Publications associées à Affinity maturation

Preclinical development of a humanized chimeric antigen receptor against B-cell maturation antigen for multiple myeloma

Multiple myeloma is a prevalent and incurable disease, despite the development of new and effective drugs. The recent development of chimeric antigen receptor (CAR)T cells has shown impressive results in the treatment of patients with relapsed or refractor ...

FERRATA STORTI FOUNDATION2021

Epitope Mapping and Fine Specificity of Human T and B Cell Responses for Novel Candidate Blood-Stage Malaria Vaccine P27A

Bruno Emanuel Ferreira De Sousa Correia, Che Yang, Daniel Guignard

P27A is a novel synthetic malaria vaccine candidate derived from the blood stage Plasmodium falciparum protein Trophozoite Exported Protein 1 (TEX1/PFF0165c). In phase 1a/1b clinical trials in malaria unexposed adults in Switzerland and in malaria pre-expo ...

2020

The adipokine NimrodB5 regulates peripheral hematopoiesis in Drosophila

Bruno Lemaitre, Jean Philippe Boquete, Jan Paul Dudzic, Elodie Julie Virginie Ramond, Bianca Marie Petrignani

In animals, growth is regulated by the complex interplay between paracrine and endocrine signals. When food is scarce, tissues compete for nutrients, leading to critical resource allocation and prioritization. Little is known about how the immune system ma ...

WILEY2020

Engineering cancer vaccines using stimuli-responsive biomaterials

Li Tang, Yugang Guo, Yu Zhao

Cancer vaccines aimed at expanding the pool or increasing the activity of tumor-specific T cells against malignancies is an important immunotherapy modality that has been extensively pursued in the past decades. However, the clinical efficacy of cancer vac ...

2018

Phage selection of chemically stabilized alpha-helical peptide ligands

Christian Heinis, Jörg Hülsken, Pierre Vincent Dessen, Philippe Diderich, Davide Bertoldo, Maola Khan Mohammad Golam

Short alpha-helical peptides stabilized by linkages between constituent amino acids offer an attractive format for ligand development. In recent years, a range of excellent ligands based on stabilized alpha-helices were generated by rational design using a ...

2016

Screening of structurally diverse bicyclic peptide libraries by phage display

Davide Bertoldo

Bicyclic peptides are an attractive molecule format for the development of therapeutics and research tools. Our laboratory is specialized on the development of bicyclic peptide ligands by phage display. In brief, libraries of randompeptides each containing ...

EPFL2016

Mechanisms of tolerance induction by erythrocyte-targeted antigens

Alizée Jéléna Grimm

Immune-mediated disorders such as autoimmune diseases, graft-versus-host disease or allergies, are a major contemporary burden. Despite significant advances in the field of immunological tolerance, immunosuppressive drugs are still the current hallmark for ...

EPFL2016

A droplet-based heterogeneous immunoassay for screening single cells secreting antigen-specific antibodies

Samin Akbari, Tohid Pirbodaghi

High throughput heterogeneous immunoassays that screen antigen-specific antibody secreting cells are essential to accelerate monoclonal antibody discovery for therapeutic applications. Here, we introduce a heterogeneous single cell immunoassay based on alg ...

Royal Soc Chemistry2014

Development of bicyclic peptide Her2 binders and phage selection of alpha-helical peptide ligands

Philippe Diderich

Conformationally constrained peptide ligands offer an attractive format for the development of therapeutics. They can bind with high affinity and selectivity to protein targets, they are small enough to diffuse into tissues, they can be synthesized chemica ...

EPFL2014

Phage display libraries of differently sized bicyclic peptides

Christian Heinis, Inmaculada Rentero Rebollo, Alessandro Angelini

Phage selections with combinatorial libraries of uniformly sized bicyclic peptides have recently yielded potent and selective binders of several protein targets. In this work we varied in a combinatorial fashion the ring sizes of bicyclic peptides in phage ...

Royal Soc Chemistry2013

TLR4- and TRIF-dependent stimulation of B lymphocytes by peptide liposomes enables T cell-independent isotype switch in mice

Pedro Miguel Nunes Pereira de Almeida Reis, Andrea Pfeifer, Marija Vukicevic, Andreas Muhs, Valérie Gafner, Anna Lucia Buccarello

Immunoglobulin class switching from IgM to IgG in response to peptides is generally T cell-dependent and vaccination in T cell-deficient individuals is inefficient. We show that a vaccine consisting of a dense array of peptides on liposomes induced peptide ...

Amer Soc Hematology2013

Notch Signaling Regulates Follicular Helper T Cell Differentiation

Freddy Radtke, Ute Koch, Nicolas Fasnacht, Anne Wilson, Floriane Auderset

Follicular helper T (TFH) cells are specialized in providing help for B cell differentiation and Ab secretion. Several positive and negative regulators of TFH cell differentiation have been described but their control is not fully understood. In this study ...

American Association of Immunologists ; Oxford University Press2013

Exploiting immunofunctional nanoparticles as vaccines and synthetic pathogens

Sai Reddy

The emergence of recombinant DNA technology has shifted research towards developing subunit vaccines, which use protein or peptide antigens instead of live/attenuated pathogens. Recent research has also shown that the best way to get sustained immunity is ...

EPFL2008

Fast directed evolution of non-immunoglobulin proteins by somatic hypermutation in immune cells

Kai Johnsson, Christian Heinis

A review. New, improved phenotypes. Somatic hypermutation in immune cells can be used for the fast directed evolution of proteins other than Igs (e.g., autofluorescent proteins). The target gene is inserted into the genome of activated B lymphocytes where ...

2005