Publications related to Membrane biology

Liposomes and Lipid Droplets Display a Reversal of Charge-Induced Hydration Asymmetry

Sylvie Roke, Nathan Dupertuis, Saranya Pullanchery Sankara Narayanan

The unique properties of water are critical for life. Water molecules have been reported to hydrate cations and anions asymmetrically in bulk water, being a key element in the balance of biochemical interactions. We show here that this behavior extends to ...

Amer Chemical Soc2023

Simultaneous Electrophysiology and Imaging Reveal Changes in Lipid Membrane Thickness and Tension upon Uptake of Amphiphilic Gold Nanoparticles

Francesco Stellacci, Xufeng Xu, Paulo Henrique Jacob Silva, Camilla Servidio

Amphiphilic gold core nanoparticles (AmNPs) striped with hydrophilic 11-mercapto-1-undecanesulfonate (MUS) and hydrophobic 1-octanethiol (OT) ligands are promising candidates for drug carriers that passively and nondisruptively enter cells. Yet, how they i ...

Amer Chemical Soc2023

Infrared nanoplasmonic metasurfaces augmented by artificial intelligence for universal biosensing

Aurélian Michel John-Herpin

Nanoplasmonic metasurfaces have shown outstanding light-matter interaction enhancement capabilities, leading to their emergence as powerful platforms for highly sensitive biospectroscopy. Metasurface-enhanced biospectroscopy offers unprecedented opportunit ...

EPFL2022

2D MoS2 Nanopores: Wafer-scale Fabrication and Monolayer Stability for Long-term Single-Molecule Sensing

Mukeshchand Thakur

Biologically inspired solid-state nanopores are artificial openings or apertures in thin membranes similar to natural protein ion channels in a lipid bilayer of cell membranes. In solid-state nanopores, a thin insulating membrane with single or multiple po ...

EPFL2022

Imaging Cu2+ binding to charged phospholipid membranes by high-throughput second harmonic wide-field microscopy

Sylvie Roke, Seonwoo Lee, David Roesel

The interaction of divalent copper ions (Cu2+) with cell membranes is crucial for a variety of physiological processes of cells, such as hormone synthesis and cellular energy production. These interactions would not be possible without membrane hydration. ...

AIP Publishing2021

Transient domains of ordered water induced by divalent ions lead to lipid membrane curvature fluctuations

Sylvie Roke, Orly Bagunu Tarun, Halil Ibrahim Okur

Cations influence the electrostatic, chemical and mechanical properties of lipid bilayers. Here, label-free second harmonic microscopy shows that cation-induced transient ordering of water also plays a role in driving membrane curvature fluctuations, linki ...

NATURE PUBLISHING GROUP2020

Soft matter probed by nonlinear scattering: self-assembly, interfaces, hydration, and long-range order

Jan Dedic

All organisms are made to a large extent of soft matter - macromolecules such as proteins and polysaccharides, or assemblies of small molecules such as lipids embedded in an aqueous environment. Understanding the role of order in soft matter presents a cha ...

EPFL2020

High-permeance polymer-functionalized single-layer graphene membranes that surpass the postcombustion carbon capture target

Emad Oveisi, Kumar Varoon Agrawal, Mounir Driss Mensi, Luis Francisco Villalobos Vazquez de la Parra, Shiqi Huang, Guangwei He, Jing Zhao

Membrane-based postcombustion carbon capture can reduce the capture penalty in comparison to absorbent-based separation. To realize this, high-performance membranes are urgently needed with a CO2 permeance exceeding 1000 gas permeation units or GPU, and a ...

2019

Microfluidic fabrication of vesicles with hybrid lipid/nanoparticle bilayer membranes

Esther Amstad

Hybrid lipid/nanoparticle membranes are suitable model systems both to study the complex interactions between nanoparticles and biological membranes, and to demonstrate technological concepts in cellular sensing and drug delivery. Unfortunately, embedding ...

ROYAL SOC CHEMISTRY2019

Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales

Sylvie Roke, Orly Bagunu Tarun

Biological membranes are highly dynamic and complex lipid bilayers, responsible for the fate of living cells. To achieve this function, the hydrating environment is crucial. However, membrane imaging typically neglects water, focusing on the insertion of p ...

2018

Pre-transition effects mediate forces of assembly between transmembrane proteins

Berend Smit

We present a mechanism for a generic, powerful force of assembly and mobility for transmembrane proteins in lipid bilayers. This force is a pre-transition (or pre-melting) effect for the first-order transition between ordered and disordered phases in the m ...

Elife Sciences Publications Ltd2016

Lipid Builder: A Framework To Build Realistic Models for Biological Membranes

Matteo Dal Peraro, Thomas Lemmin, Giorgio Elikem Tamo, Christophe Bovigny, Nicolas Grégoire Maïno

The physical and chemical characterization of biological membranes is of fundamental importance for understanding the functional role of lipid bilayers in shaping cells and organelles, steering vesicle trafficking and promoting membrane-protein signaling. ...

Amer Chemical Soc2015

In situ synthesis and simulation of polydisperse amphiphilic membranes

Lida Kanari, Julian Charles Shillcock, Liesbeth Maria L Vanherpe, Guy Antoine Atenekeng Kahou, Juan Pablo Palacios

The field of coarse-grained simulations of biopolymers and membranes has grown rapidly in recent years. Industrial groups manufacture and use polymers in fields as diverse as chemicals processing and personal care products, while academic researchers are i ...

Springer India2015

The dynamics of a capsule in a wall-bounded oscillating shear flow

Lailai Zhu

The motion of an initially spherical capsule in a wall-bounded oscillating shear flow is investigated via an accelerated boundary integral implementation. The neo-Hookean model is used as the constitutive law of the capsule membrane. The maximum wall-norma ...

Amer Inst Physics2015

Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes

Daniel Sage

Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact prob ...

Mdpi Ag2014

Perturbations of the Straight Transmembrane alpha-Helical Structure of the Amyloid Precursor Protein Affect Its Processing by gamma-Secretase

Matteo Dal Peraro, Patrick Fraering, Mitko Dimitrov, Thomas Lemmin

Background: Amyloid- neurotoxicity depends on the specificity of the proteolytic cleavage of the amyloid precursor protein (APP) transmembrane domain. Results: The APP transmembrane -helix is straight in a biological membrane bilayer. Conclusion: The flexi ...

American Society for Biochemistry and Molecular Biology2014

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red

Kai Johnsson, Rudolf Hovius, Luc Reymond, Howard Riezman, Efthymia Prifti

A fluorogenic probe for plasma membrane proteins based on the dye Nile Red and SNAP-tag is introduced. It takes advantage of Nile Red, a solvatochromic molecule highly fluorescent in an apolar environment, such as cellular membranes, but almost dark in a p ...

Amer Chemical Soc2014

Molecular View of Cholesterol Flip-Flop and Chemical Potential in Different Membrane Environments

Marlon Hinner

The relative stability of cholesterol in cellular membranes and the thermodynamics of fluctuations from equilibrium have important consequences for sterol trafficking and lateral domain formation. We used molecular dynamics computer simulations to investig ...

2009

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Horst Vogel, Jean-Manuel Segura, Jean-Baptiste Perez

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

2006

Two-phase partition profiling of drugs and ionisable compounds

Lipophilicity of compounds with therapeutic interest represents one of the most important physicochemical parameters in predicting and interpreting transport processes across biological barriers. This property is commonly measured as a drug | membrane part ...

EPFL2004