Publications related to Glycogen phosphorylase

Investigation of physiological roles of AMPK-activated protein kinase y3

Clinical trials have shown that direct activators of an evolutionary-conserved metabolic sensor, AMP-activated protein kinase (AMPK), are beneficial in preventing/treating a range of metabolic disorders, including type 2 diabetes. These activators, includi ...

EPFL2021

Glycogen metabolism is impaired in the brain of male type 2 diabetic Goto‐Kakizaki rats

João Miguel das Neves Duarte, Ana Francisca Leal Silva Soares

Diabetes impacts the central nervous system predisposing to cognitive decline. While glucose is the main source of energy fueling the adult brain, brain glycogen is necessary for adequate neuronal function, synaptic plasticity and memory. In this study, we ...

2019

Ultra high magnetic field for glial contribution into brain metabolism studied by MR spectroscopy and CEST methods for molecular imaging of glycogen

Elise Marie Catherine Vinckenbosch

Magnetic resonance at ultra-high field increases signal and spectral dispersion. In this thesis, I use those characteristics to investigate three different subjects in 13C spectroscopy, hyperpolarized methods and chemical exchange saturation transfer (CEST ...

EPFL2018

Technical and experimental features of Magnetic Resonance Spectroscopy of brain glycogen metabolism

Rolf Gruetter, Hongxia Lei, Ana Francisca Leal Silva Soares

In the brain, glycogen is a source of glucose not only in emergency situations but also during normal brain activity. Altered brain glycogen metabolism is associated with energetic dysregulation in pathological conditions, such as diabetes or epilepsy. Bot ...

Elsevier2017

Glycogen Supercompensation in the Rat Brain After Acute Hypoglycemia is Independent of Glucose Levels During Recovery

Rolf Gruetter, João Miguel das Neves Duarte

Patients with diabetes display a progressive decay in the physiological counter-regulatory response to hypoglycemia, resulting in hypoglycemia unawareness. The mechanism through which the brain adapts to hypoglycemia may involve brain glycogen. We tested t ...

Springer Verlag2017

Functional analysis of Bicc1 and novel interacting proteins in renal tubule cell metabolism and polycystic kidneys

Lucía Carolina Leal Esteban

Genetic disorders known as ciliopathies develop polycystic kidneys, including autosomal dominant and autosomal recessive polycystic kidney diseases (ADPKD and ARPKD). Several signaling pathways including the cAMP/PKA pathway are implicated in driving cyst ...

EPFL2017

Three-dimensional immersive virtual reality for studying cellular compartments in 3D models from EM preparations of neural tissues

Pierre Magistretti, Corrado Cali, Daniya Boges, Madhusudhanan Srinivasan

Advances in the application of electron microscopy (EM) to serial imaging are opening doors to new ways of analyzing cellular structure. New and improved algorithms and workflows for manual and semiautomated segmentation allow us to observe the spatial arr ...

Wiley-Blackwell2016

AMP-activated protein kinase : strategies for activation and downstream target identification

Serge Thanh Son Ducommun

AMP-activated protein kinase (AMPK) is an evolutionally conserved key sensor of cellular energy status. Under conditions of cellular energy stress, AMPK is activated and will switch off anabolic processes that consume ATP, while activating catabolic proces ...

EPFL2016

Glutamate Cysteine Ligase-Modulatory Subunit Knockout Mouse Shows Normal Insulin Sensitivity but Reduced Liver Glycogen Storage

Pierre Magistretti, Kim Do

Glutathione (GSH) deficits have been observed in several mental or degenerative illness, and so has the metabolic syndrome. The impact of a decreased glucose metabolism on the GSH system is well-known, but the effect of decreased GSH levels on the energy m ...

Frontiers Media Sa2016

Regulation Of Neuron-Astrocyte Metabolic Coupling Across The Sleep-Wake Cycle

Pierre Magistretti, Jean-Marie Petit

Over the last thirty years, a growing number of studies showed that astrocytes play a pivotal role in the energy support to synapses. More precisely, astrocytes adjust energy production to neuronal energy needs through different mechanisms grouped under th ...

