Publications de Raphaël Etienne Jean Trouillon

Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle

Martinus Gijs, Raphaël Etienne Jean Trouillon, Martin Rainer Wohlwend

Microtubules serve as tracks for long-range intracellular trafficking of glucose transporter 4 (GLUT4), but the role of this process in skeletal muscle and insulin resistance is unclear. Here, we used fixed and live-cell imaging to study microtubule-based ...

eLIFE SCIENCES PUBL LTD2023

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Gijs, Matteo Cornaglia, Hüseyin Baris Atakan, Tunç Alkanat, Raphaël Etienne Jean Trouillon

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

A design of experiment approach for efficient multi-parametric drug testing using a Caenorhabditis elegans model

Martinus Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia, Giuseppe Tranchida

When studying the drug effectiveness towards a target model, one should distinguish the effects of the drug itself and of all the other factors that could influence the screening outcome. This comprehensive knowledge is crucial, especially when model organ ...

2018

Breast cancer HER2 analysis by extra-short incubation microfluidics-assisted fluorescence in situ hybridization (ESIMA FISH)

Martinus Gijs, Estelle Annick Cuttaz, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Laurène Nathalie Dupont

2018

Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia, Vincenzo Sorrentino

2018

Paper-Based Polymer Electrodes for Bioanalysis and Electrochemistry of Neurotransmitters

Martinus Gijs, Raphaël Etienne Jean Trouillon

2018

Microfluidic-assisted chromogenic in situ hybridization (MA-CISH) for fast and accurate breast cancer diagnosis

Martinus Gijs, Léa Suzanne Bernier, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen

Chromogenic in situ hybridization (CISH) is a common technique that is used for human epidermal growth factor receptor 2 (HER2) assessment in breast cancer diagnosis. CISH is based on the enzymatic amplification of DNA markers, generating a colorimetric im ...

Elsevier2017

Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment in breast cancer

Martinus Gijs, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Maryse Fiche, Seiya Matsuoka

Fluorescence in situ hybridization (FISH) is one of the recommended techniques for human epidermal growth factor receptor 2 (HER2) status assessment on cancer tissues. Here we develop microfluidics-assisted FISH (MA-FISH), in which hybridization of the FIS ...

Nature Publishing Group2017

A multiscale study of the role of dynamin in the regulation of glucose uptake

Martinus Gijs, Kristina Schoonjans, Johan Auwerx, Laurent Mouchiroud, Keir Joe Menzies, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia

Glucose uptake in muscle cells in response to insulin is a fundamental mechanism for metabolism. The inability of cells to mobilize the specific glucose transporter GLUT4 is believed to be at least partially accountable for diseases, like diabetes, where c ...

Royal Soc Chemistry2017

Extra short incubation microfluidic assisted – fluorescence in situ hybridization (ESIMA-FISH)

Martinus Gijs, Estelle Annick Cuttaz, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Laurène Nathalie Dupont

Fluorescence in situ hybridization (FISH) is a powerful technique for evaluating the HER2 gene status in breast cancer specimen (1). However, most of FISH assays currently used in clinical laboratories are expensive and require long experimental time, up t ...

2016

FLUORESCENCE IN SITU HYBRIDIZATION (FISH) ENHANCEMENT USING MICROFLUIDIC FLOW FOR AN ACCURATE, FAST AND ECONOMICAL ASSESSMENT OF HER2 STATUS IN BREAST CANCER

Martinus Gijs, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen, Maryse Fiche, Seiya Matsuoka

The fluorescence in situ hybridization (FISH) is the gold standard in human epidermal growth factor receptor 2 (HER2) status assessment in breast cancer. The dissemination of the technique is impeded by the cost of reagents and long experimental time. For ...

RCS Publising2016

Super-Resolution Imaging of a Dielectric Microsphere Is Governed by the Waist of Its Photonic Nanojet

Martinus Gijs, Hui Yang, Raphaël Etienne Jean Trouillon, Gergely Huszka

Dielectric microspheres with appropriate refractive index can image objects with super-resolution, that is, with a precision well beyond the classical diffraction limit. A microsphere is also known to generate upon illumination a photonic nanojet, which is ...

American Chemical Society2016

Dynamic electrochemical quantitation of dopamine release from a cells-on-paper system

Martinus Gijs, Raphaël Etienne Jean Trouillon

A hybrid microfluidic/electrochemical system is described for the time-resolved detection of dopamine (DA) from neuron-like PC12 cells cultured on a patch of filter paper. Cell adherence to the surface of the paper is investigated using fluorescence micros ...

Royal Soc Chemistry2016

Dose-response curve of a microfluidic magnetic bead-based surface coverage sandwich assay

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

Magnetic micro-and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quant ...

Elsevier2015

Interplay between the potential waveform and diffusion layer dynamics determines the time-response of voltammetric detection in microchannels

Martinus Gijs, Raphaël Etienne Jean Trouillon

The diffusion layer is a critical factor affecting the temporal response of electrochemical devices. In this article, we have investigated the effect of the relaxation of the diffusion layer during the potentiodynamic sensing of ferrocenemethanol (FcMeOH) ...

Elsevier2015

Nanocalorimetric platform for accurate thermochemical studies in microliter volumes

Thomas Lehnert, Martinus Gijs, Rima Padovani, Raphaël Etienne Jean Trouillon

A straightforward and general way for monitoring chemical reactions is via their thermal signature. Such approach requires however an experimental setup with a high thermal stability that simultaneously allows time-resolved heat detection with high sensiti ...

Royal Soc Chemistry2015

Study of constrained Brownian motion of nanoparticles near an interface using optical tweezers

Thomas Lehnert, Martinus Gijs, Hui Yang, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We demonstrate a method to determine the Brownian motion and the diffusion coefficient of a nanoparticle in water in a plane that is parallel to a solid boundary and as function of the distance normal to that boundary by using an optical tweezers instrumen ...

Spie-Int Soc Optical Engineering2015

A random-walk based model to explain ultrasensitive magnetic bead-based immunoassays

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We introduce a new modeling approach for understanding the fundamental principle of bead-based surface coverage immunoassays that rely on the specific interaction between surface-bound “small” magnetic beads (1 μm in diameter) and a flow of “large” magneti ...

2014

Cathodic pretreatment improves the resistance of boron-doped diamond electrodes to dopamine fouling

Martinus Gijs, Raphaël Etienne Jean Trouillon

The resistance of cathodically and anodically treated boron-doped diamond electrodes to dopamine fouling was investigated. It was found, using cyclic voltammetry and electrochemical impedance spectroscopy, that the cathodic preparation offers an increased ...

Elsevier2014

Magnetic Particle-Scanning for Ultrasensitive Immunodetection On-Chip

Thomas Lehnert, Martinus Gijs, Hüseyin Cumhur Tekin, Matteo Cornaglia, Raphaël Etienne Jean Trouillon

We describe the concept of magnetic particle-scanning for on-chip detection of biomolecules: a magnetic particle, carrying a low number of antigens (Ag's) (down to a single molecule), is transported by hydrodynamic forces and is subjected to successive sto ...

American Chemical Society2014