Publications de Ardemis Anoush Boghossian

Bioengineering DNA for Carbon Nanotube Sensors

Ardemis Anoush Boghossian, Yahya Rabbani

Single-walled carbon nanotubes (SWCNTs) generate near-infrared fluorescence that is useful for biomedical and environmental optical sensing. They can be colloidally suspended by π-electron-rich biopolymers like DNA that self-assemble onto their surfaces. T ...

World Scientific2025

Cation Pretreatment Enables the Saline Stability of a Near-Infrared Sensor for Dopamine

Ardemis Anoush Boghossian, Hanxuan Wang

Single-walled carbon nanotubes (SWCNTs) are wrapped with single-stranded DNA (ssDNA) to create near-infrared (NIR-II) fluorescent sensors for diverse analytes. However, the interaction between the negatively charged backbone of ssDNA and cations in biologi ...

2025

Micropreparative Gel Electrophoresis for Purification of Nanoscale Bioconjugates

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Shang-Jung Wu, Yahya Rabbani

Conventional techniques for purifying macromolecular conjugates often require complex and costly installments that are inaccessible to most laboratories. In this work, we develop a one-step micropreparative method based on a trilayered polyacrylamide gel e ...

2024

In vivo polydopamine coating of Rhodobacter sphaeroides for enhanced electron transfer

Fabian Fischer, Ardemis Anoush Boghossian, Melania Reggente

Recent advances in coupling light-harvesting microorganisms with electronic components have led to a new generation of biohybrid devices based on microbial photocatalysts. These devices are limited by the poorly conductive interface between phototrophs and ...

2024

Mind the Gap! Bridging Microbes and Electrodes through NanoBioengineering

Ardemis Anoush Boghossian

Ongoing discoveries with exoelectrogenic microbes have inspired the latest developments in whole-cell energy devices. This devicees rely on the effective interfacing of living microbes and electrodes for bioelectricity generation. A combination of material ...

FUNDACIO DE LA COMUNITAT VALENCIANA SCITO2024

Polydopamine-coated photoautotrophic bacteria for improving extracellular electron transfer in living photovoltaics

Fabian Fischer, Ardemis Anoush Boghossian, Charlotte Elisabeth Marie Roullier, Melania Reggente, Mohammed Mouhib, Patricia Brandl, Hanxuan Wang

Living photovoltaics are microbial electrochemical devices that use whole cell–electrode interactions to convert solar energy to electricity. The bottleneck in these technologies is the limited electron transfer between the microbe and the electrode surfac ...

2024

Differential near-infrared imaging of heterocysts using single-walled carbon nanotubes

Ardemis Anoush Boghossian, Alessandra Antonucci, Charlotte Elisabeth Marie Roullier, Benjamin Paul Johanès Gabriel Lambert, Alice Judith Gillen, Melania Reggente

2023

Prediction of mycotoxin response of DNA-wrapped nanotube sensor with machine learning

Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Yahya Rabbani

DNA-wrapped single-walled carbon nanotubes (DNA-SWCNTs) have demonstrated great versatility as optical sensors. SWCNTs emit a near-infrared fluorescence that is responsive to the slightest changes in their environment, enabling the creation of sensors that ...

2023

Extracellular electron transfer pathways to enhance the electroactivity of modified Escherichia coli

Ardemis Anoush Boghossian, Nils Schürgers, Melania Reggente, Mohammed Mouhib

Escherichia coli (E. coli) show limited extracellular electron transfer (EET) that compromises their use in bioelectronics. We enhance the EET in E. coli by expressing an electron transfer pathway that spans the inner and outer membranes of the cell, inclu ...

