Publications de Jérôme Extermann

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Séverine Coquoz, David Nguyen, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Miguel Sison

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Optical projection tomography for rapid whole mouse brain imaging

Theo Lasser, Antonio Lopez, Dimitri Nestor Alice Van De Ville, Paul James Marchand, Jessica Sordet, David Nguyen, Jérôme Extermann, Miguel Sison, Marcin Antoni Sylwestrzak, Julia Sophie Katarina Nilsson, Arielle Louise Planchette

In recent years, three-dimensional mesoscopic imaging has gained significant importance in life sciences for fundamental studies at the whole-organ level. In this manuscript, we present an optical projection tomography (OPT) method designed for imaging of ...

Optical Soc Amer2017

Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Séverine Coquoz, Arno Pino Bouwens, Jérôme Extermann

Optical coherence microscopy (OCM) is an interferometric technique providing 3D images of biological samples with micrometric resolution and penetration depth of several hundreds of micrometers. OCM differs from optical coherence tomography (OCT) in that i ...

2017

3D Time-lapse Imaging and Quantification of Mitochondrial Dynamics

Theo Lasser, Antonio Lopez, Paul James Marchand, Jérôme Extermann, Miguel Sison, Amir Jean Nahas

We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopoly ...

Nature Research2017

Statistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Tristan Bolmont, David Nguyen, Vincent Khashayar Shamaei, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundre ...

Optical Soc Amer2017

NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes

Theo Lasser, Jérôme Extermann, Miguel Sison

We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT derived peptide enabling cellular uptake as well as mitochondria targeting triphenyl-phosphonium (TPP ...

Royal Soc Chemistry2017

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Anne Grapin-Botton, Corinne Berclaz, Paul James Marchand, David Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

Three dimensional time lapse imaging of live cell mitochondria with photothermal optical lock-in optical coherence microscopy (Conference Presentation)

Theo Lasser, Christophe Pache, Jérôme Extermann, Miguel Sison, Amir Jean Nahas

Spie-Int Soc Optical Engineering2016

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Harmonic Nanoparticles for Regenerative Research

Jean-Pierre Wolf, Luigi Bonacina, Jérôme Extermann

In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological ...

Journal Of Visualized Experiments2014

Harmonic Nanocrystals for Bio-Labeling: a Survey of Optical Properties and Biocompatibility

Daniel Rytz, Jean-Pierre Wolf, Sandrine Gerber, Rachid Hadji, Luigi Bonacina, Solène Marcelle Françoise Marie Passemard, Davide Städler, Jérôme Extermann

Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multi-photon microscopy. We present for the first time a complete survey of the properties of the different nanomaterials proposed to date, de ...

American Chemical Society2012

Spectral phase, amplitude, and spatial modulation from ultraviolet to infrared with a reflective MEMS pulse shaper

Nico de Rooij, Jean-Pierre Wolf, Fabio Jutzi, Wilfried Noell, Luigi Bonacina, Sébastien Lani, Jérôme Extermann

We describe the performance of a reflective pulse-shaper based on a Micro-ElectroMechanical System (MEMS) linear mirror array. It represents a substantial upgrade of a preceding release [Opt. Lett. 35, 3102 (2010)] as it allows simultaneous piston and tilt ...

Optical Society of America2011

Design, simulation, fabrication, packaging, and characterization of a MEMS-based mirror array for femtosecond pulse-shaping in phase and amplitude

Nico de Rooij, Jean-Pierre Wolf, Fabio Jutzi, Wilfried Noell, Luigi Bonacina, Sébastien Lani, Jérôme Extermann

We present an in-detail description of the design, simulation, fabrication, and packaging of a linear micromirror array specifically designed for temporal pulse shaping of ultrashort laser pulses. The innovative features of this device include a novel comb ...

American Institute of Physics2011