Publications associées à Sphingolipide

Structural diversity of photoswitchable sphingolipids for optodynamic control of lipid microdomains

Sphingolipids are a structurally diverse class of lipids predominantly found in the plasma membrane of eukaryotic cells. These lipids can laterally segregate with other rigid lipids and cholesterol into liquid-ordered domains that act as organizing centers ...

2023

Inhibiting de novo ceramide synthesis restores mitochondrial and protein homeostasis in muscle aging

Johan Auwerx, Xiaoxu Li, Mario Romani, Tanes Imamura de Lima, Sandra Rodriguez Lopez, Hao Li, Martin Rainer Wohlwend, Pirkka-Pekka Untamo Laurila, Ludger Jan Elzuë Goeminne, Dohyun Park, Barbara Moreira Crisol, Changmyung Oh, Jean-David Horacio Morel

Disruption of mitochondrial function and protein homeostasis plays a central role in aging. However, how these processes interact and what governs their failure in aging remain poorly understood. Here, we showed that cer- amide biosynthesis controls the de ...

AMER ASSOC ADVANCEMENT SCIENCE2023

Inhibition of CERS1 in skeletal muscle exacerbates age-related muscle dysfunction

Johan Auwerx, Olivier Burri, Xiaoxu Li, Tanes Imamura de Lima, Giacomo Vincenzo Giorgio Von Alvensleben, Martin Rainer Wohlwend, Pirkka-Pekka Untamo Laurila, Ludger Jan Elzuë Goeminne, Amélia Lalou, Barbara Moreira Crisol, Renata Mangione

Age-related muscle wasting and dysfunction render the elderly population vulnerable and incapacitated, while underlying mechanisms are poorly understood. Here, we implicate the CERS1 enzyme of the de novo sphingolipid synthesis pathway in the pathogenesis ...

2023

Sphingolipids accumulate in aged muscle, and their reduction counteracts sarcopenia

Johan Auwerx, Davide D'Amico, Tanes Imamura de Lima, Qi Wang, Sébastien Robert Victor Herzig, Maroun Bou Sleiman, Martin Rainer Wohlwend, Peiling Luan, Pirkka-Pekka Untamo Laurila, Barbara Moreira Crisol

Age-related muscle dysfunction and sarcopenia are major causes of physical incapacitation in older adults and currently lack viable treatment strategies. Here we find that sphingolipids accumulate in mouse skeletal muscle upon aging and that both genetic a ...

2022

Type 2 diabetes disrupts circadian orchestration of lipid metabolism and membrane fluidity in human pancreatic islets

Howard Riezman, Jonathan Paz Montoya

Recent evidence suggests that circadian clocks ensure temporal orchestration of lipid homeostasis and play a role in pathophysiology of metabolic diseases in humans, including type 2 diabetes (T2D). Nevertheless, circadian regulation of lipid metabolism in ...

PUBLIC LIBRARY SCIENCE2022

Second harmonic scattering of water in a biological context

Tereza Schönfeldová

Water is one of the most abundant molecules in the universe. It forms a hydrogen bond network with unique structure and dynamics. Hydrogen bonding is highly relevant to many biological processes including membrane formation, protein folding, adsorption, an ...

EPFL2022

Single-cell lipidomics reveals a role for cell-to-cell sphingolipid heterogeneity in fibroblast activation

Laura Capolupo

Cell-to-cell variability plays a key role in tissue patterning by setting initial asymmetry that primes cell fate decisions. Fluctuations in the activity of regulatory molecules can commit individual cells to divergent differentiation pathways, and cell-to ...

EPFL2021

Golgi maturation-dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3

Giovanni D'Angelo, Laura Capolupo, Anthony Vocat, Riccardo Rizzo

Glycosphingolipids are important components of the plasma membrane where they modulate the activities of membrane proteins including signalling receptors. Glycosphingolipid synthesis relies on competing reactions catalysed by Golgi-resident enzymes during ...

WILEY2021

Treatment and prevention of aging related-disease and/or aging by the inhibition of sphingolipids

Johan Auwerx, Pirkka-Pekka Untamo Laurila

The present invention relates to an inhibitor of sphingolipids for use in the treatment or prevention of an aging related-disease and/or aging. ...

