Publications de Julien Hiblot | EPFL Graph Search

Recording physiological history of cells with chemical labeling

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins t ...

American Association for the Advancement of Science2024

Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag

Kai Johnsson, Julien Hiblot, Iuliia Karpenko

The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag, and CLIP-tag allow the covalent labeling of fusion proteins with synthetic molecules for applications in bioimaging and biotechnology. To guide the selection of an SLP-substrate pair and provide gui ...

AMER CHEMICAL SOC2021

Chemogenetic Control of Nanobodies

Kai Johnsson, Julien Hiblot, Helen Rebecca Ostlund Farrants

Engineered nanobody allows reversible control of activity in cells through the binding of small molecules. ...

NATURE PUBLISHING GROUP2020

Environmentally Sensitive Color-Shifting Fluorophores for Bioimaging

Kai Johnsson, Julien Hiblot

We introduce color-shifting fluorophores that reversibly switch between a green and red fluorescent form through intramolecular spirocyclization. The equilibrium of the spirocyclization is environmentally sensitive and can be directly measured by determini ...

Wiley-VCH Verlag GmbH2020

Semisynthetic sensor proteins enable metabolic assays at the point of care

Kai Johnsson, Rudolf Griss, Qiuliyang Yu, Lin Xue, Julien Hiblot

Monitoring metabolites at the point of care could improve the diagnosis and management of numerous diseases. Yet for most metabolites, such assays are not available. We introduce semisynthetic, light-emitting sensor proteins for use in paper-based metaboli ...

American Association for the Advancement of Science2018

Luciferases with tunable emission wavelengths

Kai Johnsson, Luc Reymond, Olivier Vincent Sallin, Alberto Schena, Rudolf Griss, Qiuliyang Yu, Lin Xue, Julien Hiblot, Nicholas Clarke Hill, Marina Daniela Beatrice Sabbadini

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. These tunable luciferases are generated by inserting the small luciferase NanoLuc into the structures of the self-labeling proteins SNAP-tag or HaloT ...

Wiley-Blackwell2017

Construction of pDYN6902, a new Streptomyces integrative expression vector designed for cloning sequences interfering with Escherichia coli viability

Pierre Leblond, Julien Hiblot

We describe here the construction of a new version of the Escherichia coli-Streptomyces shuttle cloning vector pIJ6902, which carries the original thiostrepton-inducible PtipA as well as the integrative and cloning systems, but with the E. coil low copy nu ...

Academic Press Inc Elsevier Science2015

Imaging and manipulating proteins in live cells through covalent labeling

Kai Johnsson, Lin Xue, Julien Hiblot, Iuliia Karpenko

The past 20 years have witnessed the advent of numerous technologies to specifically and covalently label proteins in cellulo and in vivo with synthetic probes. These technologies range from self-labeling proteins tags to non-natural amino acids, and the q ...

Nature Publishing Group2015