Publication

Consensus guidelines for the use and interpretation of angiogenesis assays

Publications associées (24)

Apelin-driven endothelial cell migration sustains intestinal progenitor cells and tumor growth

Mauro Delorenzi, Stefanie Siegert, Pascale Anderle Pedone, Nadine Fournier, Sina Nassiri, François Renevey, Michele De Palma, Mario Leonardo Squadrito, Simone Ragusa

Stem and progenitor cells residing in the intestinal crypts drive the majority of colorectal cancers (CRCs), yet vascular contribution to this niche remains largely unexplored. Vascular endothelial growth factor A (VEGFA) is a key driver of physiological a ...

Springernature2022

The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner

Michele De Palma

Neuro-vascular communication is essential to synchronize central nervous system development. Here, we identify angiopoietin/Tie2 as a neuro-vascular signaling axis involved in regulating dendritic morphogenesis of Purkinje cells (PCs). We show that in the ...

CELL PRESS2021

Ultraflexible flow directed device and system

Lucio Feo Pancaldi-Giubbini, Pietro Luciano Jakobus Dirix, Mahmut Selman Sakar

A flow directed device adapted to be guided by a fuid, such as blood, flow within a tubular structure such as a blood vessel is disclosed, said device comprising a proximal end (100), a distal end (101) and an elongated body (102) in between defining a lon ...

2021

Anti-angiogenic immunotherapy for lung cancer

Ece Kadioglu

Lung cancer is the leading cause of cancer-associated deaths worldwide. Platinum-based chemotherapy is the most common therapeutic approach in advanced non-small cell lung cancer (NSCLC), particularly for tumors that carry non-druggable activating mutation ...

EPFL2019

Characterization of a B16-F10 melanoma model locally implanted into the ear pinnae of C57BL/6 mice

Witold Waldemar Kilarski, Esra Güç

The common experimental use of B16-F10 melanoma cells focuses on exploring their metastatic potential following intravenous injection into mice. In this study, B16-F10 cells are used to develop a primary tumor model by implanting them directly into the ear ...

2018

Local induction of lymphangiogenesis with engineered fibrin-binding VEGF-C promotes wound healing by increasing immune cell trafficking and matrix remodeling

Jeffrey Alan Hubbell, Melody Swartz, Priscilla Suhasna Maithili Briquez, Witold Waldemar Kilarski, Esra Güç, Manuel Andreas Fankhauser

Lymphangiogenesis occurs in inflammation and wound healing, yet its functional roles in these processes are not fully understood. Consequently, clinically relevant strategies for therapeutic lymphangiogenesis remain underdeveloped, particularly using growt ...

2017

Design principles for therapeutic angiogenic materials

Mikaël Martino, Jeffrey Alan Hubbell, Priscilla Suhasna Maithili Briquez

Despite extensive research, pro-angiogenic drugs have failed to translate clinically, and therapeutic angiogenesis, which has potential in the treatment of various cardiovascular diseases, remains a major challenge. Physiologically, angiogenesis - the proc ...

Nature Publishing Group2016

FOXC2 and fluid shear stress stabilize postnatal lymphatic vasculature

Mauro Delorenzi, Yan Agalarov, Esther Bovay, Nadine Fournier

Biomechanical forces, such as fluid shear stress, govern multiple aspects of endothelial cell biology. In blood vessels, disturbed flow is associated with vascular diseases, such as atherosclerosis, and promotes endothelial cell proliferation and apoptosis ...

American Society for Clinical Investigation2015

Perivascular M2 macrophages stimulate tumor relapse after chemotherapy

Ioanna Keklikoglou, Michele De Palma

Tumor relapse after chemotherapy-induced regression is a major clinical problem, because it often involves inoperable metastatic disease. Tumor-associated macrophages (TAM) are known to limit the cytotoxic effects of chemotherapy in preclinical models of c ...