2023

Covalent conjugation of proteins onto fluorescent single-walled carbon nanotubes for biological and medical applications

Ardemis Anoush Boghossian, Hanxuan Wang

2023

Carbon nanotube uptake in cyanobacteria for near-infrared imaging and enhanced bioelectricity generation in living photovoltaics

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Alessandra Antonucci, Charlotte Elisabeth Marie Roullier, Nils Schürgers, Benjamin Paul Johanès Gabriel Lambert, Alice Judith Gillen, Melania Reggente, Mohammed Mouhib

Single-walled carbon nanotubes (SWCNTs) have unique optoelectronic properties that make them suitable for applications ranging from phototherapy to imaging and sensing, but their uptake has mainly been explored in eukaryotic cells. Here the authors explore ...

2022

Plasmon-induced near-infrared fluorescence enhancement of single-walled carbon nanotubes

Giulia Tagliabue, Amirmostafa Amirjani, Ardemis Anoush Boghossian, Sayyed Hashem Sajjadi, Shang-Jung Wu, Alessandra Antonucci, Theodoros Tsoulos

Single-walled carbon nanotubes (SWCNTs) emit near-infrared (NIR) fluorescence that is ideal for optical sensing. However, the low quantum yields diminish the sensor’s signal-to-noise ratio and limits the penetration depths for in vivo measurements. In this ...

2022

Bioengineering a glucose oxidase nanosensor for near-infrared continuous glucose monitoring

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Nils Schürgers, Hanxuan Wang

Single-walled carbon nanotubes (SWCNTs) emit photostable near-infrared fluorescence that is conducive for optical glucose monitoring. Such SWCNT-based optical sensors often require the immobilization of proteins that can confer glucose selectivity and reac ...

2022

A molecular tool and use thereof in a label-free method for detecting a target nucleic acid in a sample

Ardemis Anoush Boghossian, Alice Judith Gillen

A molecular tool and a label-free method useful for detecting a target nucleic acid in a sample are disclosed, the molecular tool comprising a substrate comprising or consisting of an optical material; a first polymeric molecule, selected from nucleic acid ...

2022

Biotechnology Applications of Nanocarbons in Plant and Algal Systems

Ardemis Anoush Boghossian, Alessandra Antonucci, Alice Judith Gillen

The unique combination of structural, optical, and electronic properties of nanocarbons (NCs) has made these materials particularly attractive for various applications in biotechnology and biomedicine, ranging from sensing, gene-delivery, bioimaging and th ...

Royal Society of Chemistry2021

Synthetic Biology: A Solution for Tackling Nanomaterial Challenges

Ardemis Anoush Boghossian, Benjamin Paul Johanès Gabriel Lambert, Alice Judith Gillen

Bioengineers have mastered practical techniques for tuning a biomaterial’s properties with only limited information on the relationship between the material’s structure and function. These techniques have been quintessential to engineering proteins, which ...

2020

Transport and programmed release of nanoscale cargo from cells by using NETosis

Daniel Meyer, Ardemis Anoush Boghossian, Alessandra Antonucci, Alice Judith Gillen

Cells can take up nanoscale materials, which has important implications for understanding cellular functions, biocompatibility as well as biomedical applications. Controlled uptake, transport and triggered release of nanoscale cargo is one of the great cha ...

ROYAL SOC CHEMISTRY2020

Sensing platform

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Nils Schürgers

Sensors comprising B-L-S; wherein: B is a biomolecule comprising one or more surface-exposed thiol moieties, L is a thiol reactive linking group, and S is a substrate; wherein L is covalently linked to B via the one or more surface-exposed thiol moieties. ...

2020

Design of Optimized PEDOT‐Based Electrodes for Enhancing Performance of Living Photovoltaics Based on Phototropic Bacteria

Ardemis Anoush Boghossian, Alessandra Antonucci, Melania Reggente, Sara Politi

Living photovoltaics represent a growing class of microbial devices that are based on whole cell–electrode interactions. The limited charge transfer at the cell–electrode interface represents a significant bottleneck in realizing an efficient technology. T ...

Wiley2020

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Vitalijs Zubkovs, Ardemis Anoush Boghossian, Shang-Jung Wu, Nils Schürgers

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

2020