Publications by Daniel Pawel Szlag

Imaging of cortical structures and microvasculature using extended-focus optical coherence tomography at 1.3 mu m

Theo Lasser, Paul James Marchand, Damien Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag

2018

In vivo high-resolution cortical imaging with extended-focus optical coherence microscopy in the visible-NIR wavelength range

Theo Lasser, Paul James Marchand, Arno Pino Bouwens, Daniel Pawel Szlag

SPIE Society of Photo-optical Instrumentation Engineers2018

Massively parallel data processing for quantitative total flow imaging with optical coherence microscopy and tomography

Theo Lasser, Paul James Marchand, Daniel Pawel Szlag, Marcin Antoni Sylwestrzak

We present an application of massively parallel processing of quantitative flow measurements data acquired using spectral optical coherence microscopy (SOCM). The need for massive signal processing of these particular datasets has been a major hurdle for m ...

Elsevier2017

Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

Theo Lasser, Paul James Marchand, Séverine Coquoz, Johan Auwerx, Arno Pino Bouwens, Laurent Mouchiroud, Daniel Pawel Szlag, Vincenzo Sorrentino

Fast, label-free, high-resolution, three-dimensional imaging platforms are crucial for high-throughput in vivo time-lapse studies of the anatomy of Caenorhabditis elegans, one of the most commonly used model organisms in biomedical research. Despite the ne ...

Public Library of Science2017

Visible spectrum extended-focus optical coherence microscopy for label-free sub-cellular tomography

Theo Lasser, Paul James Marchand, Séverine Coquoz, David Nguyen, Adrien Charles-François Raymond Descloux, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Miguel Sison

We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from ...

2017

Superresolved synthetic aperture microscope

Theo Lasser, Adrien Charles-François Raymond Descloux, Daniel Pawel Szlag, Tomas Lukes

Optical system comprising a synthetic aperture generating unit; said synthetic aperture generating unit being adapted for generating several apertures, which are correlated by interference for improving contrast and/or resolution based on the interference ...

2017

Statistical parametric mapping of stimuli evoked changes in total blood flow velocity in the mouse cortex obtained with extended-focus optical coherence microscopy

Theo Lasser, Paul James Marchand, Tristan Bolmont, David Nguyen, Vincent Khashayar Shamaei, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Functional magnetic resonance (fMRI) imaging is the current gold-standard in neuroimaging. fMRI exploits local changes in blood oxygenation to map neuronal activity over the entire brain. However, its spatial resolution is currently limited to a few hundre ...

Optical Soc Amer2017

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Anne Grapin-Botton, Corinne Berclaz, Paul James Marchand, David Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic, Lely Feletti, Daniel Pawel Szlag, Azat Sharipov, Hendrik Georges Deschout, Tomas Lukes

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Imaging of the stroke-related changes in the vascular system of the mouse brain with the use of extended focus Optical Coherence Microscopy

Theo Lasser, Daniel Pawel Szlag

We used Optical Coherence Microscopy (OCM) to monitor structural and functional changes due to ischemic stroke in small animals brains in vivo. To obtain lateral resolution of 2.2 μm over the range of 600 μm we used extended focus configuration of OCM inst ...

SPIE digital Library2016

Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers

Theo Lasser, Antonio Lopez, Anne Grapin-Botton, Corinne Berclaz, Christophe Pache, Arno Pino Bouwens, Daniel Pawel Szlag

The identification of a beta-cell tracer is a major quest in diabetes research. However, since MRI, PET and SPECT cannot resolve individual islets, optical techniques are required to assess the specificity of these tracers. We propose to combine Optical Co ...

Nature Publishing Group2015

Visible light optical coherence correlation spectroscopy

Heinrich Hofmann, Marcel Leutenegger, Theo Lasser, Stéphane Broillet, Arno Pino Bouwens, Lionel André Maurizi, Daniel Pawel Szlag

Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a fact ...

Optical Society of America2014

Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

Theo Lasser, Corinne Berclaz, Tristan Bolmont, Arno Pino Bouwens, Daniel Pawel Szlag

Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line ...

Optical Society of America2014

Quantitative lateral and axial flow imaging with optical coherence microscopy and tomography

Theo Lasser, Tristan Bolmont, Arno Pino Bouwens, Daniel Pawel Szlag

Optical coherence tomography (OCT) and optical coherence microscopy (OCM) allow the acquisition of quantitative three-dimensional axial flow by estimating the Doppler shift caused by moving scatterers. Measuring the velocity of red blood cells is currently ...

Optical Society of America2013