Publications de Chiara Cianciaruso

Determinants of tumor resistance to anti-angiogenic immunotherapy in mouse models of Kras-mutant NSCLC

Ioanna Keklikoglou, Sina Nassiri, Etienne Meylan, Michele De Palma, Ece Kadioglu, Chiara Cianciaruso, Bruno Torchia, Alan Nicolas Thierry Guichard, Amaia Martinez Usatorre

AMER ASSOC CANCER RESEARCH2021

Overcoming microenvironmental resistance to PD-1 blockade in genetically engineered lung cancer models

Ioanna Keklikoglou, Nadine Fournier, Sina Nassiri, Etienne Meylan, Michele De Palma, Ece Kadioglu, Chiara Cianciaruso, Bruno Torchia, Gael Luis Boivin, Alan Nicolas Thierry Guichard, Amaia Martinez Usatorre

Immune checkpoint blockade (ICB) with PD-1 or PD-L1 antibodies has been approved for the treatment of nonsmall cell lung cancer (NSCLC). However, only a minority of patients respond, and sustained remissions are rare. Both chemotherapy and antiangiogenic d ...

AMER ASSOC ADVANCEMENT SCIENCE2021

Imaging-based spectrometer-less optofluidic biosensors based on dielectric metasurfaces for detecting extracellular vesicles

Michele De Palma, Hatice Altug, Chiara Cianciaruso, Filiz Yesilköy, Yasaman Jahani, Eduardo Romero Arvelo

Biosensors are indispensable tools for public, global, and personalized healthcare as they provide tests that can be used from early disease detection and treatment monitoring to preventing pandemics. We introduce single-wavelength imaging biosensors capab ...

Nature Research2021

All-dielectric Metasurfaces Enabling Imaging-based Real-time Biosensing

Michele De Palma, Hatice Altug, Chiara Cianciaruso, Yasaman Jahani, Eduardo Romero Arvelo

IEEE2021

Imaging-based Optofluidic Biosensors Enabled by All-dielectric metasurfaces

Michele De Palma, Hatice Altug, Chiara Cianciaruso, Yasaman Jahani, Eduardo Romero Arvelo

We present optofluidic label-free biosensors leveraging high quality-factor resonances emanated from bound-states-in-the-continuum and novel data processing. The sensors are based on imaging and offer solutions to remove sophisticated spectroscopy instrume ...

IEEE2021

Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models

Ioanna Keklikoglou, Esra Güç, Michele De Palma, Mario Leonardo Squadrito, Antonino Cassarà, Chiara Cianciaruso, Alan Nicolas Thierry Guichard

Cytotoxic chemotherapy is an effective treatment for invasive breast cancer. However, experimental studies in mice also suggest that chemotherapy has pro-metastatic effects. Primary tumours release extracellular vesicles (EVs), including exosomes, that can ...

2019

Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles

Tim Beltraminelli, Romain Hamelin, Sina Nassiri, Michele De Palma, Chiara Cianciaruso, Bruno Torchia

Extracellular vesicles (EVs), including exosomes, modulate multiple aspects of cancer biology. Tumor-associated macrophages (TAMs) secrete EVs, but their molecular features and functions are poorly characterized. Here, we report methodology for the enrichm ...

2019

Macrophage interference on chemotherapy

Sina Nassiri, Michele De Palma, Chiara Cianciaruso

NATURE PUBLISHING GROUP2019

Mechanism and effects of pulsatile GABA secretion from cytosolic pools in the human beta cell

Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Petra Catalina Schwalie, Chiara Cianciaruso

Pancreatic beta cells synthesize and secrete the neurotransmitter GABA (gamma-aminobutyric acid) as a paracrine and autocrine signal to help regulate hormone secretion and islet homeostasis. Islet GABA release has classically been described as a secretory- ...

2019

EVIR: Chimeric receptors that enhance dendritic cell cross-dressing with tumor antigens

Michele De Palma, Mario Leonardo Squadrito, Chiara Cianciaruso, Sarah Kate Hansen

We describe a lentivirus-encoded chimeric receptor, termed extracellular vesicle (EV)-internalizing receptor (EVIR), which enables the selective uptake of cancer-cell-derived EVs by dendritic cells (DCs). The EVIR enhances DC presentation of EV-associated ...

2018

Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation

Jeffrey Alan Hubbell, Olivier Burri, Miriella Carmela Pasquier, Andreas Wiederkehr, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Gabriele Galliverti, Chiara Cianciaruso

A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and livecell microscopy. Guided by an observation that dispersed isle ...

Springer Nature2017

Primary human and rat beta cells release the intracellular autoantigens GAD65, IA-2 and proinsulin in exosomes together with cytokine-induced enhancers of immunity

Jeffrey Alan Hubbell, Melody Swartz, Miriella Carmela Pasquier, Davide Demurtas, Sachiko Hirosue, Romain Hamelin, Steinunn Baekkeskov Hanahan, Michele De Palma, Edward Allen Phelps, Chiara Cianciaruso

The target autoantigens in several organ-specific autoimmune diseases, including type 1 diabetes (T1D), are intracellular membrane proteins, whose initial encounter with the immune system is poorly understood. Here we propose a new model for how these prot ...

American Diabetes Association2017

Investigating the Biological Mechanisms underlying the Initiation of Autoimmunity against Beta Cell Antigens in Type 1 Diabetes

Chiara Cianciaruso

Type 1 diabetes (T1D) is an autoimmune disease characterized by circulating autoantibodies, lymphocytic infiltration of pancreatic islets of Langerhans, and cell-specific destruction of beta cells, leading to insulin deficiency and symptomatic hyperglycemi ...

EPFL2017

Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity

Jeffrey Alan Hubbell, Miriella Carmela Pasquier, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Chiara Cianciaruso

Pancreatic islet β-cells are particularly susceptible to endoplasmic reticulum (ER) stress, which is implicated in β-cell dysfunction and loss during the pathogenesis of type 1 diabetes (T1D). The peripheral membrane protein GAD65 is an autoantigen in huma ...

Amer Diabetes Assoc2016

Compartmentalization of GABA synthesis by GAD67 differs between pancreatic beta cells and neurons

Miriella Carmela Pasquier, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Chiara Cianciaruso

The inhibitory neurotransmitter GABA is synthesized by the enzyme glutamic acid decarboxylase (GAD) in neurons and in pancreatic β-cells in islets of Langerhans where it functions as a paracrine and autocrine signaling molecule regulating the function of i ...

Public Library of Science2015

Cellular magnetic resonance with iron oxide nanoparticles: long-term persistence of SPIO signal in the CNS after transplanted cell death

Michele De Palma, Mario Leonardo Squadrito, Chiara Cianciaruso

Aim: To study the specificity of cellular MRI based on superparamagnetic iron oxide particles (SPIOs), especially within the CNS. Materials & methods: A microglial cell line was engineered for the expression of a suicide gene, the receptor of diphtheria to ...

Future Medicine Ltd2014