Elsevier2016

Imaging liver and brain glycogen metabolism at the nanometer scale

Anders Meibom, Graham Knott, Arnaud Comment, Stéphane Laurent Escrig, Yuhei Takado, Mojgan Masoodi

In mammals, glycogen synthesis and degradation are dynamic processes regulating blood and cerebral glucose-levels within a well-defined physiological range. Despite the essential role of glycogen in hepatic and cerebral metabolism, its spatiotemporal distr ...

Elsevier Science Bv2015

Glycogen metabolism and the homeostatic regulation of sleep

Pierre Magistretti, Igor Allaman, Jean-Marie Petit, Sophie Burlet Godinot

In 1995 Benington and Heller formulated an energy hypothesis of sleep centered on a key role of glycogen. It was postulated that a major function of sleep is to replenish glycogen stores in the brain that have been depleted during wakefulness which is asso ...

Springer Verlag2015

Disrupting astrocyte-neuron lactate transfer persistently reduces conditioned responses to cocaine

Pierre Magistretti, Jean-Luc Martin, Benjamin Boutrel, Benjamin Boury-Jamot, Anthony Carrard

A central problem in the treatment of drug addiction is the high risk of relapse often precipitated by drug-associated cues. The transfer of glycogen-derived lactate from astrocytes to neurons is required for long-term memory. Whereas blockade of drug memo ...

Nature Publishing Group2015

Deciphering the astrocyte-neuron metabolic cooperation in vivo

Manuel Raphael Zenger

Brain energy consumption is high and a very tightly regulated energy metabolism ensures correct performance. Neurons, the cells responsible for transmitting information via synapses, interact very closely with star shaped glial cells called astrocytes. Ast ...

EPFL2015

Subcellular Investigation of Photosynthesis-Driven Carbon and Nitrogen Assimilation and utilization in the Symbiotic Reef Coral: Pocillopora damicornis

Anders Meibom, Stéphane Laurent Escrig, Christophe Kopp, Isabelle Domart-Coulon

UNLABELLED: Reef-building corals form essential, mutualistic endosymbiotic associations with photosynthetic Symbiodinium dinoflagellates, providing their animal host partner with photosynthetically derived nutrients that allow the coral to thrive in oligot ...

American Society for Microbiology2015

A comparison of in vivo (13) C MR brain glycogen quantification at 9.4 and 14.1 T

Rolf Gruetter, Yves Pilloud, Ruud van Heeswijk

The high molecular weight and low concentration of brain glycogen render its noninvasive quantification challenging. Therefore, the precision increase of the quantification by localized (13) C MR at 9.4 to 14.1 T was investigated. Signal-to-noise ratio inc ...

Wiley2012

Altered Glycogen Metabolism in Cultured Astrocytes from Mice with Chronic Glutathione Deficit; Relevance for Neuroenergetics in Schizophrenia

Pierre Magistretti, Igor Allaman, Kim Do, Jean-Marie Petit

Neurodegenerative and psychiatric disorders including Alzheimer's, Parkinson's or Huntington's diseases and schizophrenia have been associated with a deficit in glutathione (GSH). In particular, a polymorphism in the gene of glutamate cysteine ligase modul ...

Public Library of Science2011

Astrocyte-neuron lactate transport is required for long-term memory formation

We report that, in the rat hippocampus, learning leads to a significant increase in extracellular lactate levels that derive from glycogen, an energy reserve selectively localized in astrocytes. Astrocytic glycogen breakdown and lactate release are essenti ...

2011

Quantification of brain glycogen concentration and turnover through localized C-13 NMR of both the C1 and C6 resonances

Rolf Gruetter, Lijing Xin, Ruud van Heeswijk

We have recently shown that at isotopic steady state C-13 NMR can provide a direct measurement of glycogen concentration changes, but that the turnover of glycogen was not accessible with this protocol. The aim of the present study was to design, implement ...

Wiley-Blackwell2010

Metabolic response of the cerebral cortex following gentle sleep deprivation and modafinil administration

Pierre Magistretti, Jean-Marie Petit, Christophe Kopp

Objectives: The main energy reserve of the brain is glycogen, which is almost exclusively localized in astrocytes. We previously reported that cerebral expression of certain genes related to glycogen metabolism changed following instrumental sleep deprivat ...

2010