Publications de Li Dong | EPFL Graph Search

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Thomas Lehnert, Martinus Gijs, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

Integrated Microfluidic Device for Drug Studies of Early C. Elegans Embryogenesis

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele

2018

Reversible and long-term immobilization in a hydrogel-microbead matrix for high-resolution imaging of Caenorhabditis elegans and other small organisms

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Li Dong, Govindan Narayanan Krishnamani, Jingwei Zhang

2018

An Integrated Microfluidic Device For C-Elegans Early Embryogenesis Studies And Drug Assays

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele, Jingwei Zhang

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually ...

Ieee2017

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong, Xiaopeng Huang, Jingwei Zhang

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Ieee2017

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Royal Soc Chemistry2016

Size-dependent sorting of C. elegans nematodes through an adjustable microfluidic filter structure

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

We introduce a new and simple microfluidic sorting device based on adjustable filter structures that can be easily tuned by external pressure for a specific size-dependent C. elegans worm sorting experiment. A single device can be used for selecting and so ...

2016

Versatile size-dependent sorting of C-elegans nematodes and embryos using a tunable microfluidic filter structure

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong

The roundworm Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Its life cycle consists of an embryo stage, four larval stages that can be clearly distinguis ...

Royal Soc Chemistry2016