Publications related to Minocycline

Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Camille Véronique Bernadette Goemans, Christian Eugen Zimmerli, Martin Beck

Ribosomes translate the genetic code into proteins. Recent technical advances have facilitated in situ structural analyses of ribosome functional states inside eukaryotic cells and the minimal bacterium Mycoplasma. However, such analyses of Gram-negative b ...

Nature Research2024

Long-term imaging, automated high-content phenotyping and sorting of C. elegans larvae and embryos using microfluidic platforms

Hüseyin Baris Atakan

C. elegans is frequently studied to address fundamental biological questions motivated by its short life cycle, small size, hermaphrodite behavior and well-annotated genome. In this work, we present novel microfluidic platforms that support long-term cultu ...

EPFL2019

Microfluidic high-content phenotypic screening on a Caenorhabditis elegans model

Maria Cristina Letizia

Caenorhabditis elegans is a performant model system for studying human biological processes and diseases and for pre-clinical phenotyping screenings of compounds. Microfluidics has been instrumental in enabling C. elegans-based drug assays, facilitating th ...

EPFL2018

A design of experiment approach for efficient multi-parametric drug testing using a Caenorhabditis elegans model

Martinus Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia, Giuseppe Tranchida

When studying the drug effectiveness towards a target model, one should distinguish the effects of the drug itself and of all the other factors that could influence the screening outcome. This comprehensive knowledge is crucial, especially when model organ ...

2018

Mitochondrial stress signalling and its impacts on physiology

Adrienne Joëlle Laurence Mottis

Mitochondria are responsible for respiration and energy harvesting across the eukaryotic kingdom. They also take part in other cellular processes like intermediary metabolism. As a result, mitochondrial function directly impacts organismal features such as ...

EPFL2018

Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research

Johan Auwerx, Bart Deplancke, Richardus Houtkooper, Laurent Mouchiroud, Dongryeol Ryu, Xu Wang, Evan Graehl Williams, Adrienne Joëlle Laurence Mottis, Virginija Jovaisaite, Norman Lionel Moullan, Michael Vincent Frochaux

In recent years, tetracyclines, such as doxycycline, have become broadly used to control gene expression by virtue of the Tet-on/Tet-off systems. However, the wide range of direct effects of tetracycline use has not been fully appreciated. We show here tha ...

Elsevier2015

Tetracycline Antibiotics Impair Mitochondrial Function and Its Experimental Use Confounds Research

Johan Auwerx, Laurent Mouchiroud, Richardus Houtkooper

Tetracyclines, a class of antibiotics that target bacterial translation, are commonly used in research for inducible gene expression using Tet-ON/Tet-OFF systems. However, such tetracycline-inducible systems carry a risk. Given that mitochondria have a "ba ...

American Association for Cancer Research2015

C-linked disaccharide analogue of the Thomsen-Friedenreich (T)-epitope alpha-O-conjugated to L-serine

Pierre Vogel, Loay Awad

Condensation of a silylated beta-D-galactopyranosylaldehyde (3) with isolevoglucosenone (4) in the presence of Et(2)AII provided bicyclic enone 5. Subsequent addition of BnNHOMe gave adduct 6, which was converted into 4-O-acetyl-1,6-anhydro-3-C-[ R)1.3,4,5 ...

2005

Long-term doxycycline-regulated secretion of erythropoietin by encapsulated myoblasts

Patrick Aebischer, Bernard Schneider, Nicole Déglon

We developed an ex vivo gene therapy approach for the regulated delivery of therapeutic proteins based on the implantation of encapsulated, genetically engineered C(2)C(12) myoblasts. We investigated doxycycline-based regulation of gene expression to modul ...

2002