Publications related to Protein-functionalized polymer brushes

Compliance-matching vascular grafts with personalization potential

Aortic diseases are characterized by dire prognosis and inadequate diagnosis, owing to their insidious yet lethal nature. Aortopathies, namely aortic aneurysms and dissections, along with certain congenital diseases, might necessitate surgical replacement ...

EPFL2023

Development of microfluidic tools and methods for the biochemical characterization of scFv and Cas9 affinity reagents and their applications in molecular detection

Ekaterina Emilova Petrova

Recent progress in protein engineering, empowered by the rapidly evolving fields of synthetic biology and DNA synthesis, has enabled the generation of various custom-designed proteins. Their thorough analysis has led to applications in areas including medi ...

EPFL2018

Surface-initiated controlled radical polymerization enhanced DNA biosensing

Harm-Anton Klok, Tugba Bilgiç Tune

The surface chemistry at the DNA recognition and binding interface plays an important role in determining the performance of DNA microarrays and DNA biosensors. Surface-initiated controlled radical polymerization (SI-CRP) reactions represent a powerful too ...

2015

Oligonucleotide Immobilization and Hybridization on Aldehyde-Functionalized Poly(2-hydroxyethyl methacrylate) Brushes

Harm-Anton Klok, Tugba Bilgiç Tune

DNA biosensing requires high oligonudeotide binding capacity interface chemistries that can be tuned to maximize probe presentation as well as hybridization efficiency. This contribution investigates the feasibility of aldehyde-functionalized poly(2-hydrox ...

American Chemical Society2015

Characterization of Three Amino-Functionalized Surfaces and Evaluation of Antibody Immobilization for the Multiplex Detection of Tumor Markers Involved in Colorectal Cancer

Nicolas Xanthopoulos, Vincent Laporte

Antibody microarrays are powerful and high-throughput tools for screening and identifying tumor markers from small sample volumes of only a few microliters. Optimization of surface chemistry and spotting conditions are crucial parameters to enhance antibod ...

Amer Chemical Soc2013

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation

Kai Johnsson

While antibodies currently play a dominant role as affinity reagents in biological research and for diagnostics, a broad range of recombinant proteins are emerging as promising alternative affinity reagents in detection assays and quantification. DNA-media ...

Elsevier2013

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sagar Shashidhar Manoli

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

Next generation microfluidic platforms for high-throughput protein biochemistry

Sebastian Maerkl

DNA technologies such as cloning, DNA microarrays, and next generation sequencing have transformed the life sciences. Protein technologies on the other hand have not seen such explosive progress. This is mainly due to the inherent difficulty of working wit ...

Elsevier2011

Interactive Surfaces Based on Polymer Brushes

Dusko Paripovic

While the bulk structure of a material determines its mechanical properties, its surface plays a key role when interactions with the environment are important. Nowadays materials in bio- and nanotechnology are required to possess very complex functionaliti ...

EPFL2011

Protein Microarrays Based on Polymer Brushes Prepared via Surface-Initiated Atom Transfer Radical Polymerization

Raphaël Barbey, Markus Ehrat, Harm-Anton Klok

Polymer brushes represent an interesting platform for the development of high-capacity protein binding surfaces. Whereas the protein binding properties of polymer brushes have been investigated before, this manuscript evaluates the feasibility of poly(glyc ...

American Chemical Society2010

Polymer Brushes as Substrates for Microarray Applications

Raphaël Barbey

The development of innovative bioanalytical technologies that enable the discovery of new therapeutics or the creation of systems for rapid, accurate and parallel analyses has gained an extraordinary interest in the biomedical sector in the last 10-15 year ...

EPFL2010

Room Temperature, Aqueous Post-Polymerization Modification of Glycidyl Methacrylate-Containing Polymer Brushes Prepared via Surface-Initiated Atom Transfer Radical Polymerization

Raphaël Barbey, Harm-Anton Klok

This manuscript reports on the post-polymerization modification of poly(glycidyl methacrylate) (PGMA) and PGMA-co-poly(2-(diethylamino)ethyl methacrylate) (PGMA(x)-co-PDEAEMA(y)) (co)polymer brushes prepared via surface-initiated atom transfer radical poly ...

2010

Protein-binding microarray technology and ChIP-sequencing for the identification of novel targets of the human tumor suppressor AP2[alpha]

Jan Kerschgens

The mis-regulation of gene transcription in the living cell can be the cause of complex human diseases like cancer. One challenge of the post-genomic era of biomedical research is the development of in vitro techniques for the rapid and cost-effective anal ...

EPFL2009

Biofunctional and biomimetic polymer brushes prepared via surface-initiated atom transfer radical polymerization

Harm-Anton Klok

Surface-initiated controlled radical polymerization is a powerful strategy to tailor the chemical and physical surface properties of materials. This article highlights recent work from the author's laboratory in which surface-initiated atom transfer radica ...

2008

Functional polymer brushes via surface-initiated controlled radical polymerizations

Stefano Tugulu

The term polymer brush refers to a well defined arrangement of polymer chains, which are tethered with one end to an interface and usually the surface of a solid substrate. Due to steric repulsion the polymer chains furthermore adopt a defined, stretched c ...

EPFL2007

Chemical approaches to microarrays and multifunctional protein labeling

India Christina Sielaff

With the human genome and many other eukaryotic and procaryotic genomes sequenced, the interest in life sciences is shifting towards the examination of the function, modification and regulation of the encoded proteins. Proteins are involved in virtually al ...

EPFL2006

Protein function microarrays based on self-immobilizing and self-labeling fusion proteins

Kai Johnsson, India Christina Sielaff, Harm-Anton Klok, Anke Arnold, Stefano Tugulu

Protein microarrays are an attractive approach for the high-throughput analysis of protein function, but their impact on proteomics has been limited by the technical difficulties associated with their generation. Here we demonstrate that fusion proteins of ...

2006

Protein-Functionalized Polymer Brushes

Kai Johnsson, India Christina Sielaff, Harm-Anton Klok, Anke Arnold, Stefano Tugulu

A new strategy for the prepn. of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymn. (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subse ...

American Chemical Society2005