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Related publications (28)

MAR Elements and Transposons for Improved Transgene Integration and Expression

Nicolas Mermod, Déborah Ley, Niamh Harraghy

Reliable and long-term expression of transgenes remain significant challenges for gene therapy and biotechnology applications, especially when antibiotic selection procedures are not applicable. In this context, transposons represent attractive gene transf ...

2013

Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak

For most cultivated mammalian cells, glutamine is an essential medium component. However, glutamine consumption results in the production of ammonia, a cytotoxic byproduct. Here we investigated the effect of glutamine reduction on recombinant protein produ ...

Springer Netherlands2011

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sagar Shashidhar Manoli

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Harm-Anton Klok, Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Zuzana Kadlecova, Yashas Rajendra

The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in I ...

2012

Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture

Francesca Zagari

Mammalian cells represent the most widely used host system for the industrial production of recombinant therapeutic proteins. In order to increase productivity, chemically defined media are often used and optimized to provide the cells with the necessary n ...

EPFL2012

Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors

Florian Maria Wurm

Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today's technical status and to highlight emerging trends in the manufacture of recombinant therapeutic proteins, focusing on Chinese hamster ovary ...

Elsevier2011

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

Florian Maria Wurm, David Hacker, Lucia Baldi Unser

Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable ...

Wiley-Blackwell2011

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Florian Maria Wurm, Mohamed Farhat, David Hacker, Lucia Baldi Unser, Matthieu Dreyer, Stéphanie Tissot, Martino Reclari, Agata Oberbek

Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are ...

Elsevier2011

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