Publications de Guido Van Mierlo

Engineering next-generation microfluidic technologies for single-cell phenomics

Bart Deplancke, Johannes Julius Bues, Guido Van Mierlo, Camille Lucie Germaine Lambert, Orane Josiane Guillaume-Gentil

Springer Science and Business Media LLC2025

Context transcription factors establish cooperative environments and mediate enhancer communication

Bart Deplancke, Julie Marie Russeil, Vincent Roland Julien Gardeux, Judith Franziska Kribelbauer, Guido Van Mierlo

Many enhancers control gene expression by assembling regulatory factor clusters, also referred to as condensates. This process is vital for facilitating enhancer communication and establishing cellular identity. However, how DNA sequence and transcription ...

2024

Non-coding variants impact cis-regulatory coordination in a cell type-specific manner

Bart Deplancke, Vincent Roland Julien Gardeux, Judith Franziska Kribelbauer, Guido Van Mierlo

Background: Interactions among cis-regulatory elements (CREs) play a crucial role in gene regulation. Various approaches have been developed to map these interactions genome-wide, including those relying on interindividual epigenomic variation to identify ...

2024

The evolution of DNA sequencing with microfluidics

Bart Deplancke, Johannes Julius Bues, Guido Van Mierlo, Camille Lucie Germaine Lambert

The adoption of microfluidics was fundamental to the development of cost-effective, high-throughput DNA sequencing. As the field progresses towards multi-omics, we reflect on the key concepts underlying microfluidics and how resulting engineering advances ...

NATURE PORTFOLIO2024

A human omentum-specific mesothelial-like stromal population inhibits adipogenesis through IGFBP2 secretion

Bart Deplancke, Pernille Yde Rainer, Radiana Ferrero, Julie Marie Russeil, Magda Zachara, Daniel Alpern, Vincent Roland Julien Gardeux, Jörn Pezoldt, Guido Van Mierlo

Although several mouse and human adipose SVF cellular subpopulations have by now been identified, we still lack an understanding of the cellular and functional variability of adipose stem and progenitor cell (ASPC) populations across human fat depots. To a ...

Cell Press2024

Chromatin modules and their implication in genomic organization and gene regulation

Bart Deplancke, Judith Franziska Kribelbauer, Guido Van Mierlo

Regulation of gene expression is a complex but highly guided process. While genomic technologies and computational approaches have allowed high-throughput mapping of cis-regulatory elements (CREs) and their interactions in 3D, their precise role in regulat ...

ELSEVIER SCIENCE LONDON2023

Direct In Vivo Activation of T Cells with Nanosized Immunofilaments Inhibits Tumor Growth and Metastasis

Bart Deplancke, Jorieke Weiden, Guido Van Mierlo

Adoptive T cell therapyhas successfully been implementedfor thetreatment of cancer. Nevertheless, ex vivo expansion of T cells byartificial antigen-presenting cells (aAPCs) remains cumbersome andcan compromise T cell functionality, thereby limiting their t ...

AMER CHEMICAL SOC2023

Postnatal expansion of mesenteric lymph node stromal cells towards reticular and CD34(+) stromal cell subsets

Bart Deplancke, Julie Marie Russeil, Wanze Chen, Vincent Roland Julien Gardeux, Maria Litovchenko, Marjan Biocanin, Jörn Pezoldt, Guido Van Mierlo

Gut-draining mesenteric lymph nodes (LN) provide the framework to shape intestinal adaptive immune responses. Based on the transcriptional signatures established by our previous work, the composition and immunomodulatory function of LN stromal cells (SC) v ...

Nature Portfolio2022

Chromatin Proteomics to Study Epigenetics - Challenges and Opportunities

Guido Van Mierlo

Regulation of gene expression is essential for the functioning of all eukaryotic organisms. Understanding gene expression regulation requires determining which proteins interact with regulatory elements in chromatin. MS-based analysis of chromatin has emer ...

AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC2021

Identification of Allobaculum mucolyticum as a novel human intestinal mucin degrader

Guido Van Mierlo

The human gut microbiota plays a central role in intestinal health and disease. Yet, many of its bacterial constituents are functionally still largely unexplored. A crucial prerequisite for bacterial survival and proliferation is the creation and/or exploi ...

2021