Publications de Giuliana Rossi

Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution

Matthias Lütolf, Tania Hübscher, Sonja Giger, Giuliana Rossi

Gastruloids are three-dimensional embryonic organoids that reproduce key features of early mammalian development in vitro with unique scalability, accessibility, and spatiotemporal similarity to real embryos. Recently, we adapted the gastruloid culture con ...

Nature Portfolio2022

Dissecting mechanisms of chamber-specific cardiac differentiation and its perturbation following retinoic acid exposure

Matthias Lütolf, Giuliana Rossi, Nicolas Antoine Vincent Broguiere

The specification of distinct cardiac lineages occurs before chamber formation and acquisition of bona fide atrial or ventricular identity. However, the mechanisms underlying these early specification events remain poorly understood. Here, we performed sin ...

COMPANY BIOLOGISTS LTD2022

Capturing Cardiogenesis in Gastruloids

Matthias Lütolf, Romain Guiet, Mehmet Ugur Girgin, Giuliana Rossi, Andrea Boni, Nicolas Antoine Vincent Broguiere

Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed for the complex morphogenetic processes of native organogenesis. Here, we show ...

CELL PRESS2021

Polarised three-dimensional cellular aggregates

Matthias Lütolf, Giuliana Rossi

Polarised three-dimensional cellular aggregates (or gastruloids) generated in vitro from one or more pluripotent stem cells are provided. Methods for obtaining polarised three-dimensional cellular aggregates and cells (e.g. progenitor cells and derivatives ...

2020

The Transcription Factor Nfix Requires RhoA-ROCK1 Dependent Phagocytosis to Mediate Macrophage Skewing during Skeletal Muscle Regeneration

Giuliana Rossi

Macrophages (MPs) are immune cells which are crucial for tissue repair. In skeletal muscle regeneration, pro-inflammatory cells first infiltrate to promote myogenic cell proliferation, then they switch into an anti-inflammatory phenotype to sustain myogeni ...

2020

Progress and potential in organoid research

Matthias Lütolf, Andrea Manfrin, Giuliana Rossi

Tissue and organ biology are very challenging to study in mammals, and progress can be hindered, particularly in humans, by sample accessibility and ethical concerns. However, advances in stem cell culture have made it possible to derive in vitro 3D tissue ...

2018