Publications associées à Fluidic microstructuring of alginate hydrogels for the single cell niche

Engineering hydrogel microenvironments for epithelial organoid culture

Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...

EPFL2024

Deciphering the nature of cell state transitions in single cells using quantitative modeling of temporal dynamics

Alex Russell Lederer

Cells are the smallest operational units of living systems. Through synthesis of various biomolecules and exchange of signals with the environment, cells tightly regulate their composition to realize a specific functional state. The transformation of a cel ...

EPFL2024

Unveiling breast cancer metastasis through an advanced X-ray imaging approach

Marianne Liebi

Breast cancer is a significant global health burden, causing a substantial number of deaths. Systemic metastatic tumour cell dissemination is a major cause of poor outcomes. Understanding the mechanisms underlying metastasis is crucial for effective interv ...

Nature Portfolio2024

Disruption of stem cell niche-confined R-spondin 3 expression leads to impaired hematopoiesis

Freddy Radtke, Ute Koch, Jialin Zhang, Soufiane Boumahdi

Self-renewal and differentiation of stem and progenitor cells are tightly regulated to ensure tissue homeostasis. This regulation is enabled both remotely by systemic circulating cues, such as cytokines and hormones, and locally by various niche-confined f ...

ELSEVIER2023

III-V Nitride Semiconductors Deposited At Low Temperature For Photovoltaic Applications

Jonathan Emanuel Thomet

This thesis reports on the study and use of low temperature processes for the deposition of indium gallium nitride (InGaN) thin films in order to alleviate some of the present drawbacks of its monolitic deposition on silicon for photovoltaic applications. ...

EPFL2023

Plasma-Assisted Hybrid Vapour Deposition Technology for Thin Film Fabrication

Caroline Hain

Continuous development of thin film deposition technologies is essential for the fabrication of films that meet the specific requirements of their target applications and working conditions. Therefore, it is necessary to increase the number of accessible a ...

EPFL2023

Actuated 3D microgels for single cell mechanobiology

Christina Myra Tringides, Mahmut Selman Sakar, Ece Özelçi, Berna Ayse Özkale Edelmann

We present a new cell culture technology for large-scale mechanobiology studies capable of generating and applying optically controlled uniform compression on single cells in 3D. Mesenchymal stem cells (MSCs) are individually encapsulated inside an optical ...

ROYAL SOC CHEMISTRY2022

The matricellular protein Drosophila Cellular Communication Network Factor is required for synaptic transmission and female fertility

Evelyne Ruchti, Brian Donal McCabe

Within the extracellular matrix, matricellular proteins are dynamically expressed nonstructural proteins that interact with cell surface receptors, growth factors, and proteases, as well as with structural matrix proteins. The cellular communication networ ...

Genetics Society America2022

Investigation in Crystal Growth/Morphology and Interface Engineering of Perovskite Solar Cells by Different Deposition Methods

Nadja Isabelle Desiree Klipfel

A new and promising technology has emerged to compete with traditional silicon photovoltaic semiconductors. The low material and processing costs and high-power conversion efficiencies of organic-inorganic metal halide perovskites make these a promising te ...

EPFL2022

Generation of a Gal4-dependent gene recombination and illuminating mouse

Freddy Radtke

Cell labeling technologies, including the Cre/loxP system, are powerful tools in developmental biology. Although the conventional Cre/loxP system has been extensively used to label the expression of specific genes, it is less frequently used for labeling p ...

INT PRESS EDITING CENTRE INC2022

Immunosuppressive roles of Activin-A in melanoma and characterization of its precursor cleavage

Katarina Pinjusic

Activin-A, a transforming growth factor ê µ family member, is a pleiotropic cytokine with diverse functions in development, fertility, adult tissue homeostasis, and aging. Accordingly, deregulation of Activin-A signaling has been associated with many patho ...

EPFL2022

Defined matrices for epithelial organoid culture and disease modelling

Saba Rezakhani

Advances in our understanding of the biology of stem cells and their niches have provided the necessary foundation to create in vitro complex self-organizing three-dimensional (3D) structures called organoids. While organoids represent a highly promising n ...

EPFL2021

Extracellular matrix requirements for gastrointestinal organoid cultures

Organoids are a new class of biological model systems that have garnered significant interest in the life sciences. When provided with the proper 3D matrix and biochemical factors, stem cells can self-organize and form tissue-specific organoids. Thus far, ...

2021

Developing new tools to study and program cell fate at the single-cell level

Marjan Biocanin

Single-cell transcriptomics (scRNA-seq) started a technological revolution in biology by enabling through the plethora of methods to assess a molecular state of the cell on systems level without the strict necessity of the prior knowledge of the cell state ...

EPFL2021

Vapor Transport Deposition of Methylammonium Iodide for Perovskite Solar Cells

Christophe Ballif, Quentin Thomas Jeangros, Cédric Bucher, Florent Sahli, Léo Duchêne, Björn Niesen, Aymeric Michel Schafflützel, Nathanaël Miaz, Quentin Jean-Marie Armand Guesnay, Niccolo Salsi

Vapor-based processes are promising options to deposit metal halide perovskite solar cells in an industrial environment due to their ability to deposit uniform layers over large areas in a controlled environment without resorting to the use of (possibly to ...

2021

Metabolic and transcriptomic profiles of glioblastoma invasion revealed by comparisons between patients and corresponding orthotopic xenografts in mice

Lijing Xin, Cristina Ramona Cudalbu, Mario Gaetano Lepore, Marta Lai

The invasive behavior of glioblastoma, the most aggressive primary brain tumor, is considered highly relevant for tumor recurrence. However, the invasion zone is difficult to visualize by Magnetic Resonance Imaging (MRI) and is protected by the blood brain ...

2021

Mechanical Properties of Soft Biological Membranes for Organ-on-a-Chip Assessed by Bulge Test and AFM

Johann Michler, Georg Fantner, Veronika Cencen

Advanced in vitro models called “organ-on-a-chip” can mimic the specific cellular environment found in various tissues. Many of these models include a thin, sometimes flexible, membrane aimed at mimicking the extracellular matrix (ECM) scaffold of in vivo ...

2021

Vapor deposition of metal halide perovskite thin films: Process control strategies to shape layer properties

Christophe Ballif, Quentin Thomas Jeangros, Florent Sahli, Quentin Jean-Marie Armand Guesnay

Vapor-based processes are particularly promising to deposit the perovskite thin film absorber of solar cells. These deposition methods are up-scalable, involve a controlled solvent-free environment, have the ability to conformally coat rough substrates, in ...

2021

Wireless soft microscale actuators and robotic devices to study mechanobiology

Raquel Filipa Penacho Parreira

Tissues morphogenesis and homeostasis involve the spatiotemporal regulation of mechanics at multiple scales. Characterization of mechanical properties of biological systems as well as investigating the effects of mechanical forces on biological function ar ...

EPFL2021

Co-regulation of endothelial and myogenic cell fates through intercellular crosstalk in skeletal muscle

Umji Lee

Skeletal muscle is one of the most dynamic organs in the human body, which has a vigorous potential to regenerate and adapt to environmental changes. Depending on environments, skeletal muscle enables essential life activities to survive by controlling mov ...

EPFL2021