Publications de Kevin Keim | EPFL Graph Search

Particle-sorting fluidic device and methods for using thereof

Carlotta Guiducci, Kevin Keim, Gloria Porro

Device, system and method for sorting particles comprised into a fluid are disclosed that improve the current settings of deterministic lateral displacement (DLD)-based microfluidic devices, so to sort and detect small particles such as extracellular vesic ...

2022

Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

Bart Deplancke, Carlotta Guiducci, Kevin Keim, Pierre-Emmanuel Marie Thiriet, Gabriele Gambardella, Jörn Pezoldt

Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of ce ...

MDPI2020

In-flow measurement of cell-cell adhesion using oscillatory inertial microfluidics

Kevin Keim

Multicellular clusters in circulation can exhibit a substantially different function and biomarker significance compared to individual cells. Notably, clusters of circulating tumor cells (CTCs) are much more effective initiators of metastasis than single C ...

ROYAL SOC CHEMISTRY2020

Three-dimensional electrodes for dielectrophoretic applications

Kevin Keim

This thesis reports the use of metal-coated three-dimensional SU-8 electrodes for dielectrophoretic bio sensing and particle manipulation applications. Placing free standing three-dimensional electrodes in microfluidic channels, electric fields can be appl ...

EPFL2020

Active Posts in Deterministic Lateral Displacement Devices

Carlotta Guiducci, Kevin Keim

Using electrically connected metal-coated posts in a deterministic lateral displacement (DLD) device and applying electric fields, electrokinetics is used to tune separations, significantly decrease the critical size for separation, and increase the dynami ...

Wiley2019

On‐chip technology for single‐cell arraying, electrorotation‐based analysis and selective release

Samuel Kilchenmann, Carlotta Guiducci, Kevin Keim, Paul Karoly Otto Ery, Mohamed Zakarya Alsaid Ali Rashed

This paper reports a method for label-free single-cell biophysical analysis of multiple cells trapped in suspension by electrokinetic forces. Tri-dimensional pillar electrodes arranged along the width of a microfluidic chamber define actuators for single c ...

2019