Publications related to Bioreactor

Non-targeted facilitation of primary sludge anaerobic fermentation by micro-aeration and the simultaneous nutrients transformations

Mohammed Mouhib, Chenxi Liu, Lin Li, Qiang He

Anaerobic fermentation is a widely used technology for resource recovery from municipal sludge (e.g., primary sludge). Micro-aeration is an emerging strategy which can be incorporated into this technology. However, the effect of micro-aeration on the anaer ...

Elsevier Science Sa2024

Co-culture of microalgae-activated sludge for wastewater treatment and biomass production: Exploring their role under different inoculation ratios

Florian Frédéric Vincent Breider

In this study, mixed culture (microalgae:activated sludge) of a photobioreactor (PBR) were investigated at different inoculation ratios (1:0, 9:1, 3:1, 1:1, 0:1 wt/wt). This work was not only to determine the optimal ratio for pollutant remediation and bio ...

Elsevier2020

Labor division in engineered cross-kingdom consortia: Consolidated bioprocessing of lignocellulosic biomass to carboxylic acids

Robert Lawrence Shahab

Lignocellulose is a renewable source of fixed carbon and a promising substrate for the production of chemicals and fuels in second generation biorefineries. In this thesis, we engineered artificial microbial consortia for consolidated bioprocessing (CBP) o ...

EPFL2019

Modifier adaptation with guaranteed feasibility in the presence of gradient uncertainty

Dominique Bonvin, Timm Faulwasser, Alejandro Gabriel Marchetti, Martand Singhal

In the context of real-time optimization, modifier-adaptation schemes use estimates of the plant gradients to achieve plant optimality despite plant-model mismatch. Plant feasibility is guaranteed upon convergence, but not at the successive operating point ...

Pergamon-Elsevier Science Ltd2017

Enhanced simultaneous saccharification and fermentation of pretreated beech wood by in situ treatment with the white rot fungus Irpex lacteus in a membrane aerated biofilm reactor

Robert Lawrence Shahab

The aim of the present study was to investigate the combination of steam pretreatment and biological treatment with lignin degrading fungal strains in order to enable efficient bioprocessing of beech wood to ethanol. In a sequential process of steam and fu ...

Elsevier2017

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Marc Moniatte, Dominique Terry Monteil Jr, Valentin Juvet

Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...

American Chemical Society2016

Influence of the Sparger Type and Added Alcohol on the Gas Holdup of an External-Loop Airlift Reactor

The influences of alcohol adding and three different gas spargers (single orifice, perforated plate, and sinter plate) on the gas holdup in an external-loop airlift reactor were studied. The experimental results show that the gas holdup can be increased by ...

Wiley-V C H Verlag Gmbh2015

Model-Based Derivation, Analysis and Control of Unstable Microaerobic Steady-States-Considering Rhodospirillum rubrum as an Example

Timm Faulwasser

Microaerobic (oxygen-limited) conditions are critical for inducing many important microbial processes in industrial or environmental applications. At very low oxygen concentrations, however, the process performance often suffers from technical limitations. ...

Wiley-Blackwell2014

The Engineering of a Scalable Disposable Orbitally Shaken Bioreactor System for Animal Cell Cultivation and Therapeutic Protein Production

Dominique Terry Monteil Jr

The aim of the pharmaceutical industry is to manufacture complex therapeutic proteins or biologics to treat human diseases. The manufacture of biologics requires a significant amount of financial and infrastructure resources. For this reason, efforts are b ...

EPFL2014

Constant-yield control of continuous bioreactors

Georgios Savoglidis

The process of anaerobic digestion is a common application in the treatment of industrial and communal wastewater treatment plants. The biochemical reactions taking place inside the fermenter can be described by a system of first order differential equatio ...

Elsevier Science Sa2013

Numerical simulation of orbitally shaken viscous fluids with free surface

Alfio Quarteroni, Florian Maria Wurm, David Hacker, Marco Discacciati, Stéphanie Tissot, Samuel Quinodoz

Orbitally shaken bioreactors are an emerging alternative to stirred-tank bioreactors for large-scale mammalian cell culture, but their fluid dynamics is still not well defined. Among the theoretical and practical issues that remain to be resolved, the char ...

Wiley-Blackwell2013

OrbShake Bioreactors for Mammalian Cell Cultures : Engineering and Scale-up

Stéphanie Tissot

Most of the biopharmaceuticals nowadays are produced by animal cells. The worldwide demand for a specific group of biopharmaceuticals, the monoclonal antibodies (mAbs), has significantly increased this last decade. The mAbs are used as therapeutics in part ...

EPFL2011

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Mathieu Benjamin Stettler, Stéphanie Tissot, Xiaowei Zhang, Gilles Broccard

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Schweizerische Chemische Gesellschaft2010

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Sarah Wulhfard

Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yiel ...

Elsevier2010

Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors

Christos Comninellis, Florian Maria Wurm, Mohamed Farhat, David Hacker, Stéphanie Tissot

Efficient mixing in bioreactors is essential in order to avoid concentration gradients which can be harmful for mammalian cells. To study mixing and its scalability in orbitally shaken cylindrical bioreactors, we measured mixing times in containers with no ...

Elsevier2010

Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale

Florian Maria Wurm, Alfio Quarteroni, David Hacker, Mathieu Benjamin Stettler, Xi Zhang

Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel mammalian cell culture bioreactor systems for the rapid production of biologicals that require mammalian protein processing. Recently, orbitally sha ...

2009

Data Reconciliation of Concentration Estimates from Mid-Infrared and Dielectric Spectral Measurements for Improved On-Line Monitoring of Bioprocesses

Dominique Bonvin, Urs von Stockar, Ian William Marison, Michael Amrhein, Michal Dabros

Real-time data reconciliation of concentration estimates of process analytes and biomass in microbial fermentations is investigated. A Fourier-transform mid-infrared spectrometer predicting the concentrations of process metabolites is used in parallel with ...

2009

Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells

Mathieu Benjamin Stettler

The objective of industrial cell culture technology is to produce high value-added therapeutic proteins that are well suited for the treatment of infectious diseases, cancer, and autoimmune diseases. Nowadays, an important focus is the reduction of the tim ...

EPFL2007

Quantitative characterization of a recombinant Pichia pastoris Mut+ strain secreting avidin using transient continuous cultures

Carmen Jungo

The objective of this thesis was to characterize the growth stoichiometry, the specific recombinant protein productivity and the regulation of the alcohol oxidase (AOX) enzyme of a Pichia pastoris Mut+ strain, performing conventional chemostat cultures and ...

EPFL2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, David Hacker, Martin Jordan, Madiha Derouazi, Natalie Muller, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007