Publications associées à Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle

Towards understanding centriole elimination

Centrioles are microtubule-based structures crucial for forming flagella, cilia and centrosomes. Through these roles, centrioles are critical notably for proper cell motility, signalling and division. Recent years have advanced significantly our understand ...

Royal Soc2023

Extensive programmed centriole elimination unveiled in C. elegans embryos

Pierre Gönczy, Nils Kalbfuss

Centrioles are critical for fundamental cellular processes, including signaling, motility, and division. The extent to which centrioles are present after cell cycle exit in a developing organism is not known. The stereotypical lineage of Caenorhabditis ele ...

2023

Centromere structure and function: lessons from Drosophila

Eftychia Kyriacou

The fruit fly Drosophila melanogaster serves as a powerful model organism for advancing our understanding of biological processes, not just by studying its similarities with other organisms including ourselves but also by investigating its differences to u ...

Genetics Society America2023

Primary data for EPFL thesis "Characterisation of conditional alleles of the Schizosaccharomyces pombe cytokinesis regulator byr4"

Michael Grüner Vindfeldt

Regulation of cytokinesis is essential for the cell during its division cycle. Failure to do so can lead to aneuploidy, which can be fatal and lead to senescence or cancer. A useful model organism for studying cytokinesis in eukaryotes is Schizosaccharomyc ...

EPFL Infoscience2023

Characterisation of conditional alleles of the Schizosaccharomyces pombe cytokinesis regulator byr4

Michael Grüner Vindfeldt

Regulation of cytokinesis is essential for the cell during its division cycle. Failure to do so can lead to aneuploidy, which can be fatal and lead to senescence or cancer. A useful model organism for studying cytokinesis in eukaryotes is Schizosaccharomyc ...

EPFL2023

CENP-A and CENP-B collaborate to create an open centromeric chromatin state

Beat Fierz, Harsh Nagpal, Wei Cai

Centromeres are epigenetically defined via the presence of the histone H3 variant CENP-A. Contacting CENP-A nucleosomes, the constitutive centromere associated network (CCAN) and the kinetochore assemble, connecting the centromere to spindle microtubules d ...

Nature Portfolio2023

Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1

Pierre Gönczy, Mary-Claude Croisier-Coeytaux, Coralie Busso, Tamara Mikeladze-Dvali, Marie Juliette Ségolène Pierron, Keshav Jha, Alexander Woglar

In most metazoans, centrioles are lost during oogenesis, ensuring that the zygote is endowed with the correct number of two centrioles, which are paternally contributed. How centriole architecture is dismantled during oogenesis is not understood. Here, we ...

Wiley2023

Dissecting centriole fate in C. elegans development

Nils Kalbfuss

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel

Pierre Gönczy, Georgios Hatzopoulos

Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9 fold radial symmetry imparted by a ...

CELL PRESS2022

Principles of checkpoint override

Ahmad Sadeghi

Why biological quality-control systems fail is often mysterious. Specifically, checkpoints such as the DNA damage checkpoint or the spindle assembly checkpoint are overriden after prolonged arrests allowing cells to continue dividing despite the continued ...

EPFL2022

Metabolic regulation of cytokinesis in Schizosaccharomyces pombe

Özge Uysal Özdemir

All cells need to be able to sense their environment, and adapt their metabolism, growth and cell division appropriately. In this study, I examined the response of Schizosaccharomyces pombe to a change in the carbon source and how that affects cytokinesis. ...

EPFL2022

Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8

Eftychia Kyriacou

Meiotic processes are potentially dangerous to genome stability and could be disastrous if activated in proliferative cells. Here we show that two key meiosis-defining proteins, the topoisomerase Spo11 (which forms double-strand breaks) and the meiotic coh ...

NATURE RESEARCH2021

Pulchelloid A, a sesquiterpene lactone from the Canadian prairie plant Gaillardia aristata inhibits mitosis in human cells

Tatiana Favez, Pierre Gönczy, Georgios Hatzopoulos

Background The Canadian prairie ecosystem presents a rich source of natural products from plants that are subjected to herbivory by grazing mammals. This type of ecological competition may contribute to the production of natural products of interest in cel ...

SPRINGER2021

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin

Pierre Gönczy, Coralie Busso, Fernando Romero Balestra, Benita Wolf, Tessa Vivianne Averink, Alizée Buff

TRIM37 is an E3 ubiquitin ligase mutated in Mulibrey nanism, a disease with impaired organ growth and increased tumor formation. TRIM37 depletion from tissue culture cells results in supernumerary foci bearing the centriolar protein Centrin. Here, we chara ...

2021

A helical inner scaffold provides a structural basis for centriole cohesion

Henning Paul-Julius Stahlberg, Virginie Hamel, Paul Guichard, Davide Gambarotto

The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary ...

2020

Centriole foci persist in starfish oocytes despite Polo-like kinase 1 inactivation or loss of microtubule nucleation activity

Pierre Gönczy, Marie Juliette Ségolène Pierron, Nils Kalbfuss

Centrioles must be eliminated or inactivated from the oocyte to ensure that only the two functional centrioles contributed by the sperm are present in the zygote. Such removal can occur during oogenesis, as in Drosophila, where departure of Polo kinase fro ...

2020

A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division

Christina Ernst

Genome stability relies on proper coordination of mitosis and cytokinesis, where dynamic microtubules capture and faithfully segregate chromosomes into daughter cells. With a high-content RNAi imaging screen targeting more than 2,000 human lncRNAs, we iden ...

Nature Research2020

Torsin ATPases influence chromatin interaction of the Torsin regulator LAP1

Rudolf Hovius, Beat Fierz

The inner nuclear membrane is functionalized by diverse transmembrane proteins that associate with nuclear lamins and/or chromatin. When cells enter mitosis, membrane-chromatin contacts must be broken to allow for proper chromosome segregation; yet how thi ...

2020

Spatial control of nucleoporin condensation by fragile X-related proteins

Alessandro Berto

Nucleoporins (Nups) build highly organized nuclear pore complexes (NPCs) at the nuclear envelope (NE). Several Nups assemble into a sieve-like hydrogel within the central channel of the NPCs. In the cytoplasm, the soluble Nups exist, but how their assembly ...

WILEY2020

Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

Oriented cell division is a fundamental mechanism to control asymmetric stem cell division, neural tube elongation and body axis extension, among other processes. During zebrafish gastrulation, when the body axis extends, dorsal epiblast cells display divi ...

Nature Publishing Group2020