Publications associées à Transitional epithelium

Identification of genes required for host-cell adhesion and antibiotic persistence of uropathogenic Escherichia coli via high-content screening

Uropathogenic Escherichia coli (UPEC) are the most common bacterial pathogens causing urinary tract infections (UTIs). Driven by the development of antibiotic resistant UPEC strains, UTIs have become a major public health issue and generate substantial hea ...

EPFL2022

Microtissue models for studying the dynamics of host-pathogen interactions in the urinary bladder

Kunal Sharma

Urinary tract infections (UTIs) are amongst the most common bacterial infections and are the second-most frequent reason for antibiotic prescriptions. Moreover, in about 25% of all treated cases, recurrence of infection occurs. Uropathogenic Escherichia co ...

EPFL2021

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection

John McKinney, Graham Knott, Neeraj Dhar, François Signorino-Gelo, Vivek Vijay Thacker, Kunal Sharma, Thomas Marie Simonet

Uropathogenic Escherichia coli (UPEC) proliferate within superficial bladder umbrella cells to form intracellular bacterial communities (IBCs) during early stages of urinary tract infections. However, the dynamic responses of IBCs to host stresses and anti ...

2021

Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Mouti Elodie Mouloungui

Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaff ...

Mary Ann Liebert, Inc2015

Clonal, Self-Renewing and Differentiating Human and Porcine Urothelial Cells, a Novel Stem Cell Population

Yann Barrandon, Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, François Gorostidi

Although urothelial progenitor-like cells have been described in the human urinary tract, the existence of stem cells remains to be proven. Using a culture system that favors clonogenic epithelial cell growth, we evaluated and characterized clonal human ur ...

Public Library of Science2014

Culture and Characterization of Human and Porcine Urothelial Progenitor Cells

Hans Mattias Larsson

An estimated 400 million people worldwide suffer from bladder diseases. However, little is known at the cellular level as to what causes or initiates many of these diseases. Therefore, this thesis has focused on understanding one cell type within the bladd ...

EPFL2012

Engineered insulin-like growth factor-1 for improved smooth muscle regeneration

Jeffrey Alan Hubbell, Peter Frey, Kristen Lorentz, Lirong Yang

Insulin-like growth factor-1 (IGF-1) has been shown to induce potent mitogenic responses in various cell types, yet its sustained local delivery is still an underdeveloped domain in the clinic. We report here an engineered IGF-1 that facilitates extended l ...

2012

Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut

Bruno Lemaitre, Sveta Chakrabarti

Typically, immune responses control the pathogen, while repair and stress pathways limit damage caused by pathogenesis. The relative contribution of damage to the outcome of pathogenesis and the mechanistic links between the immune and repair pathways are ...

Cell Press2012

Loss of Cutaneous TSLP-Dependent Immune Responses Skews the Balance of Inflammation from Tumor Protective to Tumor Promoting

Freddy Radtke, Ute Koch, Matteo Di Piazza, Craig Nowell

Inflammation can promote or inhibit cancer progression. In this study we have addressed the role of the proinflammatory cytokine thymic stromal lymphopoietin (TSLP) during skin carcinogenesis. Using conditional loss- and gain-of-function mouse models for N ...

Elsevier2012

High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells

Jeffrey Alan Hubbell, Peter Frey, Lionel Ambroise Micol

Tissue-engineered grafts for the urinary tract are being investigated for the potential treatment of several urologic diseases. These grafts, predominantly tubular-shaped, usually require in vitro culture prior to implantation to allow cell engraftment on ...

Elsevier2011

Hydrogels for Urinary Tract Tissue Engineering

Lionel Ambroise Micol

Several urinary tract disorders, such as hypospadias or bladder cancer, require reconstructive surgery to preserve the patient's quality of life and at times even save their life. The current available surgical treatments, involving grafts from other tissu ...

EPFL2011

A collagen-poly(lactic acid-co-epsilon-caprolactone) hybrid scaffold for bladder tissue regeneration

Florian Maria Wurm, Peter Frey, Jeffrey Alan Hubbell, Lionel Ambroise Micol

Scaffold materials should favor cell attachment and proliferation, and provide designable 3D structures with appropriate mechanical strength. Collagen matrices have proven to be beneficial scaffolds for tissue regeneration. However, apart from small intest ...

2011

Characterization of N-cadherin unbinding properties in non-malignant (HCV29) and malignant (T24) bladder cells

Andrzej Kulik

The expression of N-cadherin, characteristic of various cancers, very often leads to changes in the cells' adhesive properties. Thus, we sought to find out if N-cadherin expressed in various, but cancer-related cells, differs in its functional properties t ...

Wiley-Blackwell2011

Compressed collagen gel: a novel scaffold for human bladder cells

Florian Maria Wurm, Jeffrey Alan Hubbell, Peter Frey

Collagen is highly conserved across species and has been used extensively for tissue regeneration; however, its mechanical properties are limited. A recent advance using plastic compression of collagen gels to achieve much higher concentrations significant ...

Wiley-Blackwell2010

High-magnification vascular imaging to reject false-positive sites in situ during Hexvix® fluorescence cystoscopy

Hubert van den Bergh, Georges Wagnières, Blaise Lovisa

Fluorescence imaging for detection of non-muscle-invasive bladder cancer is based on the selective production and accumulation of fluorescing porphyrins-mainly, protoporphyrin IX-in cancerous tissues after the instillation of Hexvix (R). Although the sensi ...

SPIE Society of Photo-optical Instrumentation Engineers2010

Tissue engineering of the bladder : exploiting a 3D hydrogel for modulation of the smooth muscle cell phenotype

Catharina Adelöw

Stem cell use in bladder tissue engineering is a recently addressed area of investigation that has generated excitement as a novel way to restore and regenerate lost or damaged urinary bladder tissue. The remodelling of smooth muscle plays a significant ro ...

EPFL2009

Studies on scaffold design for tissue engineering applications : contribution of synthetic, natural and recombinant materials

The aim of tissue engineering is to regenerate tissue for the purpose of repairing or replacing diseased or injured tissue. Therefore, cells that are able to proliferate are seeded on scaffolds that provide mechanical stability and direct the three-dimensi ...

EPFL2009

Photodynamic diagnostics in the urinary tract. Consensus paper of the Working Group for Oncology of the German Society for Urology

Hubert van den Bergh

Because of the frequency of occurrence and the long protracted course, bladder carcinoma is the most expensive solid tumor in terms of costs, from diagnosis to death of the patient. The most important cost factor within the total cost is the treatment of r ...

Springer Verlag2008

Hexyl-aminolevulinate-mediated photodynamic therapy: how to spare normal urothelium. An in vitro approach

Hubert van den Bergh, Pavel Kucera, Céline Ritter Schenk

Photodynamic therapy (PDT) of superficial bladder cancer may cause damages to the normal surrounding bladder wall. Prevention of these is important for bladder healing. We studied the influence of photosensitizer concentration, irradiation parameters, and ...

Wiley-Blackwell2007

Clinical assessment of fluorescence cystoscopy during transurethral bladder resection in superficial bladder cancer

Hubert van den Bergh, Georges Wagnières

The prognosis of superficial bladder cancer in terms of recurrence and disease progression is related to bladder tumor multiplicity and the presence of concomitant "plane" tumors such as high-grade dysplasia and carcinoma in situ. This study in 33 patients ...

1997