Publications de Ekaterina Emilova Petrova

Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals

Sebastian Maerkl, Francesca Volpetti, Ekaterina Emilova Petrova

Cell-based biosensors have great potential to detect various toxic and pathogenic contaminants in aqueous environments. However, frequently they cannot meet practical requirements due to insufficient sensing performance. To address this issue, we investiga ...

2019

Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection

Ekaterina Emilova Petrova

Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...

EPFL2018

A Microfluidic Biodisplay

Sebastian Maerkl, Francesca Volpetti, Ekaterina Emilova Petrova

Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves ...

American Chemical Society2017

Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering

Sebastian Maerkl, Matthew Christopher Blackburn, Ekaterina Emilova Petrova, Bruno Emanuel Ferreira De Sousa Correia

The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology and synthetic biology. Synthetic genes are becoming a commodity, but integrated approaches have yet to be developed that take full adva ...

Oxford Univ Press2016

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Aleksandra Radenovic, Sebastian Maerkl, Sebastian James Davis, Roman Bulushev, Ekaterina Emilova Petrova, Sanjin Marion

Through the use of optical tweezers we performed controlled translocations of DNA-protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and a dCas9 protein tailored to have five binding si ...

American Chemical Society (ACS)2016