2021

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Florence Pojer, Maria Josefina Marcaida Lopez, Sylvia Ho, Francisco De Magalhães Sarmento R De Mesquita, Muhammad Umair Anwar, Patrick Alain Sandoz, Martina Audagnotto, Giulia Fonti

Acyl protein thioesterase APT2 interacts with membranes via its charged beta-tongue, becomes palmitoylated by ZDHHC3/7 and deforms the bilayer to extract substrate acyl chains. APT2 deacylation leads to its membrane release and degradation. ...

NATURE RESEARCH2021

Metabolic rescue of muscle and muscle stem cells in muscle disease

Peiling Luan

Duchenne muscular dystrophy (DMD), caused by the mutation of dystrophin gene, is an X-linked disorder that affects 1 in 3500 males, leading to progressive muscle degeneration and eventually resulting in premature death. Increasing evidence indicates that D ...

EPFL2020

Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

Oriented cell division is a fundamental mechanism to control asymmetric stem cell division, neural tube elongation and body axis extension, among other processes. During zebrafish gastrulation, when the body axis extends, dorsal epiblast cells display divi ...

Nature Publishing Group2020

Meeting Report – The 2019 FEBS special meeting on sphingolipid biology: sphingolipids in physiology and pathology

Giovanni D'Angelo

Sphingolipids are a fundamental class of molecules that are involved in structural, organizational and signaling properties of eukaryotic membranes. Defects in their production or disposal lead to acquired and inherited human diseases. A growing community ...

2019

Visualizing sphingolipid biosynthesis in cells

Biosynthetic pathways play a fundamental role in the building and operation of the cell by synthesizing the constituents by which the cell is constructed, and by producing signalling intermediates that play a key role in cell regulation. While a lot is kno ...

2019

A new class of protein biomarkers based on subcellular distribution: application to a mouse liver cancer model

Kristina Schoonjans, Vera Monica Lemos Da Silva, Evan Graehl Williams, Pan Xu, Amir Banaei Esfahani, Chen Li, Ruedi Aebersold

To-date, most proteomic studies aimed at discovering tissue-based cancer biomarkers have compared the quantity of selected proteins between case and control groups. However, proteins generally function in association with other proteins to form modules loc ...

Nature Research2019

Glycosphingolipid metabolism in cell fate specification

Giovanni D'Angelo, Laura Capolupo, Jaipreet Singh Loomba

Glycosphingolipids (GSLs) are ubiquitous components of eukaryotic plasma membranes that consist of a ceramide backbone linked to a glycan moiety. Both the ceramide and the glycan parts of GSLs display structural variations that result in a remarkable reper ...

2018

Mitochondria-specific photoactivation to monitor local sphingosine metabolism and function

Howard Riezman

Photoactivation ('uncaging') is a powerful approach for releasing bioactive small molecules in living cells. Current uncaging methods are limited by the random distribution of caged molecules within cells. We have developed a mitochondria-specific photoact ...

2018

Polycation Interactions with Zwitterionic Phospholipid Monolayers on Oil Nanodroplets Suspensions in Water (D2O) Probed by Sum Frequency Scattering

Sylvie Roke, Nikolay Smolentsev

By combining dynamic light scattering (DLS) measurements and infrared (IR) spectroscopy with the interface- and bond-specificity of vibrational sum frequency generation scattering (SFS) spectroscopy, we probe several structural aspects of how zwitterionic ...

2018

Studying membrane-protein interplay by modeling realistic conditions

Martina Audagnotto

Realistic models of cellular environments have long captured the imagination of biologists and physical scientists. More than 20 years ago, Goodshellâ s inspiring rendering of cellular environments provided the foundation for the idea of capturing the ful ...

EPFL2017

A method for analysis and design of metabolism using metabolomics data and kinetic models: Application on lipidomics using a novel kinetic model of sphingolipid metabolism

Vassily Hatzimanikatis, Paolo Angelino, Howard Riezman, Georgios Savoglidis

We present a model-based method, designated Inverse Metabolic Control Analysis (IMCA), which can be used in conjunction with classical Metabolic Control Analysis for the analysis and design of cellular metabolism. We demonstrate the capabilities of the met ...

Academic Press Inc Elsevier Science2016