multimodal Imaging Agent for Monitoring Radiation Therapy-Induced Tumor Cell Death

Riccardo Sinisi

Purpose: Most effective antitumor therapies induce tumor cell death. Non-invasive, rapid and accurate quantitative imaging of cell death is essential for monitoring early response to antitumor therapies. To facilitate this, we previously developed a biocom ...

Frontiers Media Sa2016

Fluorescence lifetime imaging to differentiate bound from unbound ICG-cRGD both in vitro and in vivo

Claudio Bruschini, Edoardo Charbon, François Eric Powolny, Riccardo Sinisi, Harald Arjan Robert Homulle

Excision of the whole tumor is crucial, but remains difficult for many tumor types. Fluorescence lifetime imaging could be helpful intraoperative to differentiate normal from tumor tissue. In this study we investigated the difference in fluorescence lifeti ...

Spie-Int Soc Optical Engineering2015

Matrix-Assisted Transplantation of Functional Beige Adipose Tissue

Riccardo Sinisi, Grigory Karateev

Novel, clinically relevant, approaches to shift energy balance are urgently needed to combat metabolic disorders such as obesity and diabetes. One promising approach has been the expansion of brown adipose tissues that express uncoupling protein (UCP) 1 an ...

American Diabetes Association2015

Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

Claudio Bruschini, Edoardo Charbon, Douglas Hanahan, Krisztian Homicsko, François Eric Powolny, Riccardo Sinisi, Harald Arjan Robert Homulle

We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility ...

Spie-Int Soc Optical Engineering2014

Bioluminescence imaging of small biomolecules

Aurélien Godinat, Elena Goun, Riccardo Sinisi, Grigory Karateev, Aleksandra Konovalova, Jens Tomas Frigell, Ghyslain Martial Alain Budin

The invention relates to a technique to detect small molecules using Bioluminescence imaging (BLI) to image and quantify non-invasively, in vitro and in vivo,intracellular metabolite fluxes and which can be applied to azido-modified compounds, such as azid ...

2014

Compact imaging system with single-photon sensitivity and picosecond time resolution for fluorescence-guided surgery with lifetime imaging capability

Claudio Bruschini, Edoardo Charbon, François Eric Powolny, Riccardo Sinisi

We present a single-photon camera for fluorescence imaging capable of providing both intensity and images, with an accuracy better than 100ps; the camera was fabricated in standard CMOS technology. As a first step towards the study of biologically relevant ...

Spie-Int Soc Optical Engineering2013