Publications by Pushpendra Singh

Population Genomics of Mycobacterium leprae Reveals a New Genotype in Madagascar and the Comoros

Stewart Cole, Philippe Busso, Pushpendra Singh, Stefanie Boy-Röttger, Andrej Benjak, Charlotte Avanzi, Thyago Leal Calvo

Human settlement of Madagascar traces back to the beginning of the first millennium with the arrival of Austronesians from Southeast Asia, followed by migrations from Africa and the Middle East. Remains of these different cultural, genetic, and linguistic ...

Frontiers Media SA2020

Ancient genomes reveal a high diversity of Mycobacterium leprae in medieval Europe

Stewart Cole, Pushpendra Singh, Andrej Benjak, Charlotte Avanzi

2018

Phylogenomics and antimicrobial resistance of the leprosy bacillus Mycobacterium leprae

Stewart Cole, Philippe Busso, Pushpendra Singh, Chloé Marie Loiseau, Andrej Benjak, Charlotte Avanzi, Andréanne Lupien

Nature Research2018

Ancient Mycobacterium leprae genomes reveal an unexpected diversity of leprosy in medieval Europe

Stewart Cole, Pushpendra Singh, Andrej Benjak, Charlotte Avanzi

WILEY2018

Whole genome sequencing distinguishes between relapse and reinfection in recurrent leprosy cases

Stewart Cole, Philippe Busso, Pushpendra Singh, Chloé Marie Loiseau, Andrej Benjak, Charlotte Avanzi, Jérémie Piton

Background Since leprosy is both treated and controlled by multidrug therapy (MDT) it is important to monitor recurrent cases for drug resistance and to distinguish between relapse and reinfection as a means of assessing therapeutic efficacy. All three obj ...

Public Library Science2017

Insight into the evolution and origin of leprosy bacilli from the genome sequence of Mycobacterium lepromatosis

Stewart Cole, Philippe Busso, Pushpendra Singh, Andrej Benjak, Charlotte Avanzi

Mycobacterium lepromatosis is an uncultured human pathogen associated with diffuse lepromatous leprosy and a reactional state known as Lucio's phenomenon. By using deep sequencing with and without DNA enrichment, we obtained the near-complete genome sequen ...

National Academy of Sciences2015

Mycobacterium lepromatosis Infections in Nuevo Leon, Mexico

Stewart Cole, Philippe Busso, Pushpendra Singh, Andrej Benjak, Charlotte Avanzi

The frequency of infection caused by the recently described pathogen Mycobacterium lepromatosis is unknown. Here, we describe the demographics, clinical characteristics, and therapeutic outcomes of five lepromatous leprosy patients suffering from M. leprom ...

American Society for Microbiology2015

Zoonotic Leprosy in the Southeastern United States

Stewart Cole, Pushpendra Singh, Rahul Sharma

Nine-banded armadillos (Dasypus novemcinctus) are naturally infected with Mycobacterium leprae and have been implicated in zoonotic transmission of leprosy. Early studies found this disease mainly in Texas and Louisiana, but armadillos in the southeastern ...

Centers Disease Control2015

Genome-wide re-sequencing of multidrug-resistant Mycobacterium leprae Airaku-3

Stewart Cole, Jacques Rougemont, Philippe Busso, Pushpendra Singh, Andrej Benjak, Charlotte Avanzi

Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug-resist ...

Wiley-Blackwell2014

Mycobacterium leprae genomes from a British medieval leprosy hospital: towards understanding an ancient epidemic

Stewart Cole, Pushpendra Singh

Background: Leprosy has afflicted humankind throughout history leaving evidence in both early texts and the archaeological record. In Britain, leprosy was widespread throughout the Middle Ages until its gradual and unexplained decline between the 14th and ...

Biomed Central Ltd2014

Detection and Strain Typing of Ancient Mycobacterium leprae from a Medieval Leprosy Hospital

Stewart Cole, Pushpendra Singh

Nine burials excavated from the Magdalen Hill Archaeological Research Project (MHARP) in Winchester, UK, showing skeletal signs of lepromatous leprosy (LL) have been studied using a multidisciplinary approach including osteological, geochemical and biomole ...

Public Library of Science2013

Genome-wide comparison of medieval and modern Mycobacterium leprae

Stewart Cole, Philippe Busso, Pushpendra Singh, Andrej Benjak

Leprosy was endemic in Europe until the Middle Ages. Using DNA array capture, we have obtained genome sequences of Mycobacterium leprae from skeletons of five medieval leprosy cases from the United Kingdom, Sweden, and Denmark. In one case, the DNA was so ...

American Association for the Advancement of Science2013

Molecular drug susceptibility testing and genotyping of Mycobacterium leprae strains from South America

Stewart Cole, Philippe Busso, Pushpendra Singh

Possible drug resistance in Mycobacterium leprae strains from Venezuela and three other South American countries was surveyed by molecular methods. None of the 230 strains from new leprosy cases exhibited drug resistance-associated mutations. However, two ...

2011

Probable zoonotic leprosy in the southern United States

Stewart Cole, Adamantia Kapopoulou, Jacques Rougemont, Philippe Busso, Pushpendra Singh, Rahul Sharma

In the southern region of the United States, such as in Louisiana and Texas, there are autochthonous cases of leprosy among native-born Americans with no history of foreign exposure. In the same region, as well as in Mexico, wild armadillos are infected wi ...

2011

A Case of Diffuse Lepromatous Leprosy associated with Mycobacterium lepromatosis

Stewart Cole, Philippe Busso, Pushpendra Singh

An 86-year old female patient from northeast Mexico presented with diffuse lepromatous leprosy (DLL). Sequence analysis of four genes (rrs, rpoB, sigA and hsp65) from the skin biopsy identified Mycobacterium lepromatosis. This is the first independent conf ...

2011

Mycobacterium leprae: genes, pseudogenes and genetic diversity

Stewart Cole, Pushpendra Singh

Leprosy, which has afflicted human populations for millenia, results from infection with Mycobacterium leprae, an unculturable pathogen with an exceptionally long generation time. Considerable insight into the biology and drug resistance of the leprosy bac ...

2010

Comparative genomic and phylogeographic analysis of Mycobacterium leprae

Stewart Cole, Jacques Rougemont, Alexandre Gattiker, Philippe Busso, Pushpendra Singh

Reductive evolution and massive pseudogene formation have shaped the 3.31-Mb genome of Mycobacterium leprae, an unculturable obligate pathogen that causes leprosy in humans. The complete genome sequence of M. leprae strain Br4923 from Brazil was obtained b ...

2009