Publications de Edward Allen Phelps

Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell

Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Petra Catalina Schwalie, Chiara Cianciaruso

Pancreatic beta cells synthesize and secrete the neurotransmitter GABA (gamma-aminobutyric acid) as a paracrine and autocrine signal to help regulate hormone secretion and islet homeostasis. Islet GABA release has classically been described as a secretory- ...

2019

Vaccine nanocarriers: Coupling intracellular pathways and cellular biodistribution to control CD4 vs CD8 T cell responses

Jeffrey Alan Hubbell, Melody Swartz, Sachiko Hirosue, Marcela Rincón-Restrepo, Sylvie Hauert, Daniel Kenneth Bonner, Edward Allen Phelps, Aaron Mayer

Nanoparticle delivery systems are known to enhance the immune response to soluble antigens (Ags) and are thus a promising tool for the development of new vaccines. Our laboratory has engineered two different nanoparticulate systems in which Ag is either en ...

Elsevier Sci Ltd2017

Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation

Jeffrey Alan Hubbell, Olivier Burri, Miriella Carmela Pasquier, Andreas Wiederkehr, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Gabriele Galliverti, Chiara Cianciaruso

A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and livecell microscopy. Guided by an observation that dispersed isle ...

Springer Nature2017

Primary human and rat beta cells release the intracellular autoantigens GAD65, IA-2 and proinsulin in exosomes together with cytokine-induced enhancers of immunity

Jeffrey Alan Hubbell, Melody Swartz, Miriella Carmela Pasquier, Davide Demurtas, Sachiko Hirosue, Romain Hamelin, Steinunn Baekkeskov Hanahan, Michele De Palma, Edward Allen Phelps, Chiara Cianciaruso

The target autoantigens in several organ-specific autoimmune diseases, including type 1 diabetes (T1D), are intracellular membrane proteins, whose initial encounter with the immune system is poorly understood. Here we propose a new model for how these prot ...

American Diabetes Association2017

Bioengineering strategies for inducing tolerance in autoimmune diabetes

Jeffrey Alan Hubbell, Steinunn Baekkeskov Hanahan, Edward Allen Phelps

Type 1 diabetes is an autoimmune disease marked by the destruction of insulin-producing beta cells in the pancreatic islets. Strategies to delay onset or prevent the autoimmune recognition of beta cell antigens or T cell-mediated killing of beta cells have ...

Elsevier Science Bv2017

Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity

Jeffrey Alan Hubbell, Miriella Carmela Pasquier, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Chiara Cianciaruso

Pancreatic islet β-cells are particularly susceptible to endoplasmic reticulum (ER) stress, which is implicated in β-cell dysfunction and loss during the pathogenesis of type 1 diabetes (T1D). The peripheral membrane protein GAD65 is an autoantigen in huma ...

Amer Diabetes Assoc2016

Compartmentalization of GABA synthesis by GAD67 differs between pancreatic beta cells and neurons

Miriella Carmela Pasquier, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Chiara Cianciaruso

The inhibitory neurotransmitter GABA is synthesized by the enzyme glutamic acid decarboxylase (GAD) in neurons and in pancreatic β-cells in islets of Langerhans where it functions as a paracrine and autocrine signaling molecule regulating the function of i ...

Public Library of Science2015

Crystalline Oligo(ethylene sulfide) Domains Define Highly Stable Supramolecular Block Copolymer Assemblies

Jeffrey Alan Hubbell, Davide Demurtas, Carrie Ellen Brubaker, Edward Allen Phelps

With proper control over copolymer design and solvation conditions, self-assembled materials display impressive morphological variety that encompasses nanoscale colloids as well as bulk three-dimensional architectures. Here we take advantage of both hydrop ...

Amer Chemical Soc2015