Publications associées à Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1

Pierre Gönczy, Mary-Claude Croisier-Coeytaux, Coralie Busso, Tamara Mikeladze-Dvali, Marie Juliette Ségolène Pierron, Keshav Jha, Alexander Woglar

In most metazoans, centrioles are lost during oogenesis, ensuring that the zygote is endowed with the correct number of two centrioles, which are paternally contributed. How centriole architecture is dismantled during oogenesis is not understood. Here, we ...

Wiley2023

Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle

Martinus Gijs, Raphaël Etienne Jean Trouillon, Martin Rainer Wohlwend

Microtubules serve as tracks for long-range intracellular trafficking of glucose transporter 4 (GLUT4), but the role of this process in skeletal muscle and insulin resistance is unclear. Here, we used fixed and live-cell imaging to study microtubule-based ...

eLIFE SCIENCES PUBL LTD2023

Sperm-contributed centrioles segregate stochastically into blastomeres of 4-cell stage Caenorhabditis elegans embryos

Pierre Gönczy, Fernando Romero Balestra

Whereas both sperm and egg contribute nuclear genetic material to the zygote in metazoan organisms, the inheritance of other cellular constituents is unequal between the 2 gametes. Thus, 2 copies of the centriole are contributed solely by the sperm to the ...

GENETICS SOCIETY AMERICA2023

Dissecting centriole fate in C. elegans development

Nils Kalbfuss

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

Structures of SAS-6 coiled coil hold implications for the polarity of the centriolar cartwheel

Pierre Gönczy, Georgios Hatzopoulos

Centrioles are eukaryotic organelles that template the formation of cilia and flagella, as well as organize the microtubule network and the mitotic spindle in animal cells. Centrioles have proximal-distal polarity and a 9 fold radial symmetry imparted by a ...

CELL PRESS2022

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Tatiana Favez, Pierre Gönczy, Virginie Hamel, Santiago Harald Andany, Georg Fantner, Oliver Hantschel, Georgios Hatzopoulos, Niccolo Banterle

Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...

Nature Research2021

A helical inner scaffold provides a structural basis for centriole cohesion

Henning Paul-Julius Stahlberg, Virginie Hamel, Paul Guichard, Davide Gambarotto

The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary ...

2020

Architecture of the centriole cartwheel‐containing region revealed by cryo‐electron tomography

Henning Paul-Julius Stahlberg, Virginie Hamel, Paul Guichard

Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture ...

2020

Centriole foci persist in starfish oocytes despite Polo-like kinase 1 inactivation or loss of microtubule nucleation activity

Pierre Gönczy, Marie Juliette Ségolène Pierron, Nils Kalbfuss

Centrioles must be eliminated or inactivated from the oocyte to ensure that only the two functional centrioles contributed by the sperm are present in the zygote. Such removal can occur during oogenesis, as in Drosophila, where departure of Polo kinase fro ...

2020

Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Pierre Gönczy, Davide Demurtas, Alexandra Bezler, Sergey Nazarov, Paul Guichard, Veronika Nemcíková Villímová, Georgios Hatzopoulos

Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...

2020

Centriole assembly at a glance

Pierre Gönczy, Georgios Hatzopoulos

The centriole organelle consists of microtubules (MTs) that exhibit a striking 9-fold radial symmetry. Centrioles play fundamental roles across eukaryotes, notably in cell signaling, motility and division. In this Cell Science at a Glance article and accom ...

2019

Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails

Davide Demurtas, Virginie Hamel, Paul Guichard

Microtubule doublets (MTDs), consisting of an incomplete B-microtubule at the surface of a complete A-microtubule, provide a structural scaffold mediating intraflagellar transport and ciliary beating. Despite the fundamental role of MTDs, the molecular mec ...

American Association for the Advancement of Science2019

Protein Engineering Tools to Explore the Function of Protein Post-Translational Modifications for Chromatin and Microtubule Cytoskeletal Biology

Ninad Dilip Agashe

Protein post-translational modifications (PTMs) play a crucial role in expanding the protein diversity and are one of the major mechanisms through which cells respond to ever changing environmental cues. The function of two of the most important cellular c ...

EPFL2019

Integrative approach to analyze the proximal region of the Trichonympha agilis centriole

Veronika Nemcíková Villímová

The centrosome, the major microtubule organizing center in animal cells, is composed of two orthogonally arranged centrioles and surrounding pericentriolar material. Centrioles are evolutionary conserved organelles characterized by a nine-fold symmetry of ...

EPFL2018

Stabilization of overlapping biofilaments by passive crosslinkers

Ignacio Pagonabarraga Mora

The formation, maintenance and reorganization of the cytoskeletal filament network is essential for a number of cellular processes. While the crucial role played by active forces generated by motor proteins has been studied extensively, only recently the i ...

IOP Publishing - EPL Association2018

Multicolor single-particle reconstruction of protein complexes

Pierre Gönczy, Suliana Manley, Kyle Michael Douglass, Christian Sieben, Niccolo Banterle

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

2018

Advances in High-Speed Atomic Force Microscopy

Adrian Pascal Nievergelt

High-speed atomic force microscopy (HS-AFM) is a scanning probe technique capable of recording processes at the nanometre scale in real time. By sequentially increasing the speed of individual microscope components, images of surfaces can be recorded at up ...

EPFL2018

Probing the distribution of cortical components, including PtdIns(4,5)P2, during asymmetric division of C. elegans embryos

Melina Jasmin Scholze

Asymmetric cell division is crucial for embryonic development and stem cell lineages. In the one-cell C. elegans embryo, a contractile cortical actomyosin network contributes to asymmetric division by segregating PAR proteins to discrete cortical domains. ...

EPFL2018

High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

Pierre Gönczy, Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Niccolo Banterle

The self-assembly of protein complexes is at the core of many fundamental biological processes1, ranging from the polymerization of cytoskeletal elements, such as microtubules2, to viral capsid formation and organelle assembly3. To reach a comprehensive un ...

2018

Centriole Biogenesis: From Identifying the Characters to Understanding the Plot

Pierre Gönczy, Niccolo Banterle

The centriole is a beautiful microtubule-based organelle that is critical for the proper execution of many fundamental cellular processes, including polarity, motility, and division. Centriole biogenesis, the making of this miniature architectural wonder, ...

Annual Reviews2017