Publications by Chia-Lung Hsieh

Tissue and cellular imaging

Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Xin Yang, Ye Pu

Systems and methods for imaging ear tissue include: directing illumination radiation to pass through an intact biological structure and be incident on ear tissue that does not include an exogenous fluorophore, at a plurality of locations, the illumination ...

2013

Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis

Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Xin Yang, Ye Pu

Sensorineural hearing loss is the most common type of hearing loss worldwide, yet the underlying cause is typically unknown because the inner ear cannot be biopsied today without destroying hearing, and intracochlear cells have not been imaged with resolut ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Three-dimensional scanning microscopy through thin turbid media

Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Xin Yang, Ye Pu

We demonstrate three-dimensional imaging through a thin turbid medium using digital phase conjugation of the second harmonic signal emitted from a beacon nanoparticle. The digitally phase-conjugated focus scans the volume in the vicinity of its initial pos ...

2012

Imaging with second-harmonic radiation probes in living tissue

Rachel Grange, Demetri Psaltis, Thomas Jean Victor Marie Lanvin, Chia-Lung Hsieh, Ye Pu, Ye Pu

We demonstrate that second-harmonic radiation imaging probes are efficient biomarkers for imaging in living tissue. We show that 100 nm and 300 nm BaTiO3 nanoparticles used as contrast markers could be detected through 50 mu m and 120 mu m of mouse tail ti ...

2011

Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Grégoire Pierre Jean Laporte, Ye Pu

We demonstrate imaging through a turbid layer by using digital phase conjugation of the second harmonic field radiated from a beacon nanoparticle. We show that the phase-conjugated focus can be displaced from its initial position by illuminating the same r ...

2010

Second harmonic generation from nanocrystals under linearly and circularly polarized excitations

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

We study second harmonic generation (SHG) from noncentrosymmetric nanocrystals under linearly polarized (LP) and circularly polarized (CP) excitations. Theoretical models are developed for SHG from nanocrystals under both plane-wave and focused excitations ...

2010

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Nonlinear optical properties of core-shell nanocavities for enhanced second-harmonic generation

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

A nonlinear optical plasmonic core-shell nanocavity is demonstrated as an efficient, subwavelength coherent light source through second-harmonic generation. The nonlinear optical plasmonic nanocavity incorporates a noncentrosymmetric medium, which utilizes ...

2010

Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging: erratum

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

An error was made in calculating the polarization dependent second harmonic response of barium titanate nanoparticles. We have corrected the error and repeated the comparison with the experimental results. (C) 2010 Optical Society of America ...

2010

Bioconjugation of barium titanate nanocrystals with immunoglobulin G antibody for second harmonic radiation imaging probes

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

The second harmonic generation (SHG) active nanocrystals have been demonstrated as attractive imaging probes in nonlinear microscopy due to their coherent, non-bleaching and non-blinking signals with a broad flexibility in the choice of excitation waveleng ...

Elsevier2010

Digital phase conjugation of second harmonic radiation emitted by nanoparticles in turbid media

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

We demonstrate focusing coherent light on a nanoparticle through turbid media based on digital optical phase conjugation of second harmonic generation (SHG) field from the nanoparticle. A SHG active nanoparticle inside a turbid medium was excited at the fu ...

2010

Harmonic Holography

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

2010

Lithium niobate nanowires: Growth, second-harmonic and dielectrophoretic properties

László Forró, Rachel Grange, Arnaud Magrez, Demetri Psaltis, Jae-Woo Choi, Chia-Lung Hsieh, Ye Pu, Ye Pu

2009

Harmonic holographic microscopy with circularly polarized excitation

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

We have developed a luminescent marker using the second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocrystals. These nanoparticles are attractive due to their stable, non-saturating and coherent signal with a femtosecond-scale response time ...

2009

Three-dimensional harmonic holographic microcopy using nanoparticles as probes for cell imaging

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocry ...

Optical Society of America2009

Lithium niobate nanowires synthesis, optical properties, and manipulation

László Forró, Rachel Grange, Arnaud Magrez, Demetri Psaltis, Jae-Woo Choi, Chia-Lung Hsieh, Rita Smajda, Ye Pu, Ye Pu

Free-standing lithium niobate nanowires (LiNbO3) are synthesized by the hydrothermal route. The polarization response of the second harmonic generation (SHG) signal is measured in a single nanowire and used to identify the crystal orientation by matching w ...

2009

Barium titanate nanoparticles used as second harmonic radiation imaging probes for cell imaging

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

Luminescent markers play a key role in imaging techniques for life science since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocry ...

2009

Ultrasensitive second harmonic generation nanoprobes via plasmonic coreshell structures

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

2009

Harmonic holography for three-dimensional cellular imaging

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

Luminescent markers play a key role in imaging techniques for life sciences since they provide a contrast mechanism between signal and background. We describe a new type of marker using second harmonic generation (SHG) from noncentrosymmetric BaTiO3 nanocr ...

2009

Second-harmonic efficiency of single barium titanate nanoparticles used as biomarkers for cells imaging

Rachel Grange, Demetri Psaltis, Chia-Lung Hsieh, Ye Pu, Ye Pu

2009