Publications by Dora Mahecic | EPFL Graph Search

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Dora Mahecic, Juliette Griffie, Chen Zhang, Martin Weigert, Willi Leopold Stepp

CELL PRESS2023

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Dora Mahecic, Juliette Griffie, Chen Zhang, Martin Weigert, Willi Leopold Stepp

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

2022

Flipper Probes for the Community

Suliana Manley, Dora Mahecic

This article describes four fluorescent membrane tension probes that have been designed, synthesized, evaluated, commercialized and applied to current biology challenges in the context of the NCCR Chemical Biology. Their names are Flipper-TR (R), ER Flippe ...

SWISS CHEMICAL SOC2021

Mitochondrial membrane tension governs fission

Suliana Manley, Lina Carlini, Dora Mahecic, Tatjana Isabelle Kleele

During mitochondrial fission, key molecular and cellular factors assemble on the outer mitochondrial membrane, where they coordinate to generate constriction. Constriction sites can eventually divide or reverse upon disassembly of the machinery. However, a ...

CELL PRESS2021

Experimental Combination of Super-Resolution Optical Fluctuation Imaging with Structured Illumination Microscopy for Large Fields-of-View

Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Suliana Manley, Dora Mahecic, Kristin Stefanie Grussmayer, Vytautas Navikas

All fluorescence super-resolution microscopy techniques present trade-offs between, for example, resolution, acquisition speed, and live-cell compatibility. Structured illumination microscopy (SIM) improves the resolution through successive imaging of the ...

AMER CHEMICAL SOC2021

Distinct fission signatures predict mitochondrial degradation or biogenesis

Hélène Danielle Perreten Lambert, Suliana Manley, Dora Mahecic, Tatjana Isabelle Kleele, Timo Henry René Rey, Julius Hans Bernd Winter

Mitochondrial fission is a highly regulated process that, when disrupted, can alter metabolism, proliferation and apoptosis(1-3). Dysregulation has been linked to neurodegeneration(3,4), cardiovascular disease(3) and cancer(5). Key components of the fissio ...

2021

Characterization of flat-fielding systems for quantitative microscopy

Suliana Manley, Dora Mahecic, Khalid Ashraf Mohie Ibrahim

Optical flat-fielding systems, such as field-mapping or integration-based beam shapers, are used to transform nonuniform illumination into uniform illumination. Thus, flat-fielding paves the way for imaging that is independent of position within a field of ...

2020

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Pierre Gönczy, Virginie Hamel, Suliana Manley, Paul Guichard, Dora Mahecic, Kyle Michael Douglass, Denis Fortun, Niccolo Banterle, Khalid Ashraf Mohie Ibrahim, Davide Gambarotto

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

2020

Koehler integrator device and application thereof in a multi-focal confocal microscope

Suliana Manley, Dora Mahecic, Kyle Michael Douglass

A Koehler integrator device (10) comprises a collimating lens (11) being arranged for collimating a light field created by an incoherent or partially coherent light source, a pair of planar first and second micro-lens arrays (12, 13) being arranged for rel ...

2020

High-throughput and adaptive super-resolution fluorescence microscopy of organelles

Dora Mahecic

Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...

EPFL2020

Mechanosensitive Fluorescent Probes to Image Membrane Tension in Mitochondria, Endoplasmic Reticulum, and Lysosomes

Stefan Matile, Suliana Manley, Dora Mahecic

Measuring forces inside cells is particularly challenging. With the development of quantitative microscopy, fluorophores which allow the measurement of forces became highly desirable. We have previously introduced a mechanosensitive flipper probe, which re ...

AMER CHEMICAL SOC2019

Strategies for increasing the throughput of super-resolution microscopies

Suliana Manley, Dora Mahecic, Juliette Griffie

Fluorescence microscopy methods have been developed to circumvent the diffraction limit by exploiting nonlinearities in the interactions between light and fluorophores. Initially, these methods were up to orders of magnitude slower than standard microscopi ...

ELSEVIER SCI LTD2019