Publications associées à Reaction wavefront theory of notochord segment patterning

How are cells instructed to form somite boundaries by the zebrafish segmentation clock?

In vertebrate embryos, the elongating body axis is patterned via the sequential and rhyth-mic production of segments from a posterior unsegmented tissue called the presomitic mesoderm (PSM). This process is controlled by a population of cellular oscillator ...

EPFL2023

Development of a Robotic Microsurgeon for Embryonic Research

Ece Özelçi

Understanding how biological matter takes its shape is instrumental to biology, bioengineering, medicine, and bioinspired engineering. Gaining information on the principles of morphogenesis could enable clinicians to correct developmental abnormalities, ev ...

EPFL2023

Preparation of Single-Somite Explants from Zebrafish Embryos

Andrew Charles Oates, Sundar Ram Naganathan

The body axis of vertebrate embryos is periodically subdivided into 3D multicellular units called somites. While genetic oscillations and molecular prepatterns determine the initial length-scale of somites, mechanical processes have been implicated in sett ...

JOURNAL OF VISUALIZED EXPERIMENTS2022

Hunting for the wavefront: investigation of somite boundary positioning in zebrafish

Arianne Bercowsky Rama

Somitogenesis is the rhythmic and sequential formation of somites, which are tissue blocks that give rise to segmented adult body structures including the vertebrae and associated muscle. Somite formation is controlled by the segmentation clock, a populati ...

EPFL2022

In vitro endoderm emergence and self-organisation in the absence of extraembryonic tissues and embryonic architecture

Stefano Davide Vianello

In humans, mice, and other mammals key internal organs such as the gut, the lungs, the pancreas, and the liver all derive from the same embryonic tissue: the endoderm. The development of all of these structures thus depends on a same set of early cells, an ...

EPFL2022

Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation

Matthias Rüegg, Mahmut Selman Sakar, Erik Mailand, Ece Özelçi, Andrew Charles Oates

Classic microsurgical techniques, such as those used in the early 1900s by Mangold and Spemann, have been instrumental in advancing our understanding of embryonic development. However, these techniques are highly specialized, leading to issues of inter-ope ...

Nature Research2022

Targeted search for scaling genes reveals matrix metalloproteinase 3 as a scaler of the dorsal-ventral pattern in Xenopus laevis embryos

Daria Korotkova

How embryos scale patterning according to size is still not fully understood. Through in silico screening and analysis of reaction-diffusion systems that could be responsible for scaling, we predicted the existence of genes whose expression is sensitive to ...

Cell Press - Elsevier2022

Towards engineering an artificial neural tube

Jisoo Park

In this thesis, I present a self-organizing neural tube organoid that is strikingly similar in morphology, cell-type composition, and patterning to the mouse embryonic neural tube. When exposed to a sequence of epiblast culture conditions and neural differ ...

EPFL2021

Patterning and mechanics of somite boundaries in zebrafish embryos

Andrew Charles Oates, Sundar Ram Naganathan

The body axis of vertebrates is subdivided into repetitive compartments called somites, which give rise primarily to the segmented architecture of the musculoskeletal system in the adult body. Somites form in a sequential and rhythmic manner in embryos and ...

2020

In vitro modeling of early mouse development

Mehmet Ugur Girgin

Previous attempts to recapitulate embryogenesis in a developmentally relevant context started with aggregates composed of a few thousand ESCs, termed embryoid bodies (EB), that upon induction of differentiation reveal a surprising level of autonomous cell ...

EPFL2020

What are you synching about? Emerging complexity of Notch signaling in the segmentation clock

Olivier François Venzin, Andrew Charles Oates

The Segmentation clock is a population of cellular genetic oscillators, located in the posterior of the elongating vertebrate embryo, that governs the rhythmic and sequential segmentation of the body axis into somites. Somites are blocks of cells that give ...

ACADEMIC PRESS INC ELSEVIER SCIENCE2020

Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells

Matthias Lütolf, Felix Naef, Eric Paquet, Yoji Tabata, Ambroise Roger Vuaridel, François Réal Rivest, Andrea Manfrin

Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...

NATURE PUBLISHING GROUP2019

The mouse genome displays highly dynamic populations of KRAB-zinc finger protein genes and related genetic units

Didier Trono, Annamaria Kauzlaric, Julien Léonard Duc, Gabriela Ecco, Marco Cassano, Michaël Imbeault

KRAB-containing poly-zinc finger proteins (KZFPs) constitute the largest family of transcription factors encoded by mammalian genomes, and growing evidence indicates that they fulfill functions critical to both embryonic development and maintenance of adul ...

Public Library of Science2017

Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells

Denis Duboule, Patrick Schorderet, Guillaume Andrey, Nayuta Yakushiji

During embryonic development, fields of progenitor cells form complex structures through dynamic interactions with external signaling molecules. How complex signaling inputs are integrated to yield appropriate gene expression responses is poorly understood ...

National Academy of Sciences2017

Faster embryonic segmentation through elevated Delta-Notch signalling

Andrew Charles Oates

An important step in understanding biological rhythms is the control of period. A multicellular, rhythmic patterning system termed the segmentation clock is thought to govern the sequential production of the vertebrate embryo's body segments, the somites. ...

Nature Publishing Group2016

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation

Daniel Alpern

TAF4 (TATA-binding protein-associated factor 4) and its paralogue TAF4b are components of the TFIID core module. We inactivated the murine Taf4a gene to address Taf4 function during embryogenesis. Here we show that Taf4a(-/-) embryos survive until E9.5 whe ...

Nature Publishing Group2016

Tetrapod axial evolution and developmental constraints; Empirical underpinning by a mouse model

Denis Duboule

The tetrapod vertebral column has become increasingly complex during evolution as an adaptation to a terrestrial life. At the same time, the evolution of the vertebral formula became subject to developmental constraints acting on the size of the cervical a ...

Elsevier2015

Regulatory Switches Underlie Hoxd Gene Activation in Tetrapod Limbs

Guillaume Andrey

Accurate spatio-temporal control of Hox cluster genes expression is critical for the proper organization of body structures during vertebrate embryogenesis. In the limbs, Hoxd genes are critical in setting up the proximodistal and anteroposterior axes. Sev ...

EPFL2013

A Subpopulation of Smooth Muscle Cells, Derived from Melanocyte-Competent Precursors, Prevents Patent Ductus Arteriosus

Friedrich Beermann

Background: Patent ductus arteriosus is a life-threatening condition frequent in premature newborns but also present in some term infants. Current mouse models of this malformation generally lead to perinatal death, not reproducing the full phenotypic spec ...

Public Library of Science2013

Dual Lineage-Specific Expression of Sox17 During Mouse Embryogenesis

Anne Grapin-Botton, Marine Marie Kraus, Laurence Anne E Lemaire

Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-exp ...

Wiley-Blackwell2012