Publications de Anne Alison Jorstad

The effects of aging on neuropil structure in mouse somatosensory cortex-A 3D electron microscopy analysis of layer 1

Pascal Fua, Marco Cantoni, Graham Knott, Bohumil Maco, Anne Alison Jorstad, Marta Wawrzyniak

2018

NeuroMorph: A Software Toolset for 3D Analysis of Neurite Morphology and Connectivity

Graham Knott, Jérôme Blanc, Anne Alison Jorstad

The geometries of axons, dendrites and their synaptic connections provide important information about their functional properties. These can be collected directly from measurements made on serial electron microscopy images. However, manual and automated se ...

2018

Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

Graham Knott, Anne Alison Jorstad, Daniel Lebrecht

The ability to measure minute structural changes in neural circuits is essential for long-term in vivo imaging studies. Here, we propose a methodology for detection and measurement of structural changes in axonal boutons imaged with time-lapse two-photon l ...

Elife Sciences Publications Ltd2017

Delayed and Temporally Imprecise Neurotransmission in Reorganizing Cortical Microcircuits

Graham Knott, Anne Alison Jorstad, Yan Liu, Yuan Liu

Synaptic neurotransmission is modified at cortical connections throughout life. Varying the amplitude of the postsynaptic response is one mechanism that generates flexible signaling in neural circuits. The timing of the synaptic response may also play a ro ...

Soc Neuroscience2015

NeuroMorph: A Toolset for the Morphometric Analysis and Visualization of 3D Models Derived from Electron Microscopy Image Stacks

Pascal Fua, Graham Knott, Corrado Cali, Biagio Nigro, Anne Alison Jorstad, Marta Wawrzyniak

Serial electron microscopy imaging is crucial for exploring the structure of cells and tissues. The development of block face scanning electron microscopy methods and their ability to capture large image stacks, some with near isotropic voxels, is proving ...

Humana Press2015

Dense image registration and deformable surface reconstruction in presence of occlusions and minimal texture

Pascal Fua, Tien Dat Ngo, Alberto Crivellaro, Anne Alison Jorstad, Sanghyuk Park

Deformable surface tracking from monocular images is well-known to be under-constrained. Occlusions often make the task even more challenging, and can result in failure if the surface is not sufficiently textured. In this work, we explicitly address the pr ...

2015

Refining Mitochondria Segmentation in Electron Microscopy Imagery with Active Surfaces

Pascal Fua, Anne Alison Jorstad

We present an active surface-based method for refining the boundary surfaces of mitochondria segmentation data. We exploit the fact that mitochondria have thick dark membranes, so referencing the image data at the inner membrane can help drive a more accur ...

Springer2014

Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure

Pascal Fua, Graham Knott, Bohumil Maco, Anne Alison Jorstad

This protocol describes how dendrites and axons, imaged in vivo, can subsequently be analyzed in 3D using focused ion beam scanning electron microscopy (FIBSEM). The fluorescent structures are identified after chemical fixation and their position highlight ...

Elsevier Academic Press Inc2014