Publications by Martin Jordan | EPFL Graph Search

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into ...

Elsevier2013

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Pierre-André Besse, Radivoje Popovic, Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Franck Vincent, Klaus Ehrmann

NMR-microprobes based on solenoids and Helmholtz coils have been microfabricated and NMR-spectra of mammalian cells have successfully been taken. The microfabrication technology developed for these probes consists of three electroplated copper levels for l ...

2007

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Natalie Muller, David Hacker, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Madiha Derouazi

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, Martin Jordan, Martin Bertschinger, David Hacker, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, David Hacker, Nicolas Jaccard

We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugatio ...

2006

Polyethylenimine-based quality control assay for plasmid DNA

Florian Maria Wurm, Martin Jordan, Martin Bertschinger, David Hacker, Gaurav Backliwal

2006

Temperature measurements in microfluidic systems: Heat dissipation of negative dielectrophoresis barriers

Philippe Renaud, Martin Jordan, Silvan Schnydrig, Urban Seger

The manipulation of living biological cells in microfluidic channels by a combination of negative dielectrophoretic barriers and pressure-driven flows is widely employed in lab-on-a-chip systems. However, electric fields in conducting media induce Joule he ...

2005

Orbital shaker technology for the cultivation of mammalian cells in suspension

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Natalie Muller, David Hacker

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Low-temperature pausing of cultivated mammalian cells

Lisa Hunt, Florian Maria Wurm, Martin Jordan, Frédéric Grosjean, David Hacker

There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a v ...

2005

Mammalian cell culture productivity at large scale – from grams/liter to tens of grams/liter soon?

Florian Maria Wurm, Martin Jordan, David Hacker

2005

Pausing of mammalian cells by cold exposure, limits and opportunities

Lisa Hunt, Florian Maria Wurm, Martin Jordan, David Hacker

2003

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Philippe Pierre Girard, Florian Maria Wurm, Martin Jordan, Jason Wright, Lucia Baldi Unser, Frédéric Grosjean, Natalie Muller, David Hacker

Springer2002

Transient expression of a soluble and secreted form of heterodimeric T-Cell receptor in Hek-293

Florian Maria Wurm, Martin Jordan

Kluwer Academic1998