Publications de David William Adams

West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems

Melanie Blokesch, Sandrine Stutzmann, David William Adams, Milena Jadwiga Jaskolska, Laurie Righi, Loriane Bader, Alexandre Lemopoulos

Our understanding of the factors underlying the evolutionary success of different lineages of pandemic Vibrio cholerae remains incomplete. The West African–South American (WASA) lineage of V. cholerae, responsible for the 1991–2001 Latin American cholera e ...

Springer Science and Business Media LLC2025

Molecular mechanism of plasmid elimination by the DdmDE defense system

Melanie Blokesch, Sandrine Stutzmann, David William Adams, Laurie Righi

Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...

American Association for the Advancement of Science2024

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

Melanie Blokesch, David William Adams, Grazia Vizzarro, Alexandre Lemopoulos

In response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialized defense systems that are often clustered together on genomic islands. The O1 El Tor strains of Vibrio cholerae resp ...

American Society for Microbiology2024

Recombinant bacteria resistant to horizontal gene transfer and phage infection

Melanie Blokesch, David William Adams, Milena Jadwiga Jaskolska

The invention relates to an expression vector encoding a DNA defence system comprising polypeptides derived from Vibrio cholerae, or equivalent functional variants or homologues, conferring resistance to the maintenance of extra-genomic DNA to a recipient ...

2023

Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

Melanie Blokesch, David William Adams, Milena Jadwiga Jaskolska

Horizontal gene transfer can trigger rapid shifts in bacterial evolution. Driven by a variety of mobile genetic elements—in particular bacteriophages and plasmids—the ability to share genes within and across species underpins the exceptional adaptability o ...

2022

DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction

Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann, David William Adams

How bacteria colonize surfaces and how they distinguish the individuals around them are fundamental biological questions. Type IV pili are a widespread and multipurpose class of cell surface polymers. Here we directly visualize the DNA-uptake pilus of Vibr ...

2019

The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase

Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann, David William Adams, Jorge André Matias Pereira

Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these structures to undergo repetitive cycles of extension and retraction underpins their crucial roles in adhesion, motility and natural competence for transfor ...

2019

A benzamide-dependent ftsZ mutant reveals residues crucial for Z-ring assembly

David William Adams

In almost all bacteria, cell division is co-ordinated by the essential tubulin homologue FtsZ and represents an attractive but as yet unexploited target for new antibiotics. The benzamides, e.g. PC190723, are potent FtsZ inhibitors that have the potential ...

Wiley-Blackwell2016