Publication

Microfluidics-generated immobilized biomolecule gradients on hydrogels

Matthias Lütolf, Stefan Kobel, Steffen Cosson
2008
Conference Papers

Résumé

Extra-cellular matrix (ECM) tethered morphogen gradients play an important role in tissue development and regeneration. There is currently only a limited set of model systems which can mimic these gradients in an artificial ECM-like environment. We have developed such a system that is able to stably immobilize a microfluidics-generated protein gradient on a poly(ethylene) glycol (PEG) hydrogel. After patterning, the hydrogel can be released from the chip and be directly used for cell culture.

Source officielle

https://infoscience.epfl.ch/entities/publication/c80c9b67-8250-4e1d-8c5f-99c2085d25f1

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Matthias Lütolf, Stefan Kobel, Steffen Cosson
2008
Conference Papers

Résumé

Source officielle

https://infoscience.epfl.ch/entities/publication/c80c9b67-8250-4e1d-8c5f-99c2085d25f1

À propos de ce résultat

Concepts associés (13)

Culture cellulaire

La culture cellulaire est un ensemble de techniques de biologie utilisées pour faire croître des cellules hors de leur organisme (ex-vivo) ou de leur milieu d'origine, dans un but d'expérimentation scientifique ou de fécondation in vitro. Les cellules mises en culture peuvent être: des micro-organismes libres (bactéries ou levures) des cellules « saines » prélevées fraîchement d'un organisme (biopsie...), on parle alors de « culture primaire ».

Culture tissulaire

vignette|Flacons contenant un milieu de culture de culture tissulaire qui fournit la nourriture nécessaire à la croissance des cellules. La culture tissulaire est la croissance de tissus ou de cellules dans un milieu artificiel séparé de l'organisme parent. Cette technique est également appelée micropropagation. Elle est généralement facilitée par l'utilisation d'un milieu de culture liquide, semi-solide ou solide, tel que le bouillon ou la gélose.

Primary cell culture

Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines. In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). However, primary cells require adequate substrate and nutrient conditions to thrive and after a certain number of divisions they acquire a senescent phenotype, leading to irreversible cell cycle arrest.

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Next-Generation Modeling of Cancer Using Organoids

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In the last decade, organoid technology has become a cornerstone in cancer research. Organoids are long-term primary cell cultures, usually of epithelial origin, grown in a three-dimensional (3D) protein matrix and a fully defined medium. Organoids can be ...

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