Publications by Niels Erik Lion

Red blood cells ageing markers: a multi-parametric analysis

Gerardo Turcatti, Michel Prudent, Niels Erik Lion, Benjamin Rappaz, Manon Sandra Bardyn, David Crettaz

Background. Red blood cells collected in citrate-phosphate-dextrose can be stored for up to 42 days at 4 degrees C in saline-adenine-glucose-mannitol additive solution. During this controlled, but nevertheless artificial, ex vivo ageing, red blood cells ac ...

Simti Servizi Srl2017

The antioxidant capacity of erythrocyte concentrates is increased during the first week of storage and correlated with the uric acid level

Hubert Girault, Michel Prudent, Niels Erik Lion, Fernando Cortes Salazar, Manon Sandra Bardyn, Sunny Isaïe Maye, Andreas Stephan Lesch

Background and Objectives: Red blood cells (RBCs) suffer from lesions during cold storage, depending in part on their ability to counterbalance oxidative stress by activating their antioxidant defence. The aim of this study was to monitor the antioxidant p ...

Wiley-Blackwell2017

Sensitive and fast identification of bacteria in blood samples by immunoaffinity mass spectrometry for quick BSI diagnosis

Hubert Girault, Horst Pick, Michel Prudent, Niels Erik Lion, Liang Qiao, Natalia Gasilova, Alexandra Bondarenko, Yingdi Zhu

Bloodstream infections rank among the most serious causes of morbidity and mortality in hospitalized patients, partly due to the long period (up to one week) required for clinical diagnosis. In this work, we have developed a sensitive method to quickly and ...

Royal Soc Chemistry2016

Dynamic Metabolic Flux Analysis of Stored Red Blood Cells

Michel Prudent, Niels Erik Lion, Frédéric Stauber

Wiley-Blackwell2016

The Antioxidant Defense System of Erythrocyte Concentrates is Increased during the First Week of Storage

Michel Prudent, Niels Erik Lion, Michael Müller, Fernando Cortes Salazar

Wiley-Blackwell2016

Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy

Horst Vogel, Michel Prudent, Niels Erik Lion, Luigino Grasso, Romain Wyss, Davide Demurtas, Ashwin Thampi, Lorenz Weidenauer

We report on a generic method to detect and iden- tify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identit ...

Springer Heidelberg2015

Loss of Protein Tyr-phosphorylation During in vitro Storage of Human Erythrocytes: Impact on RBC Morphology

Gerardo Turcatti, Pierre Marquet, Michel Prudent, Niels Erik Lion, Benjamin Rappaz

Wiley-Blackwell2014

Large scale inkjet-printing of carbon nanotubes electrodes for antioxidant assays in blood bags

Hubert Girault, Michel Prudent, Niels Erik Lion, Fernando Cortes Salazar, Andreas Stephan Lesch

Herein, we present the large scale fabrication of carbon nanotubes (CNT) electrodes supported on flexible polymeric sheets by subsequent multilayer inkjet printing of a silver layer for electrical connection, CNT layers as active electrode material and an ...

Elsevier2014

Dual-Channel Electrospray Microchip

Hubert Girault, Niels Erik Lion, Yu Lu, Fang Liu

A dual-channel electrospray microchip has been developed for electrospray ionization mass spectrometry (ESI-MS) where aqueous samples are mixed at the Taylor cone with an organic buffer. Due to the fast and effective mixing in the Taylor cone, the aqueous ...

2013

Electrochemical reactions and ionization processes

Hubert Girault, Michel Prudent, Niels Erik Lion, Mélanie Abonnenc, Hongyan Bi, Liang Qiao

Electrochemical or photo-electrochemical reactions in both electrospray ionization and laser desorption ionization are discussed stressing the role of the electrode reaction in influencing the ionization process. In particular, upon application of a high v ...

2010

Plate for matrix-assisted laser desorption ionization mass spectrometry, comprises electroconductive substrate covered with sintered nanoparticles, deposited as array of spots, for supporting ionization of sorbed sample molecules

Hubert Girault, Michel Prudent, Niels Erik Lion, Christophe Roussel, Jingjing Wan, Liang Qiao

NOVELTY - A plate comprises an electroconductive substrate (1) at least partially covered with sintered nanoparticles (2), deposited as an array of individual spots, for use as a sorbing phase for a sample, and for supporting ionization of sorbed sample mo ...

2009

Iontophoretic Fraction Collection for Coupling Capillary Zone Electrophoresis with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Hubert Girault, Niels Erik Lion

An automated fraction collection interface has been developed for coupling capillary electrophoresis (CE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). This fraction collection approach is based on electromigration and diff ...

2009

About the Electrospray Ionization Source in Mass Spectrometry: Electrochemistry and On-chip Reactions

Hubert Girault, Joël Stéphane Rossier, Michel Prudent, Niels Erik Lion, Christophe Roussel, Bin Su, Manuel Alejandro Méndez Agudelo

The present work shows that the electrochemical properties of electrospray ionization (ESI) can be used to add functions to the process. As example, we show how the choice of the electrode material can be used to study interactions between metal ions and b ...

2009

Electrospray Micromixer Chip for On-Line Derivatization and Kinetic Studies

Hubert Girault, Niels Erik Lion, Loïc Dayon, Brice Perruche, Mélanie Abonnenc

An electrospray microchip for mass spectrometry comprising an integrated passive mixer to carry out on-chip chemical derivatizations is described. The microchip fabricated using UV-photoablation is composed of two microchannels linked together by a liquid ...

2008

Microfabricated Dual Sprayer for On-Line Mass Tagging of Phosphopeptides

Hubert Girault, Joël Stéphane Rossier, Michel Prudent, Niels Erik Lion

Phosphopeptides tagging reactions by dinuclear zinc(II) complexes (1,3-bis[bis(2-pyridylmethyl)amino]-propan- 2-olato dizinc(II)3+, called tag) were performed with a dual-channel microsprayer in electrospray ionization mass spectrometry. The reaction is fi ...

2008

Plate for matrix-assisted laser desorption ionization mass spectrometry for, e.g. analyzing peptide, has substrate that is covered with light sensitive matrix with light absorber, charge carrier, probe molecule and photo-sensitizer

Hubert Girault, Niels Erik Lion, Christophe Roussel, Liang Qiao

NOVELTY - The plate has electrically conductive substrate (1) that is covered with light sensitive matrix (2). The matrix comprises light absorber, charge carrier, probe molecule and photo-sensitizer (3) arranged to oxidize probe molecule by irradiation of ...

2008

Microparticles in stored red blood cells: an approach using flow cytometry and proteomic tools

Niels Erik Lion, David Crettaz, Olivier Rubin

Background and Objectives Microparticles (MPs) are small phospholipid vesicles of less than 1 mu m, shed in blood flow by various cell types. These MPs are involved in several biological processes and diseases. MPs have also been detected in blood products ...

Wiley-Blackwell2008

Modeling the Isoelectric Focusing of Peptides in an OFFGEL Multicompartment Cell

Hubert Girault, Niels Erik Lion, Hoang-Trang Lam

In proteomic analysis of complex samples at the peptide level (termed shotgun proteomics), an effective prefractionation is crucial to decrease the complexity of the peptide mixture for further analysis. In this perspective, the high-resolving power of the ...

American Chemical Society2007

Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation

Hubert Girault, Niels Erik Lion

Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum a ...

2007

Detection of proteins on membranes and in microchannels using copper staining combined with Scanning Electrochemical Microscopy

Hubert Girault, Niels Erik Lion

Scanning Electrochemical Microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained us ...

2007