Publications de Vytautas Navikas

Nanopore-based scanning system and method

Aleksandra Radenovic, Georg Fantner, Samuel Mendes Leitão, Vytautas Navikas

Nanopore-based scanning system including a probe structure comprising a nanopore; suction means configured to draw an end of a (bio)molecule inside the nanopore and inside the probe structure, single or multiple times; and displacement means configured to ...

2023

Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds

Aleksandra Radenovic, Georg Fantner, Samuel Mendes Leitão, Barney Frederick Drake, Sanjin Marion, Vytautas Navikas, Simon Finn Mayer, Helena Miljkovic

In current nanopore-based label-free single-molecule sensing technologies, stochastic processes influence the selection of translocating molecule, translocation rate and translocation velocity. As a result, single-molecule translocations are challenging to ...

2023

Engineering Optically Active Defects in Hexagonal Boron Nitride Using Focused Ion Beam and Water

Andras Kis, Aleksandra Radenovic, Georg Fantner, Evgenii Glushkov, Ahmet Avsar, Michal Daniel Macha, Vytautas Navikas, Aqeel Ahmed, Cheol Yeon Cheon, Nathan Cyprien Arsène Ronceray

Hexagonal boron nitride (hBN) has emerged as a promising material platform for nanophotonics and quantum sensing, hosting optically active defects with exceptional properties such as high brightness and large spectral tuning. However, precise control over ...

AMER CHEMICAL SOC2022

Experimental Combination of Super-Resolution Optical Fluctuation Imaging with Structured Illumination Microscopy for Large Fields-of-View

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Suliana Manley, Dora Mahecic, Kristin Stefanie Grussmayer, Vytautas Navikas

All fluorescence super-resolution microscopy techniques present trade-offs between, for example, resolution, acquisition speed, and live-cell compatibility. Structured illumination microscopy (SIM) improves the resolution through successive imaging of the ...

AMER CHEMICAL SOC2021

Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface Dynamics

Aleksandra Radenovic, Daniel Constam, Georg Fantner, Adrian Pascal Nievergelt, Samuel Mendes Leitão, Alexandre Louis André Persat, Xavier Jean-Yves Pierrat, Katarina Pinjusic, Barney Frederick Drake, Vytautas Navikas, Charlène Cécile Geneviève Brillard

Nanocharacterization plays a vital role in understanding the complex nanoscale organization of cells and organelles. Understanding cellular function requires high-resolution information about how the cellular structures evolve over time. A number of techni ...

AMER CHEMICAL SOC2021

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Georg Fantner, Samuel Mendes Leitão, Kristin Stefanie Grussmayer, Barney Frederick Drake, Vytautas Navikas

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

Nature Research2021

Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

Aleksandra Radenovic, Kristin Stefanie Grussmayer, Vytautas Navikas

Small-molecule fluorophores enable the observation of biomolecules in their native context with fluorescence microscopy. Specific labeling via bio-orthogonal tetrazine chemistry combines minimal label size with rapid labeling kinetics. At the same time, fl ...

2021

Biophysical applications of correlative scanning probe and super-resolution microscopy

Vytautas Navikas

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

EPFL2021

Direct Growth of Hexagonal Boron Nitride on Photonic Chips for High-Throughput Characterization

Aleksandra Radenovic, Reza Zamani, Jean Camille Benjamin Comtet, Martina Lihter, Evgenii Glushkov, Vytautas Navikas, Andrey Chernev

Adapting optical microscopy methods for nanoscale characterization of defects in two-dimensional (2D) materials is a vital step for photonic on-chip devices. To increase the analysis throughput, waveguide-based on-chip imaging platforms have been recently ...

AMER CHEMICAL SOC2021

Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer, Sanjin Marion, Vytautas Navikas

A variety of modern super-resolution micro-scopy methods provide researchers with previouslyinconceivable biological sample imaging opportunities ata molecular resolution. All of these techniques excel atimaging samples that are close to the coverslip, how ...

2021

High-Throughput Nanocapillary Filling Enabled by Microwave Radiation for Scanning Ion Conductance Microscopy Imaging

Aleksandra Radenovic, Georg Fantner, Sebastian James Davis, Samuel Mendes Leitão, Sanjin Marion, Vytautas Navikas

Solid-state nanopores provide a highly sensitive tool for single-molecule sensing and probing nanofluidic effects in solutions. Glass nanopipettes are a cheap and robust type of solid-state nanopore produced from pulling glass capillaries with opening orif ...

2020

High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Theo Lasser, Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Tomas Lukes, Vytautas Navikas

Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, ...

2020

Facile Production of Hexagonal Boron Nitride Nanoparticles by Cryogenic Exfoliation

Aleksandra Radenovic, Jean Camille Benjamin Comtet, Evgenii Glushkov, Vytautas Navikas, Andrey Chernev

Fluorescent nanoparticles with optically robust luminescence are imperative to applications in imaging and labeling. Here we demonstrate that hexagonal boron nitride (hBN) nanoparticles can be reliably produced using a scalable cryogenic exfoliation techni ...

AMER CHEMICAL SOC2019

Scanning Probe-Directed Assembly and Rapid Chemical Writing Using Nanoscopic Flow of Phospholipids

Vytautas Navikas

Nanofluidic systems offer a huge potential for discovery of new molecular transport and chemical phenomena that can be employed for future technologies. Herein, we report on the transport behavior of surface-reactive compounds in a nanometer-scale flow of ...

AMER CHEMICAL SOC2019

Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D Materials

Aleksandra Radenovic, Suliana Manley, Jean Camille Benjamin Comtet, Martina Lihter, Anna Archetti, Juan Francisco Gonzalez Marin, Evgenii Glushkov, Anton Stroganov, Vytautas Navikas, Mukeshchand Thakur

Single-molecule localization microscopy (SMLM) is a powerful tool that is routinely used for nanoscale optical imaging of biological samples. Recently, this approach has been applied to study optically active defects in two-dimensional (2D) materials. Such ...

AMER CHEMICAL SOC2019

Fluorescent Nanodiamonds as Versatile Intracellular Temperature Sensors

Aleksandra Radenovic, Evgenii Glushkov, Vytautas Navikas

Temperature is a widely known phenomenon, which plays an extremely important role in biological systems. Its behavior on the macro-scale has been quite well investigated and understood, thanks to the availability of reliable and precise thermometers such a ...

2019

Wide-Field Spectral Super-Resolution Mapping of Optically Active Defects in Hexagonal Boron Nitride

Aleksandra Radenovic, Jiandong Feng, Jean Camille Benjamin Comtet, Evgenii Glushkov, Vytautas Navikas

Point defects can have significant impact on the mechanical, electronic, and optical properties of materials. The development of robust, multidimensional, high-throughput, and large-scale characterization techniques of defects is thus crucial for the estab ...

American Chemical Society (ACS)2019

Fixed DNA Molecule Arrays for High-Throughput Single DNA-Protein Interaction Studies

Vytautas Navikas

The DNA Curtains assay is a recently developed experimental platform for protein-DNA interaction studies at the single-molecule level that is based on anchoring and alignment of DNA fragments. The DNA Curtains so far have been made by using chromium barrie ...

AMER CHEMICAL SOC2019