Scanning transmission electron microscopyA scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm]. As with a conventional transmission electron microscope (CTEM), images are formed by electrons passing through a sufficiently thin specimen. However, unlike CTEM, in STEM the electron beam is focused to a fine spot (with the typical spot size 0.05 – 0.2 nm) which is then scanned over the sample in a raster illumination system constructed so that the sample is illuminated at each point with the beam parallel to the optical axis.
Transmission electron microscopyTransmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device.
Electron diffractionElectron diffraction refers to changes in the direction of electron beams due to interactions with atoms. Close to the atoms the changes are described as Fresnel diffraction; far away they are called Fraunhofer diffraction. The resulting map of the directions of the electrons far from the sample (Fraunhofer diffraction) is called a diffraction pattern, see for instance Figure 1. These patterns are similar to x-ray and neutron diffraction patterns, and are used to study the atomic structure of gases, liquids, surfaces and bulk solids.
Cryogenic electron microscopyCryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution.
Electron microscopeAn electron microscope is a microscope that uses a beam of electrons as a source of illumination. They use electron optics that are analogous to the glass lenses of an optical light microscope. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light, electron microscopes have a higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes.
Charge-coupled deviceA charge-coupled device (CCD) is an integrated circuit containing an array of linked, or coupled, capacitors. Under the control of an external circuit, each capacitor can transfer its electric charge to a neighboring capacitor. CCD sensors are a major technology used in digital imaging. In a CCD , pixels are represented by p-doped metal–oxide–semiconductor (MOS) capacitors.
Video cameraA video camera is an optical instrument that captures videos (as opposed to a movie camera, which records images on film). Video cameras were initially developed for the television industry but have since become widely used for a variety of other purposes. Video cameras are used primarily in two modes. The first, characteristic of much early broadcasting, is live television, where the camera feeds real time images directly to a screen for immediate observation.
Aberration (astronomy)In astronomy, aberration (also referred to as astronomical aberration, stellar aberration, or velocity aberration) is a phenomenon which produces an apparent motion of celestial objects about their true positions, dependent on the velocity of the observer. It causes objects to appear to be displaced towards the direction of motion of the observer compared to when the observer is stationary. The change in angle is of the order of v/c where c is the speed of light and v the velocity of the observer.
Professional video cameraA professional video camera (often called a television camera even though its use has spread beyond television) is a high-end device for creating electronic moving images (as opposed to a movie camera, that earlier recorded the images on film). Originally developed for use in television studios or with outside broadcast trucks, they are now also used for music videos, direct-to-video movies (see digital movie camera), corporate and educational videos, wedding videos, among other uses.
Scanning electron microscopeA scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the surface topography and composition of the sample. The electron beam is scanned in a raster scan pattern, and the position of the beam is combined with the intensity of the detected signal to produce an image.
Transmission electron cryomicroscopyTransmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.
Image sensorAn image sensor or imager is a sensor that detects and conveys information used to form an . It does so by converting the variable attenuation of light waves (as they pass through or reflect off objects) into signals, small bursts of current that convey the information. The waves can be light or other electromagnetic radiation. Image sensors are used in electronic imaging devices of both analog and digital types, which include digital cameras, camera modules, camera phones, optical mouse devices, medical imaging equipment, night vision equipment such as thermal imaging devices, radar, sonar, and others.
Lorentz ether theoryWhat is now often called Lorentz ether theory (LET) has its roots in Hendrik Lorentz's "theory of electrons", which marked the end of the development of the classical aether theories at the end of the 19th and at the beginning of the 20th century. Lorentz's initial theory was created between 1892 and 1895 and was based on removing assumptions about aether motion. It explained the failure of the negative aether drift experiments to first order in v/c by introducing an auxiliary variable called "local time" for connecting systems at rest and in motion in the aether.
History of Lorentz transformationsThe history of Lorentz transformations comprises the development of linear transformations forming the Lorentz group or Poincaré group preserving the Lorentz interval and the Minkowski inner product . In mathematics, transformations equivalent to what was later known as Lorentz transformations in various dimensions were discussed in the 19th century in relation to the theory of quadratic forms, hyperbolic geometry, Möbius geometry, and sphere geometry, which is connected to the fact that the group of motions in hyperbolic space, the Möbius group or projective special linear group, and the Laguerre group are isomorphic to the Lorentz group.
Video camera tubeVideo camera tubes were devices based on the cathode ray tube that were used in television cameras to capture television images, prior to the introduction of charge-coupled device (CCD) in the 1980s. Several different types of tubes were in use from the early 1930s, and as late as the 1990s. In these tubes, an electron beam was scanned across an image of the scene to be broadcast focused on a target. This generated a current that was dependent on the brightness of the image on the target at the scan point.
Image resolutionImage resolution is the level of detail an holds. The term applies to digital images, film images, and other types of images. "Higher resolution" means more image detail. Image resolution can be measured in various ways. Resolution quantifies how close lines can be to each other and still be visibly resolved. Resolution units can be tied to physical sizes (e.g. lines per mm, lines per inch), to the overall size of a picture (lines per picture height, also known simply as lines, TV lines, or TVL), or to angular subtense.
Lorentz transformationIn physics, the Lorentz transformations are a six-parameter family of linear transformations from a coordinate frame in spacetime to another frame that moves at a constant velocity relative to the former. The respective inverse transformation is then parameterized by the negative of this velocity. The transformations are named after the Dutch physicist Hendrik Lorentz.
Spatial resolutionIn physics and geosciences, the term spatial resolution refers to distance between independent measurements, or the physical dimension that represents a pixel of the image. While in some instruments, like cameras and telescopes, spatial resolution is directly connected to angular resolution, other instruments, like synthetic aperture radar or a network of weather stations, produce data whose spatial sampling layout is more related to the Earth's surface, such as in remote sensing and .
Aether drag hypothesisIn the 19th century, the theory of the luminiferous aether as the hypothetical medium for the propagation of light waves was widely discussed. The aether hypothesis arose because physicists of that era could not conceive of light waves propagating without a physical medium in which to do so. When experiments failed to detect the hypothesized luminiferous aether, physicists conceived explanations, which preserved the hypothetical aether's existence, for the experiments' failure to detect it.
Velocity-addition formulaIn relativistic physics, a velocity-addition formula is an equation that specifies how to combine the velocities of objects in a way that is consistent with the requirement that no object's speed can exceed the speed of light. Such formulas apply to successive Lorentz transformations, so they also relate different frames. Accompanying velocity addition is a kinematic effect known as Thomas precession, whereby successive non-collinear Lorentz boosts become equivalent to the composition of a rotation of the coordinate system and a boost.
