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Pierre Marquet

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Related publications (65)

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti

In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale p ...

Annual Reviews2013

Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells

Pierre Marquet, Daniel Boss

In this paper, we present an automated approach to quantify information about three-dimensional (3D) morphology, hemoglobin content and density of mature red blood cells (RBCs) using off-axis digital holographic microscopy (DHM) and statistical algorithms. ...

Optical Society of America2012

Spatially-Resolved Eigenmode Decomposition of Red Blood Cells Membrane Fluctuations Questions the Role of ATP in Flickering

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Dimitri Nestor Alice Van De Ville, Annick Hoffmann, Daniel Boss, Benjamin Rappaz

Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of ...

Public Library Science2012

Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor

Christian Depeursinge, Pierre Marquet, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Chloride channels represent a group of targets for major clinical indications. However, molecular screening for chloride channel modulators has proven to be difficult and time-consuming as approaches essentially rely on the use of fluorescent dyes or invas ...

Public Library of Science2012

Early Cell Death Detection with Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

What role in cerebral activity?

Pierre Marquet, Pierre Magistretti, Pascal Jourdain

2012

High b-value diffusion-weighted imaging: a sensitive method to reveal white matter differences in schizophrenia

Pierre Marquet, Jean-Philippe Thiran, Djalel Eddine Meskaldji, Patric Hagmann, Reto Meuli, Philipp Baumann, Leila Cammoun

Over the last ten years, Diffusion-weighted Imaging (DWI) has become an important tool to investigate white matter (WM) anomalies in schizophrenia. Despite technological improvement and the exponential use of this technique, discrepancies remain and little ...

Elsevier2012

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

Society for Neuroscience2011

Recognition and classification of red blood cells using digital holographic microscopy and data clustering with discriminant analysis

Pierre Marquet, Daniel Boss

We propose to apply statistical clustering algorithms on a three-dimensional profile of red blood cells (RBCs) obtained through digital holographic microscopy (DHM). We show that two classes of RBCs stored for 14 and 38 days can be effectively classified. ...

2011

Quantitative phase restoration by direct inversion using the optical transfer function

Christian Depeursinge, Pierre Marquet, Laura Waller, Shanshan Kou

Quantitative phase recovery of phase objects is achieved by a direct inversion using the defocused weak object transfer function. The presented method is noniterative and is based on partially coherent principles. It also takes into account the optical pro ...

Optical Society of America2011

Signification of the phase function parameter gamma explored with a fractal distribution of scatterers

Stéphane Chamot, Christian Depeursinge, Pierre Marquet, Elena Migacheva

The optical properties within limited volumes of diffusive media can be probed by carrying spatially-resolved measurements of diffused light at short source-detector separation (typically one scattering mean free path). At such distance, analytical models ...

Optical Society of America2010

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...

Optical Society of America2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Corinne Moratal, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Label-free second harmonic phase imaging of biological specimen by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Corinne Moratal, Etienne Shaffer

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of ...

Optical Society of America2010

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Annick Hoffmann, Daniel Boss, Benjamin Rappaz

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, posses ...

Elsevier2009

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Viesturs Simanis, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Nicolas Pavillon, Benjamin Rappaz, Corinne Moratal

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Optical recording of cell electrical activity by Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Red blood cell structure and dynamics explored with digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedance volume analyzer

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Benjamin Rappaz

Red blood cell (RBC) parameters such as morphology, volume, refractive index, and hemoglobin content are of great importance for diagnostic purposes. Existing approaches require complicated calibration procedures and robust cell perturbation. As a result, ...

2008

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