Publications by Christian Depeursinge

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Christian Depeursinge, Pierre Marquet, Shanshan Kou, Chen Liu

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object ...

2019

Quantitative phase imaging in biomedicine

Christian Depeursinge

Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobl ...

Nature Publishing Group2018

Quantitative phase-digital holographic microscopy: a new imaging modality to identify original cellular biomarkers of diseases

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Benjamin Rappaz, Kaspar Rothenfusser

Quantitative phase microscopy (QPM) has recently emerged as a powerful label-free technique in the field of living cell imaging allowing to non-invasively measure with a nanometric axial sensitivity cell structure and dynamics. Since the phase retardation ...

Spie-Int Soc Optical Engineering2016

Towards an incoherent off-axis digital holographic microscope

Frédéric Montfort, Christian Depeursinge

We propose and experimentally demonstrate a system in which off-axis digital holographic microscopy is realized using a broadband illumination source. Single-shot holographic measurements are enabled, while the coherence noise is removed thanks to the broa ...

Spie-Int Soc Optical Engineering2015

Off-axis digital holographic camera for quantitative phase microscopy

Frédéric Montfort, Christian Depeursinge, Christophe Moser, Zahra Monemhaghdoust

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a sing ...

Optical Soc Amer2014

Portable advanced off-axis camera for quantitative phase microscopy

Christian Depeursinge

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission micro ...

Spie-Int Soc Optical Engineering2014

Full field vertical scanning in short coherence digital holographic microscope

Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Christophe Moser

In Digital holography Microscopes (DHM) implemented in the so-called "off axis" configuration, the object and reference wave fronts are not co-planar but form an angle of a few degrees. This results into two main drawbacks. First, the contrast of the inter ...

Optical Soc Amer2013

Quantitative phase noise in a two-color low coherence Digital Holographic Microscope

Frédéric Montfort, Christian Depeursinge, Christophe Moser, Zahra Monemhaghdoust

In digital holographic microscopy (DHM), the long coherence length of laser light causes parasitic interferences due to multiple reflections in and by optical components in the optical path of the microscope and thus degrades the image quality. The parasit ...

Spie-Int Soc Optical Engineering2013

A Practical Inverse-Problem Approach to Digital Holographic Reconstruction

Christian Depeursinge, Michaël Unser, Nicolas Pavillon, Aurélien Bourquard, Emrah Bostan

In this paper, we propose a new technique for high-quality reconstruction from single digital holographic acquisitions. The unknown complex object field is found as the solution of a nonlinear inverse problem that consists in the minimization of an energy ...

OSA2013

Apparatus and method for quantitative phase tomography through linear scanning with coherent and non-coherent detection

Christian Depeursinge, Nicolas Pavillon

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample ...

2013

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti

In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale p ...

Annual Reviews2013

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Gerardo Turcatti, Christian Depeursinge, Marc Chambon, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Julien Mena, Jérôme Parent, Etienne Shaffer

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Marker-free phase nanoscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Optical reconstruction of transparent objects with phase-only SLMs

Christian Depeursinge, Yves Delacrétaz, Cristian Arfire

Three approaches for visualization of transparent micro-objects from holographic data using phase-only SLMs are described. The objects are silicon micro-lenses captured in the near infrared by means of digital holographic microscopy and a simulated weakly ...

Optical Soc Amer2013

Reconstruction in interferometric synthetic aperture microscopy: comparison with optical coherence tomography and digital holographic microscopy

Christian Depeursinge, Shanshan Kou

It is shown that the spatial frequencies recorded in interferometric synthetic aperture microscopy do not correspond to exact backscattering [as they do in unistatic synthetic aperture radar (SAR)] and that the reconstruction process based on SAR is theref ...

2012

Single-shot, simultaneous incoherent and holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer

In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial fre ...

Wiley-Blackwell2012

Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor

Christian Depeursinge, Pierre Marquet, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Chloride channels represent a group of targets for major clinical indications. However, molecular screening for chloride channel modulators has proven to be difficult and time-consuming as approaches essentially rely on the use of fluorescent dyes or invas ...

Public Library of Science2012

Enhanced robustness digital holographic microscopy for demanding environment of space biology

Christian Depeursinge, Jonas Kühn, Muhammed Fatih Toy, Marcel Egli, Stéphane Richard

We describe an optimized digital holographic microscopy system (DHM) suitable for high-resolution visualization of living cells under conditions of altered macroscopic mechanical forces such as those that arise from changes in gravitational force. Experime ...

2012

Early Cell Death Detection with Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012