Publications by Arno Pino Bouwens

Identifying microbial species by single-molecule DNA optical mapping and resampling statistics

Theo Lasser, Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Tomas Lukes, Jochem Deen, Kristin Stefanie Grussmayer

Single-molecule DNA mapping has the potential to serve as a powerful complement to high-throughput sequencing in metagenomic analysis. Offering longer read lengths and forgoing the need for complex library preparation and amplification, mapping stands to p ...

2019

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Marcel Leutenegger, Theo Lasser, Hilal Lashuel, Adrien Charles-François Raymond Descloux, Emrah Bostan, Arno Pino Bouwens, Anne-Laure Mahul Mellier, Tomas Lukes

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Nature Publishing Group2018

Imaging of cortical structures and microvasculature using extended-focus optical coherence tomography at 1.3 mu m

Theo Lasser, Paul James Marchand, Damien Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag

2018

In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Daniel Pawel Szlag

SPIE Society of Photo-optical Instrumentation Engineers2018

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

Theo Lasser, Paul James Marchand, Séverine Coquoz, Johan Auwerx, Arno Pino Bouwens, Laurent Mouchiroud, Daniel Pawel Szlag, Vincenzo Sorrentino

Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the ne ...

Public Library of Science2017

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Séverine Coquoz, David Nguyen, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Miguel Sison

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Statistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Tristan Bolmont, David Nguyen, Vincent Khashayar Shamaei, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundre ...

Optical Soc Amer2017

Interferometric synthetic aperture microscopy for extended focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Séverine Coquoz, Arno Pino Bouwens, Jérôme Extermann

Optical coherence microscopy (OCM) is an interferometric technique providing 3D images of biological samples with micrometric resolution and penetration depth of several hundreds of micrometers. OCM differs from optical coherence tomography (OCT) in that i ...

2017

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Anne Grapin-Botton, Corinne Berclaz, Paul James Marchand, David Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers

Theo Lasser, Antonio Lopez, Anne Grapin-Botton, Corinne Berclaz, Christophe Pache, Arno Pino Bouwens, Daniel Pawel Szlag

The identification of a beta-cell tracer is a major quest in diabetes research. However, since MRI, PET and SPECT cannot resolve individual islets, optical techniques are required to assess the specificity of these tracers. We propose to combine Optical Co ...

Nature Publishing Group2015

IMAGING White-light diffraction tomography

Theo Lasser, Arno Pino Bouwens

Nature Publishing Group2014

Optical coherence correlation spectroscopy (OCCS)

Marcel Leutenegger, Theo Lasser, Stéphane Broillet, Stefan Geissbühler, Christophe Pache, Arno Pino Bouwens, Akihiro Sato

We present a new method called optical coherence correlation spectroscopy (OCCS) using nanoparticles as reporters of kinetic processes at the single particle level. OCCS is a spectral interferometry based method, thus giving simultaneous access to several ...

Optical Society of America2014

Visible light optical coherence correlation spectroscopy

Heinrich Hofmann, Marcel Leutenegger, Theo Lasser, Stéphane Broillet, Arno Pino Bouwens, Lionel André Maurizi, Daniel Pawel Szlag

Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a fact ...

Optical Society of America2014

Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

Theo Lasser, Corinne Berclaz, Tristan Bolmont, Arno Pino Bouwens, Daniel Pawel Szlag

Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line ...

Optical Society of America2014

Functional and Structural Imaging with Optical Coherence Microscopy : Principles & Applications

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

Quantitative lateral and axial flow imaging with optical coherence microscopy and tomography

Theo Lasser, Tristan Bolmont, Arno Pino Bouwens, Daniel Pawel Szlag

Optical coherence tomography (OCT) and optical coherence microscopy (OCM) allow the acquisition of quantitative three-dimensional axial flow by estimating the Doppler shift caused by moving scatterers. Measuring the velocity of red blood cells is currently ...

Optical Society of America2013

Diabetes imaging — quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Anthony Christopher Davison, Theo Lasser, Martin Villiger, Dimitri Nestor Alice Van De Ville, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Erica Martin-Williams, Arno Pino Bouwens

Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Cohere ...

2012

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Christian Santschi, Theo Lasser, Martin Villiger, Corinne Berclaz, Christophe Pache, Matthew Gibson, Joan Goulley, Noelia Laura Bocchio, Arno Pino Bouwens

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Zero-crossing approach to high-resolution reconstruction in frequency-domain optical-coherence tomography

Marcel Leutenegger, Theo Lasser, Chandra Sekhar Seelamantula, Arno Pino Bouwens

We address the problem of high-resolution reconstruction in frequency-domain optical-coherence tomography (FDOCT). The traditional method employed uses the inverse discrete Fourier transform, which is limited in resolution due to the Heisenberg uncertainty ...

2012