Publications de Kei Sakamoto | EPFL Graph Search

Investigation of the specificity and mechanism of action of the ULK1/AMPK inhibitor SBI-0206965

SBI-0206965, originally identified as an inhibitor of the autophagy initiator kinase ULK1, has recently been reported as a more potent and selective AMP-activated protein kinase (AMPK) inhibitor relative to the widely used, but promiscuous inhibitor Compou ...

2021

Identification of novel PCTAIRE-1/CDK16 substrates using a chemical genetic screen

Maria Deak, Kei Sakamoto, Caterina Collodet

PCTAIRE-1 (also known as cyclin-dependent protein kinase (CDK) 16), is a Ser/Thr kinase that has been implicated in many cellular processes, including cell cycle, spermatogenesis, neurite outgrowth, and vesicle trafficking. Most recently, it has been propo ...

2019

AMPK alpha 1-LDH pathway regulates muscle stem cell self-renewal by controlling metabolic homeostasis

Kei Sakamoto, Laurent Bultot, Lin Yang, Caterina Collodet

Control of stem cell fate to either enter terminal differentiation versus returning to quiescence (self-renewal) is crucial for tissue repair. Here, we showed that AMP-activated protein kinase (AMPK), the master metabolic regulator of the cell, controls mu ...

Wiley2017

Benzimidazole derivative small-molecule 991 enhances AMPK activity and glucose uptake induced by AICAR or contraction in skeletal muscle

Maria Deak, Kei Sakamoto, Laurent Bultot, Caterina Collodet

AMP-activated protein kinase (AMPK) plays diverse roles and coordinates complex metabolic pathways for maintenance of energy homeostasis. This could be explained by the fact that AMPK exists as multiple heterotrimer complexes comprising a catalytic alpha-s ...

Amer Physiological Soc2016

Cyclin Y phosphorylation- and 14-3-3-binding-dependent activation of PCTAIRE-1/CDK16

Maria Deak, Kei Sakamoto

PCTAIRE-1 [also known as cyclin-dependent kinase 16 (CDK16)] is implicated in various physiological processes such as neurite outgrowth and vesicle trafficking; however, its molecular regulation and downstream targets are largely unknown. Cyclin Y has rece ...

Portland Press Ltd2015

Enhanced activation of cellular AMPK by dual-small molecule treatment: AICAR and A769662

Maria Deak, Kei Sakamoto, Laurent Bultot

AMP-activated protein kinase (AMPK) is a key cellular energy sensor and regulator of metabolic homeostasis. Activation of AMPK provides beneficial outcomes in fighting against metabolic disorders such as insulin resistance and type 2 diabetes. Currently, t ...

Amer Physiological Soc2014