Publications de Adrian Pascal Nievergelt

A high-bandwidth voltage amplifier for driving piezoelectric actuators in high-speed atomic force microscopy

Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Mustafa Kangül

High-speed atomic force microscopy (HS-AFM) is a technique capable of revealing the dynamics of biomolecules and living organisms at the nanoscale with a remarkable temporal resolution. The phase delay in the feedback loop dictates the achievable speed of ...

AIP Publishing2023

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture

Pierre Gönczy, Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Georgios Hatzopoulos, Niccolo Banterle, Charlène Cécile Geneviève Brillard

Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. Th ...

Nature Portfolio2021

Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface Dynamics

Aleksandra Radenovic, Daniel Constam, Georg Fantner, Adrian Pascal Nievergelt, Samuel Mendes Leitão, Alexandre Louis André Persat, Xavier Jean-Yves Pierrat, Katarina Pinjusic, Barney Frederick Drake, Vytautas Navikas, Charlène Cécile Geneviève Brillard

Nanocharacterization plays a vital role in understanding the complex nanoscale organization of cells and organelles. Understanding cellular function requires high-resolution information about how the cellular structures evolve over time. A number of techni ...

AMER CHEMICAL SOC2021

Overlapping and essential roles for molecular and mechanical mechanisms in mycobacterial cell division

John McKinney, Georg Fantner, Pascal Damian Odermatt, Adrian Pascal Nievergelt, Mélanie Thérèse Marie Hannebelle, Haig Alexander Eskandarian

Mechanisms to control cell division are essential for cell proliferation and survival. Bacterial cell growth and division require the coordinated activity of peptidoglycan synthases and hydrolytic enzymes to maintain mechanical integrity of the cell wall. ...

2020

Large‐Range HS‐AFM Imaging of DNA Self‐Assembly through In Situ Data‐Driven Control

Alireza Karimi, Georg Fantner, Adrian Pascal Nievergelt, Christoph Manuel Kammer, Maartje Martina Cornelia Bastings, Charlène Cécile Geneviève Brillard, Eva Eugene Kurisinkal

Understanding hierarchical self-assembly of biological structures requires real time measurement of the self-assembly process over a broad range of length- and timescales. The success of high-speed atomic force microscopy (HS-AFM) in imaging small scale mo ...

2019

Time-Lapse Atomic Force Microscopy Reveals New End Take Off (Neto) Dynamics in Mycobacteria

Joëlle Xiao Yuan Ven, John McKinney, Georg Fantner, Adrian Pascal Nievergelt, Mélanie Thérèse Marie Hannebelle, Haig Alexander Eskandarian, Chiara Toniolo

CELL PRESS2019

Detecting topological variations of DNA at single-molecule level

Aleksandra Radenovic, Georg Fantner, Ke Liu, Adrian Pascal Nievergelt

In addition to their use in DNA sequencing, ultrathin nanopore membranes have potential applications in detecting topological variations in deoxyribonucleic acid (DNA). This is due to the fact that when topologically edited DNA molecules, driven by electro ...

Nature Research2019

Single-molecule kinetics of pore assembly by the membrane attack complex

Georg Fantner, Adrian Pascal Nievergelt

The membrane attack complex (MAC) is a hetero-oligomeric protein assembly that kills pathogens by perforating their cell envelopes. The MAC is formed by sequential assembly of soluble complement proteins C5b, C6, C7, C8 and C9, but little is known about th ...

Springer2019

Advances in High-Speed Atomic Force Microscopy

Adrian Pascal Nievergelt

High-speed atomic force microscopy (HS-AFM) is a scanning probe technique capable of recording processes at the nanometre scale in real time. By sequentially increasing the speed of individual microscope components, images of surfaces can be recorded at up ...

EPFL2018

An analog high-speed single-cycle lock-in amplifier for next generation AFM experiments

Jens Anders, Georg Fantner, Adrian Pascal Nievergelt

In this paper, we present a new architecture for analog amplitude demodulation in high-speed atomic force microscopy (AFM) applications. It features single-cycle capabilities and the possibility to work with elevated cantilever resonance frequencies up to ...

