Publications by Etienne Cuche | EPFL Graph Search

Full field vertical scanning in short coherence digital holographic microscope

Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Christophe Moser

In Digital holography Microscopes (DHM) implemented in the so-called "off axis" configuration, the object and reference wave fronts are not co-planar but form an angle of a few degrees. This results into two main drawbacks. First, the contrast of the inter ...

Optical Soc Amer2013

Extended depth-of-focus by digital holographic microscopy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Axial sub-nanometer accuracy in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

2008

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

WAVE FRONT SENSING METHOD AND APPARATUS

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Florian Charrière, Mihaela Marian

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or ... A n ...

2006

Cell refractive index tomography by digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired ...

2006

Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière

We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such ...

2006

Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière, Mihaela Marian

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, m ...

2006

Characterization of microlenses by digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing. Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers. Measurements performed ...

2006

Shot noise influence in reconstructed phase image SNR in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

In digital holographic microscopy, shot noise is an intrinsic part of the recording process with the digital camera. We present a study based on simulations and real measurements describing the shot-noise influence in the quality of the reconstructed phase ...

2006

Purely numerical compensation for microscope objective phase curvature in digital holographic microscopy: influence of digital phase mask position

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

Introducing a microscope objective in an interferometric setup induces a phase curvature on the resulting wavefront. In digital holography, the compensation of this curvature is often done by introducing an identical curvature in the reference arm and the ...

2006

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Sylvain Herminjard

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off- axis geometry, of the interfe ...

2006

Time-domain optical coherence tomography with digital holographic microscopy

Pia Massatsch, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

We show that digital holography can be combined easily with optical coherence tomography approach. Varying the reference path length is the means used to acquire a series of holograms at different depths, providing after reconstruction images of slices at ...

2005

Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. ...

2005

Shot noise influence in reconstructed phase images SNR in digital holographic microscopy

Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

We present a study based on simulations describing how shot noise, an intrinsic part of the recording process with a digital camera, influences the quality of the reconstructed phase images under different beams intensities configurations. ...

SPIE2005

Digital holographic microscopy for study cellular dynamics

Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DH ...

SPIE2005

Polarization microscopy by use of digital holography: application to optical- fiber birefringence measurements

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Hans Georg Limberger, René Salathé, Pierre Marquet, Florian Dürr

We present a digital holographic microscope that permits one to image polarization state. This technique results from the coupling of digital holographic microscopy and polarization digital holography. The interference between two orthogonally polarized re ...

2005

Digital Holographic Microscopy, a new imaging technology applied to nanotechnology and life

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière, Mihaela Marian, Pierre Magistretti

2005

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsew ...

2005

Numerical reconstruction of digital holograms : application to phase-contrast imaging and microscopy

Etienne Cuche

This thesis presents a new method for the numerical reconstruction of digital holograms recorded in the off-axis configuration using a CCD camera. The propagation of the reconstructed wave front, from the hologram plane to an observation plane, is achieved ...

EPFL2000