Publications by Lucia Baldi Unser

NanoElectronics roadmap for Europe: From nanodevices and innovative materials to system integration

Lucia Baldi Unser, Mihai Adrian Ionescu, Montserrat Fernandez-Bolanos Badia

The NEREID project ("NanoElectronics Roadmap for Europe: Identification and Dissemination") is dedicated to mapping the future of European Nanoelectronics. NEREID's objective is to develop a medium and long term roadmap for the European nanoelectronics ind ...

PERGAMON-ELSEVIER SCIENCE LTD2019

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

2018

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Giulia Tontodonati

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Super ...

Elsevier2016

User-Friendly Image-Based Segmentation and Analysis of Chromosomes

Florian Maria Wurm, Michaël Unser, Lucia Baldi Unser, Virginie Sophie Uhlmann, Ricard Delgado Gonzalo

We designed two efficient and user-friendly tools for the segmentation and analysis of images containing chromosomes or, more generally, rod-shaped elements that are spread on microscopic slides. The segmentation tool allows to automatically extract the pr ...

IEEE2016

Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Yashas Rajendra, Sowmya Balasubramanian

Several naturally occurring vertebrate transposable elements have been genetically modified to enable the transposition of recombinant genes in mammalian cells. We compared three transposonspiggyBac, Tol2, and Sleeping Beautyfor their ability to generate c ...

Wiley-Blackwell2016

A comparison of orbitally-shaken and stirred-tank bioreactors: pH modulation and bioreactor type affect CHO cell growth and protein glycosylation

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Marc Moniatte, Valentin Juvet, Dominique Terry Monteil Jr

Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank bioreactors (STRs) due to their rapid and homogeneous mixing and high oxygen transfer ra ...

American Chemical Society2016

Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Zuzana Kadlecova, Sowmya Balasubramanian

Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...

Elsevier2015

A simple plasmid-based transient gene expression method using High Five cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann, Xiao Shen

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Elsevier2015

Enhanced Plasmid DNA Utilization in Transiently Transfected CHO-DG44 Cells in the Presence of Polar Solvents

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sowmya Balasubramanian

Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to reduce the pDNA amou ...

American Chemical Society2015

Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

Transient gene expression (TGE) in human embryonic kidney (HEK-293) and Chinese hamster ovary (CHO) cells is a well-established technology for the rapid generation of recombinant proteins. Although the maximum TGE yields have reached 1 g/L or more, the amo ...

American Chemical Society2015

Transient Production of Recombinant Adeno-Associated Virus (AAV) Vectors for Gene Therapy Applications Using Suspension-Adapted HEK 293 Cells in Orbital Shaken Bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Nicole Déglon, Bernard Schneider, Monique Montavon, Daniel Roman Blessing

Nature Publishing Group2015

Arginine-Based Biodegradable Ether–Ester Polymers with Low Cytotoxicity as Potential Gene Carriers

Jutta Christine Wandrey, Lucia Baldi Unser, Sagar Shashidhar Manoli, Nino Zavradashvili, Tamar Memanishvili

The success of gene therapy depends on safe and effective gene carriers. Despite being widely used, synthetic vectors based on poly(ethylenimine) (PEI), poly(l-lysine) (PLL), or poly(l-arginine) (poly-Arg) are not yet fully satisfactory. Thus, both improve ...

American Chemical Society2014

Virus-free transient protein production in Sf9 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

A method for virus-free transient gene expression from suspension-adapted Sf9 insect cells was developed with the gene of interest being expressed from a plasmid carrying the homologous region 5 enhancer (hr5) and the immediate early 1 (ie1) promoter from ...

Elsevier2014

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

Florian Maria Wurm, Lucia Baldi Unser, Yashas Rajendra, Fatemeh Davami

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of ...

2014

Cell Compatible Arginine Containing Cationic Polymer: One-Pot Synthesis and Preliminary Biological Assessment

Jutta Christine Wandrey, Lucia Baldi Unser, Xiao Shen, Nino Zavradashvili, Tamar Memanishvili

Synthetic cationic polymers are of interest as both nonviral vectors for intracellular gene delivery and antimicrobial agents. For both applications synthetic polymers containing guanidine groups are of special interest since such kind of organic compounds ...

Springer India2014

Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Saroj Ghimire, Dominique Terry Monteil Jr, Giulia Tontodonati

Orbitally shaken bioreactors (OSRs) support the efficient cultivation of mammalian cells in suspension at various scales of operation. Here we introduce a disposable culture vessel with a ventilated cap having a nominal volume of 600 mL (TubeSpin (R) biore ...

Elsevier2013

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Stéphanie Tissot, Clara Joséphine Douet

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefﬁcient of oxygen (kLa) was 200%–400% higher in the HT tu ...

2013

Study of the improved Sf9 transient gene expression process

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TG ...

2013

Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sarah Thurnheer

Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfect ...

Elsevier2013

The optimization of a rapid low-cost alternative of large-scale medium sterilization

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Dominique Terry Monteil Jr

2013