IEEE2018

Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells

John McKinney, Georg Fantner, Adrian Pascal Nievergelt, Haig Alexander Eskandarian, Charlène Cécile Geneviève Brillard

Imaging living cells by atomic force microscopy (AFM) promises not only high-resolution topographical data, but additionally, mechanical contrast, both of which are not obtainable with other microscopy techniques. Such imaging is however challenging, as ce ...

2018

High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

Pierre Gönczy, Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Niccolo Banterle

The self-assembly of protein complexes is at the core of many fundamental biological processes1, ranging from the polymerization of cytoskeletal elements, such as microtubules2, to viral capsid formation and organelle assembly3. To reach a comprehensive un ...

2018

Digitally controlled analog proportional-integral-derivative (PID) controller for high-speed scanning probe microscopy

Santiago Harald Andany, Georg Fantner, Maja Dukic Pjanic, Nahid Hosseini, Adrian Pascal Nievergelt, Chen Yang

Nearly all scanning probe microscopes (SPMs) contain a feedback controller, which is used to move the scanner in the direction of the z-axis in order to maintain a constant setpoint based on the tip-sample interaction. The most frequently used feedback con ...

2017

Division site selection linked to inherited cell surface wave troughs in mycobacteria

Joëlle Xiao Yuan Ven, John McKinney, Neeraj Dhar, Georg Fantner, Pascal Damian Odermatt, Adrian Pascal Nievergelt, Mélanie Thérèse Marie Hannebelle, Haig Alexander Eskandarian

Cell division is tightly controlled in space and time to maintain cell size and ploidy within narrow bounds. In bacteria, the canonical Minicell (Min) and nucleoid occlusion (Noc) systems together ensure that division is restricted to midcell after complet ...

2017

Data-Driven Controller Design for Atomic-Force Microscopy

Alireza Karimi, Georg Fantner, Adrian Pascal Nievergelt, Christoph Manuel Kammer

A novel method to design data-driven, fixed-structure controllers with H2 and H∞ performance objectives is presented. The control design problem is transformed into a convex optimization problem with linear matrix inequality constraints, which can be solve ...

2017

Components for high-speed atomic force microscopy optimized for low phase-lag

Santiago Harald Andany, Georg Fantner, Jonathan David Adams, Adrian Pascal Nievergelt, Mélanie Thérèse Marie Hannebelle

IEEE2017

Method and apparatus of using a scanning probe microscope

Georg Fantner, Jonathan David Adams, Adrian Pascal Nievergelt

a scanning probe microscope for high-speed imaging and/or nanomechanical mapping. The microscope comprises a scanning probe comprising a cantilever with a tip at the distal end; and means for modulating a tip-sample distance separating the tip from an inte ...

2016

A monolithic MEMS position sensor for closed-loop high-speed atomic force microscopy

Georg Fantner, Jonathan David Adams, Nahid Hosseini, Adrian Pascal Nievergelt

The accuracy and repeatability of atomic force microscopy (AFM) imaging significantly depend on the accuracy of the piezoactuator. However, nonlinear properties of piezoactuators can distort the image, necessitating sensor-based closed-loop actuators to ac ...

2016

Harnessing the damping properties of materials for high-speed atomic force microscopy

Jürgen Brugger, Jonas Grossenbacher, Georg Fantner, Blake William Erickson, Jonathan David Adams, Adrian Pascal Nievergelt

The success of high-speed atomic force microscopy in imaging molecular motors1, enzymes2 and microbes3 in liquid environments suggests that the technique could be of significant value in a variety of areas of nanotechnology. However, the majority of atomic ...

Nature Publishing Group2016

High-Resolution Correlative Microscopy: Bridging the Gap between Single Molecule Localization Microscopy and Atomic Force Microscopy

Aleksandra Radenovic, Lely Feletti, Georg Fantner, Pascal Damian Odermatt, Arun Shivanandan, Hendrik Georges Deschout, Adrian Pascal Nievergelt, Radek Jankele

Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating ...

American Chemical Society (ACS)2015