Publications by Adrien Charles-François Raymond Descloux

High resolution optical projection tomography platform for multispectral imaging of the mouse gut

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Arielle Louise Planchette, Alessio Mylonas

Optical projection tomography (OPT) is a powerful tool for three-dimensional imaging of mesoscopic biological samples with great use for biomedical phenotyping studies. We present a fluorescent OPT platform that enables direct visualization of biological s ...

2021

Experimental Combination of Super-Resolution Optical Fluctuation Imaging with Structured Illumination Microscopy for Large Fields-of-View

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Suliana Manley, Dora Mahecic, Kristin Stefanie Grussmayer, Vytautas Navikas

All fluorescence super-resolution microscopy techniques present trade-offs between, for example, resolution, acquisition speed, and live-cell compatibility. Structured illumination microscopy (SIM) improves the resolution through successive imaging of the ...

AMER CHEMICAL SOC2021

Parameter-free rendering of single-molecule localization microscopy data for parameter-free resolution estimation

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer

Localization microscopy is a super-resolution imaging technique that relies on the spatial and temporal separation of blinking fluorescent emitters. These blinking events can be individually localized with a precision significantly smaller than the classic ...

2021

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Georg Fantner, Samuel Mendes Leitão, Kristin Stefanie Grussmayer, Barney Frederick Drake, Vytautas Navikas

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

Nature Research2021

Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer, Sanjin Marion, Vytautas Navikas

A variety of modern super-resolution micro-scopy methods provide researchers with previouslyinconceivable biological sample imaging opportunities ata molecular resolution. All of these techniques excel atimaging samples that are close to the coverslip, how ...

2021

Spectral cross-cumulants for multicolor super-resolved SOFI imaging

Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Tomas Lukes, Kristin Stefanie Grussmayer

Super-resolution opticalfluctuation imaging provides a resolution beyond the diffraction limitby analysing stochasticfluorescencefluctuations with higher-order statistics. Usingnthorderspatio-temporal cross-cumulants the spatial resolution and the sampling ...

Nature Research2020

Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

Adrien Charles-François Raymond Descloux

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

Benchmarking of single imaging datasets

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux

A method of estimating a value (311-313) indicative of a signal component of an input imaging dataset (190) includes determining a normalized Fourier-domain representation of the input imaging dataset (190); and for each one of multiple low-pass filters (3 ...

2020

High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Theo Lasser, Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Tomas Lukes, Vytautas Navikas

Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, ...

2020

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics

Theo Lasser, Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Tomas Lukes, Jochem Deen, Kristin Stefanie Grussmayer

Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to p ...

2019

Parameter-free image resolution estimation based on decorrelation analysis

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Kristin Stefanie Grussmayer

Super-resolution microscopy opened diverse new avenues of research by overcoming the resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual fluorophores made it possible to reveal structures beyond the diffraction l ...

2019

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Marcel Leutenegger, Theo Lasser, Hilal Lashuel, Adrien Charles-François Raymond Descloux, Emrah Bostan, Arno Pino Bouwens, Anne-Laure Mahul Mellier, Tomas Lukes

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Nature Publishing Group2018

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Séverine Coquoz, David Nguyen, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Miguel Sison

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Superresolved synthetic aperture microscope

Theo Lasser, Adrien Charles-François Raymond Descloux, Daniel Pawel Szlag, Tomas Lukes

Optical system comprising a synthetic aperture generating unit; said synthetic aperture generating unit being adapted for generating several apertures, which are correlated by interference for improving contrast and/or resolution based on the interference ...

2017

Aberrations of the point spread function of a multimode fiber due to partial mode excitation

Adrien Charles-François Raymond Descloux

We investigate the point spread function of a multimode fiber. The distortion of the focal spot created on the fiber output facet is studied for a variety of the parameters. We develop a theoretical model of wavefront shaping through a multimode fiber and ...

Optical Society of America2016