Publications de Michael Zachary Kamrath

Combining Ion Mobility and Cryogenic Spectroscopy for Structural and Analytical Studies of Biomolecular Ions

Ion mobility spectrometry (IMS) has become a valuable tool in biophysical and bioanalytical chemistry because of its ability to separate and characterize the structure of gas-phase biomolecular ions on the basis of their collisional cross section (CCS). It ...

2018

Cryogenic IR spectroscopy combined with ion mobility spectrometry for the analysis of human milk oligosaccharides

Thomas Rizzo, Michael Zachary Kamrath, Chiara Masellis, Neelam Khanal

We report here our combination of cryogenic, messenger-tagging, infrared (IR) spectroscopy with ion mobility spectrometry (IMS) and mass spectrometry (MS) as a way to identify and analyze a set of human milk oligosaccharides (HMOs) ranging from trisacchari ...

2018

A method for characterizing polysaccharides

Thomas Rizzo, Michael Zachary Kamrath, Chiara Masellis, Neelam Khanal

A method for a structural characterization of polysaccharides, comprising steps of characterizing a polysaccharide by physical measurements comprising a determination of a mass of the polysaccharide by means of mass spectrometry, a further determination of ...

2018

Glycosaminoglycan Analysis by Cryogenic Messenger-Tagging IR Spectroscopy Combined with IMS-MS

Thomas Rizzo, Michael Zachary Kamrath, Chiara Masellis, Neelam Khanal

We combine ion mobility spectrometry with cryogenic, messenger-tagging, infrared spectroscopy and mass spectrometry to . identify different isomeric disaccharides of chondroitin sulfate (CS) and heparan sulfate (HS), which are representatives of two major ...

2017

Cryogenic Vibrational Spectroscopy Provides Unique Fingerprints for Glycan Identification

Thomas Rizzo, Michael Zachary Kamrath, Chiara Masellis, Neelam Khanal

The structural characterization of glycans by mass spectrometry is particularly challenging. This is because of the high degree of isomerism in which glycans of the same mass can differ in their stereochemistry, attachment points, and degree of branching. ...

2017

Conformations of prolyl-peptide bonds in the bradykinin 1-5 fragment in solution and in the gas phase

Thomas Rizzo, Liudmila Voronina, Antoine Louis Claude Masson, Michael Zachary Kamrath, Carsten Baldauf

The dynamic nature of intrinsically disordered peptides makes them a challenge to characterize by solution-phase techniques. In order to gain insight into the relation between the disordered state and the environment, we explore the conformational space of ...

Amer Chemical Soc2016

Infrared Spectroscopy of Mobility-Selected H+-Gly-Pro-Gly-Gly (GPGG)

Thomas Rizzo, Ursula Röthlisberger, Antoine Louis Claude Masson, Michael Zachary Kamrath, Marta Andreia Da Silva Perez

We report the first results from a new instrument capable of acquiring infrared spectra of mobility-selected ions. This demonstration involves using ion mobility to first separate the protonated peptide Gly-Pro-Gly-Gly (GPGG) into two conformational famili ...

2015

Franck–Condon-like Progressions in Infrared Spectra of Biological Molecules

Thomas Rizzo, Aleksandra Zabuga, Michael Zachary Kamrath

Infrared spectra in the NH stretch region are often used for structure determination of gas-phase biological molecules. Vibrational couplings complicate the structure determination process by giving rise to additional vibrational bands along with the expec ...

American Chemical Society2015

Fragmentation mechanism of UV-excited peptides in the gas phase

Oleg Boyarkine, Thomas Rizzo, Aleksandra Zabuga, Michael Zachary Kamrath

We present evidence that following near-UV excitation, protonated tyrosine- or phenylalanine–containing peptides undergo intersystem crossing to produce a triplet species. This pathway competes with direct dissociation from the excited electronic state and ...

American Institute of Physics2014