American Association for Cancer Research2015

Cancer metastasis: Perivascular macrophages under watch

Michele De Palma, Ece Kadioglu

TIE2-expressing macrophages cluster around blood vessels and sustain tumor angiogenesis. Harney and colleagues now use live imaging of mouse mammary tumors to show that these perivascular macrophages also promote the transient opening of tumor blood vessel ...

American Association for Cancer Research2015

Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation

Carl Petersen, Graham Knott, Natalya Korogod

Analysis of brain ultrastructure using electron microscopy typically relies on chemical fixation. However, this is known to cause significant tissue distortion including a reduction in the extracellular space. Cryo fixation is thought to give a truer repre ...

Elife Sciences Publications Ltd2015

Mathematical and Numerical Modeling of Healthy and Unhealthy Cerebral Arterial Tissues

Paolo Tricerri

Over the last two decades, we have witnessed an increasing application of mathematical models and numerical simulations for the study of the cardiovascular system. Indeed, both tools provide an important contribution to the analysis of the functioning of t ...

EPFL2014

The promotion of endothelial cell attachment and spreading using FNIII10 fused to VEGF-A(165)

Mikaël Martino, Jeffrey Alan Hubbell, Melody Swartz

Synergy in the downstream signaling pathways of the vascular endothelial growth factor receptor-2 (VEGFR-2) and the integrin alpha v beta 3 is critical for blood vessel formation. Thus, agents that activate both receptors could possess efficient pro-angiog ...

Elsevier2013

Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression

Douglas Hanahan

Endothelial cells (ECs) in blood vessels under formation are stabilized by the recruitment of pericytes, both in normal tissues and during angiogenesis in pathologic situations, including neoplasia. In the tumor vasculature, besides supporting the function ...

2011

Macrophage skewing by Phd2 haplodeficiency prevents ischaemia by inducing arteriogenesis

Michele De Palma, Mario Leonardo Squadrito, Francine Jordan

PHD2 serves as an oxygen sensor that rescues blood supply by regulating vessel formation and shape in case of oxygen shortage. However, it is unknown whether PHD2 can influence arteriogenesis. Here we studied the role of PHD2 in collateral artery growth by ...

2011

Identification and Functional Response of "Interstitial Cells of Cajal"-like Cells from Rat Mesenteric Artery

Aurélie Formey de Saint Louvent

This thesis is a contribution to the field of interstitial cells of Cajal-like cells (ICC-LCs) in the vasculature. Indeed, cells with irregular shapes and numerous long thin filaments, morphologically similar to the gastrointestinal ICCs, have been observe ...

EPFL2011

VE-statin/egfl7 expression in endothelial cells is regulated by a distal enhancer and a proximal promoter under the direct control of Erg and GATA-2

Friedrich Beermann

Angiogenesis is the process by which new blood vessels arise from existing ones by the budding out of endothelial cell capillaries from the luminal side of blood vessels. Blood vessel formation is essential for organ development during embryogenesis and is ...

Public Library of Science2010

Biomechanical regulation of blood vessel growth during tissue vascularization

Witold Waldemar Kilarski

Formation of new vessels in granulation tissue during wound healing has been assumed to occur solely through sprouting angiogenesis. In contrast, we show here that neovascularization can be accomplished by nonangiogenic expansion of preexisting vessels. Us ...

2009

Differential effects of reduced cyclic stretch and perturbed shear stress within the arterial wall

Tyler Nerton Thacher

Due to the pulsatile nature of blood flow, arteries are constantly exposed to dynamic mechanical forces; the pulsatility continuously stretches the vessel wall and the flow creates a frictional force on the interior surface. These stresses, referred to as ...

EPFL2009

c-myc in the hematopoietic lineage is crucial for its angiogenic function in the mouse embryo

The c-myc proto-oncogene, which is crucial for the progression of many human cancers, has been implicated in key cellular processes in diverse cell types, including endothelial cells that line the blood vessels and are critical for angiogenesis. The de nov ...

2008