Publications related to Dark-field microscopy

Advances in High-Speed, Multiparametric Atomic Force Microscopy

After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...

EPFL2024

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

François Thierry M Marelli

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

EPFL2023

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Method and light microscope with a plurality of arrays of photon-counting detector elements

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic

A method for operating a light microscope comprises emitting and guiding a plurality of illumination light beams towards a specimen (6) to form a plurality of separated illumination light spots (2A, 2B, 2C, 2D) at the specimen; and guiding detection light ...

2022

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Arne Seitz, Andrea Bassi, Antje Keppler

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are fou ...

WILEY2021

Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy

Kai Johnsson, Lu Wang

Rhodamines are the most important class of fluorophores for applications in live-cell fluorescence microscopy. This is mainly because rhodamines exist in a dynamic equilibrium between a fluorescent zwitterion and a nonfluorescent but cell-permeable spirocy ...

AMER CHEMICAL SOC2021

Organisation and dynamics of mitochondria and their RNA

Timo Henry René Rey

The organisation of molecules into dynamic cells, and collaboration of many of those cells over a billion years led to the evolution of human life. During the last century, biologists then began to unravel the marvels of cellular organisation with ever inc ...

EPFL2021

Active illumination and computational Methods for Temporal and Spectral Super-Resolution Microscopy

Christian Jaques

Light microscopy is a tool of paramount importance for biologists and has been constantly improved for the past four centuries. Despite many recent developments, microscopy techniques still require improvement, especially to reach better temporal and spect ...

EPFL2020

Time-lapse high-resolution microscopy to study the morphogenesis of microorganisms

Mélanie Thérèse Marie Hannebelle

Mycobacterium tuberculosis, the etiological agent for the tuberculosis disease, is a bacterial pathogen thought to infect about a quarter of the global human population. It is the first cause of death among infectious diseases, and is most prevalent in low ...

EPFL2020

Do Lewy bodies contain alpha-synuclein fibrils? and Does it matter? A brief history and critical analysis of recent reports

Hilal Lashuel

Several lines of evidence from neuropathological studies, human genetics, in vitro aggregation studies and cellular and animal models support the hypothesis that aSyn plays a central role in the formation of Lewy pathologies. These are cytoplasmic proteina ...

2020

Transmission Microsphere-Assisted Dark-Field Microscopy

Giorgio Quaranta, Stéphane Perrin, Hongyu Li

Microsphere-assisted microscopy allows the limit of the diffraction of light to overcome while being non-invasive, full-field, label-free, and easy-to-implement. However, the observation of translucent samples remains difficult using a classical bright-fie ...

WILEY-V C H VERLAG GMBH2019

Virtual High-Framerate Microscopy of the Beating Heart via Sorting of Still Images

Michael Stefan Daniel Liebling, Olivia Mariani

Microscopy of the beating heart in embryos provides key insights for the study of its development. However, achieving a sufficiently high framerate is difficult with conventional cameras. Here, we present a method to reconstruct an image sequence covering ...

Idiap2019

High-resolution interference microscopy with spectral resolution for the characterization of individual particles and self-assembled meta-atoms

Toralf Scharf, Michail Symeonidis, Andreas Vetter

We apply a high-resolution interference microscope with spectral resolution to investigate the scattering response of isolated meta-atoms in real space. The final meta-atoms consist of core-shell clusters that are fabricated using a bottom-up approach. The ...

2019

Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroi ...

2019

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Near-field investigation of Bloch surface wave based 2D optical components

Hans Peter Herzig, Myun Sik Kim, Elsie Barakat, Richa Dubey, Babak Vosoughi Lahijani

We study the Bloch surface wave based nano-thin 2D optical components. The 2D elements are fabricated on the dielectric multilayer platform which sustains the Bloch surface waves. Such a platform is considered as a novel foundation for planar integrated op ...

Spie-Int Soc Optical Engineering2017

Simultaneous temporal superresolution and denoising for cardiac fluorescence microscopy

Michael Stefan Daniel Liebling

Due to low light emission of fluorescent samples, live fluorescence microscopy imposes a tradeoff between spatiotemporal resolution and signal-to-noise ratio. This can result in images and videos containing motion blur or Poisson-type shot noise, depending ...

2016

Ultrafast imaging of plasmons in a transmission electron microscope

Fabrizio Carbone, Gabriele Berruto, Tom Theodorus Antonius Lummen

Miniaturized plasmonic and photonic integrated circuits are generally considered as the core of future generations of optoelectronic devices, due to their potential to bridge the size-compatibility gap between photonics and electronics. However, as the nan ...

Spie-Int Soc Optical Engineering2016

Sparsity-Based Data Reconstruction Models for Biomedical Imaging

Emrah Bostan

We propose new regularization models to solve inverse problems encountered in biomedical imaging applications. In formulating mathematical schemes, we base our approach on the sparse signal processing principles that have emerged as a central paradigm in t ...

EPFL2016

Mechanical and Functional Study of Cell Physiology at the Nanoscale

Pascal Damian Odermatt

By live-cell imaging of biological samples dynamic cellular processes can be resolved. Fluorescence microscopy (FM) and atomic force microscopy (AFM) are both capable of imaging live cells. By combining these techniques structural as well as functional inf ...

EPFL2016