Publications de Saeid Ansaryan

Spatiotemporal Interrogation of Single Spheroids Using Multiplexed Nanoplasmonic-fluorescence Imaging

Matthias Lütolf, Hatice Altug, Yen-Cheng Liu, Saeid Ansaryan, Luis Francisco Lorenzo Martin

Advances in organoid models, as ex vivo mini-organs, and the development of screening imaging technologies have continuously driven each other forward. A complete understanding of organoids requires detailed insights into the intertwined intraorganoid and ...

WILEY-V C H VERLAG GMBH2025

Multimodal Nanoplasmonic and Fluorescence Imaging for Simultaneous Monitoring of Single-Cell Secretory and Intracellular Dynamics

Hatice Altug, Yen-Cheng Liu, Saeid Ansaryan

Current imaging technologies are limited in their capability to simultaneously capture intracellular and extracellular dynamics in a spatially and temporally resolved manner. This study presents a multimodal imaging system that integrates nanoplasmonic sen ...

WILEY2025

Long-Term and Continuous Plasmonic Oligonucleotide Monitoring Enabled by Regeneration Approach

Hatice Altug, Saeid Ansaryan

The demand for continuous monitoring of biochemical markers for diagnostic purposes is increasing as it overcomes the limitations of traditional intermittent measurements. This study introduces a method for long-term, continuous plasmonic biosensing of oli ...

Wiley-VCH Verlag GmbH2024

Stepwise release of Activin-A from its inhibitory prodomain is modulated by cysteines and requires furin coexpression to promote melanoma growth

Daniel Constam, Pierpaolo Ginefra, Hatice Altug, Benjamin Marcel Daniel Rothé, Céline Emmanuelle Schmuziger, Manon Bulliard, Katarina Pinjusic, Saeid Ansaryan

The Activin-A precursor dimer can be cleaved by furin, but how this proteolytic maturation is regulated in vivo and how it facilitates access to signaling receptors is unclear. Here, analysis in a syngeneic melanoma grafting model shows that without furin ...

2024

Nanoplasmonic Single-Tumoroid Microarray for Real-Time Secretion Analysis

Hatice Altug, Yen-Cheng Liu, Nicolas Antoine Vincent Broguiere, Saeid Ansaryan, Luis Francisco Lorenzo Martin

Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine as such 3D structures grown from tumor cells recapitulate better tumor characteristics, making these tumoroids unique for personalized cancer research. Assessment o ...

2024

High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays

Hatice Altug, Yen-Cheng Liu, Saeid Ansaryan

Methods for the analysis of cell secretions at the single-cell level only provide semiquantitative endpoint readouts. Here we describe a microwell array for the real-time spatiotemporal monitoring of extracellular secretions from hundreds of single cells i ...

NATURE PORTFOLIO2023

Real-time monitoring of single-cell secretion with a high-throughput nanoplasmonic microarray

Hatice Altug, Xiaokang Li, Yen-Cheng Liu, Eduardo Romero Arvelo, Saeid Ansaryan

Proteins secreted by cells play significant roles in mediating many physiological, developmental, and pathological processes due to their functions in intra/intercellular communication and signaling. Conventional end-point methods are insufficient for unde ...

Elsevier2022